INSTRUCTION

M O D E L MF-M

NARISHIGE] NARISHIGE SCIENTIFIC INSTRUMENT LAB. J A P A N , 9-28 KASUYA 4-CHOME SETAGAYA-KU. TOKYO 157. JAPAN

PH0NE(INT-L)81-3-3308-8233, FAX(INT-L)81 -3-3308-2005 CABLE:NARISHIGELABO, TELEX, NARISHIGE J27781

* ^ l l Ittlil c V E $ 0 ^ 4 T i 9 » 2 8 - ^ =rl57 I'Horjl l|-< ^308-8233 FAX 03-3308-2005

G e n e r a l

Thank you for purchasing the Narishige MF-79 Microforge (for miniature tools). This instrument is designed for processing the tips of glass micropipettes produced by a pipette puller. The MF-79 is perfect for producing micropipettes for injection, cutters, spoons and other miniature tools. Fire-polishing is also available to make pipette tips round and smooth.

Names of t h e p r i n c i p a l p a r t s ® Rotating Disc

® X-Y Stage

® Heater Holder

® TransQitting Iliuninator

® Heater Manipulator

(D IlIuDination Control

<3) Footsgitch Connector

® Footswitch

® Poser Switch

® Pipette Manipulator

® Air Nozzle

® Eyepieces

® Hicroscop

® Air Tube

S p e c i f i c a t i o n s MF-79 Specifications Microforge (for miniature tools)

Power Box : Power Input - lOOV AC

Output Terminal - 1/2/3/4/6V 3A

Hicroscope : Objectives - lOX, 4X

Eyepieces - WFIOX

Heater Manipulator

Heater

Heater Aoneter Scale

Ditensions

Weight

Accessories

X-Axis/30mm (Coarse), Y-Axis/23mm (Coarse), Z-Axis/22mm (Fine), Joystick for X-Y Plane (Fine)

Platinum Wire (100;im)

0 - 3A

350 (if) X 205 (D) X 255 (H) mm

9.8 Kg

Footswitch 1 EA

Heater 1 EA

Lamp 1 EA

- 2 -

C o n s t r u c t i o n The MF-79 consists of the following sections:

1. Main body base and Power Sources Housing

1) The base is made from light alloy plate and equipped with each support rod.

2) The housing has the power sources for heater and built-in illumination.

3) Glass pipette manipulator and transmitting illuminator with associated control are mounted on the surface of the housing.

2 . Microscope

Binocular microscope and scope body.

3. Manipulators

1) For glass pipette (rotating disc type)

2) For heater (joystick type)

3) For air nozzle (ball joint type)

A s s e m b l y 1. Connect the cord at the rear of the transmitting illuminator, with the back

light plug.

2 . Assembling of the microsope. Next, when setting the binocular microscope to the scope body, put on the power switch, and switch on the transmitting illuminator (back light) and set the binocular microscope so that the intersecting point of the cross line of the rotating disc of the pipette manipulator can be focussed on the center of visual field.

3. Set up the joystick heater manipulator in the support rod at the right of the microscope. This manipulator is arranged to move the heater element in two planes (forward up and down) with rack and pinion adjustments and a fine screw drive in the lateral direction. The platinum heater is fixed in the head of the heater holder. Connect the cord of the heater with the heater clamp.

4 . The air ball joint manipulator can be installed in the support rod at the left of the microscope. This manipulator can move backward and forward. Adequate travel ranges are provided In all directions.

5 . Positioning of the heater and air nozzle.

1) Make up the heater form convenient to working the minute pipettes (Refer to Page 5).

2) The injection needle generally used as an air nozzle should have its thick­ness conforming to the volume of flowing air. Set the air nozzle at the appropriate position for operation.

- 3 -

I

O p e r a t i o n 1. Before turning the power switch on, please carry out strictly the following

items, in order to prevent the heater from burning out by overcurrent.

1) Set the dial for heater control at ' 0 '.

2) Put the heater switch at " OFF '.

2 . Put the power switch on, pilot lamp on.

1) Appropriate illuminance can be provided from darkness to full brilliancy, by the dial for illumination control.

2) Put the micropipette (pulled with the microelectrode puller) in the pipette fixing groove of the pipette manipulator and set the forming portion to be worked of the pipette at the center of visual field.

3) Set the heater switch at IV and put up gradually the dial for heater control until the radiation of the platinum heater becomes yellow bright. If it does not become yellow bright even at IV. Turn the dial at 2V or 3V.

(fhen the platinum heater is burnt out, put a new heater into the head point of the heater holder as shown below.

''"" I I I

•hithi-'",:.,;- '-^-'.^^-!:".. • • •• ••: .. .-•••••..,• • ••. - ' , • : -„- . - i . . ' - •-

.>^(^d^>}^c -::>::-:[^'^-^f--v^y^/.--':;i:^:-:ir^^^^

4) Th^ pipette flri^il^e;*brl(dvP5e(i^ with yoiir JrigKt -iiand;-rotating the-diaF for with your l e f t - ­hand, and holding the air tube in your mouth.

G u i d e l i n e s f o r u s e N O T E : The MF-79 is a precision instrument. It is intended to produce glass

pipettes in the micron size range.

Please observe the following guidelines when operating the HF-79.

1. Handle carefully and avoid dropping or jarring.

2 . Do not disassemble the unit.

3 . Keep foreign materials i.e. blood, drugs etc. from contacting the equipment. Keep out of direct sunlight.

4 . Please call a representative if you have any questions regarding use of the MF-79.

- 4 -

P r o c e s s i n g T e c h n i q u e T e c h n i q u e # 1

Various techniques can be used to produce various microtools such as:

#Heat processing #Cooling by air #Micronanipulating the pipette •Rotating the pipette with the rotating disk •Controlling footswitch

1. H o w t o b e n d t h e p i p e t t e : If the heater filament is positioned close to the pipette shaft, the pipette will bend with the upward flow of hot air. Rotating the pipette with the rotating disk will maximize the bend.

2 . H o w t o m a k e a f i n e p o i n t : A . Vhen the heater filament is placed so that it touches the pipette tip.

the glass tip melts. While the melting take place, pull the heater filament away from the pipette quickly as shown in the illustration. This results in the tip of a very fine needle point.

B . When the heater filament is close to the pipette, the pipette will bend with the upward flow of hot air. Then use step A. to draw the tip to a very fine needle point. The MF-79 can produce a variety of microtools with various shapes. Selecting the proper heater filament configurations is very important. Also, using the microscope positioning knobs properly is essential.

- 5 -

P r o c e s s i n g T e c h n i q u e T e c h n i q u e # 2

3. H o w t o m a k e a r i n sr: A ring can be fabricated by using the upward flow of hot air and rotating the pipette with a rotating disk. A ring will be completed by repeating the procedure of bending.

4. H o w t o m a k e a d e n t : Heating and operating the pipette as shown in the illustration will result in a transformation of the tip. Turning off the heater filement and cooling will cure the glass.

5. H o w t o m a k e a c l o s e d n e e d l e p o i n t : Add weight,such as clay,to the end of the pipette. The tip pf the pipette will separate with the combination of heat and gravity.

-6

Biotechnology is reaching higher levels and becoming more diverse. Nowadays, biotechnology is expanding its range of applications into a wide variety of industries, from agriculture, food products, chemicals, phannaceutics. and medicine to energy production. Narishige is continuing its product development based on its fundamental philosophy of making it easy to obtain the highly precise micromanipulation required for research in these fields.

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Introduction

Thank you for purchasing the Narishige MN-3 manipulator The MN-3 Is a three dimensional direct drive coarse positioning manipulator for the Initial rapid positioning of the micropipette prior to fine micromanipulation. It Is used In combination with the drive unit of various hydraulic remote control micromanipulators such as the MO-202 (Joystick 3 dimensional hydraulic micromanipulator) or the MO-303 (Ultra fine, low drift 3 dimensional hydraulic micromanipulator). The MN-3 manipulator Is made of light alloy and Is of a compact design. It can be mounted on either the right hand or left hand side of a microscope and is easily attached to the microscope mounting adaptor with two fixing screws (see photograph t)eneath). The MN-3 manipulator Is for use with microscope mounting adaptors that attach at the stage level of the microscope. Vibration transmitted by the operator Is minimized and the movements of the mechanical stage of the microscope are not inhibited. The P-3 fixing plate is fitted to the main body of the MN-3 and then the drive unit of the hydraulic remote control micromanipulator is fitted to the P-3 fixing plate. The configuration of the complete assembly is designed to ensure optimum performance by keeping the distance between the fixing point of the hydraulic drive unit and the tip ot the micropipette to a minimum. After the MN-3 manipulator has been used for the initial coarse positioning of the micropipette each of Its 3 axes can be clamped by means of locking screws. This ensures any subsequent movement or vibration from the MN-3 manipulator is totally eliminated. If the P-3 fixing plate, supplied with the MN-3 manipulator, is replaced with a P-2 fixing plate, the MN-3 can be converted to an MN-2 for use with hydraulic micromanipulators such as the MO-202 and MO-303. The MN-2 Is the type of coarse positioning manipulator for use when the micromanipulator Is mounted to the lighting support arm of Inverted microscopes. If the P-3 fixing plate, supplied with the MN-3 manipulator, is replaced with a P-1 fixing plate, the MN-3 can be converted to the equivalent of an MN-1 type manipulator. The MN-1 manipulator Is the type required for use with the older types of hydraulic manipulators.

Left Hand Use

1 ^ ^ ^ ^ W ^ J ^

p-3 1 ^^^S ^^^s ^ ^ ^ ^

J j ^ ^ ^

Right Hand Use

1^' ' "

^ ^ ^

1 ^ ^ ^ [^^....T

l ~ w^

Items Supplied

The MN-3 manipulator Is made up of parts shown In the photograph below. Please check all the parts are Included.

1. Main Unit

2. P-3 fixing plate vAVn height adjustment (see right hand photograph) made up of the following items.

a. Height adjustment plate Qty 1

b. Spacer Qty 2

c. Fixing screw for main unit (6 X 20mm) Qty 2

d. Fixing screw for plates a. and b. (6 X 40mm) Qty 2

e. Allen key (for 6 mm fixing screw) Qty 1

P-3

a

•

HA^b'

(5)000 id

e

-2-

The Components and Their Function

(For right hand use)

1. X-Axis Control Knob Rotation of the control knob moves the micropipette in the X direction (right and left). Clockwise rotation moves the micnDpipette to the right and counterclockwise rotaion moves it to the lefL The range of movement is 30 mm.

2. Y-Axis Control Knob Rotation of the control knob moves the micropipette in the Y direction (forwards and backwards). Clockwise rotation moves the micropipette backwards and counterclockwise rotation moves It fon^/ards. The range of movement is 30 mm.

3. Z-Axis Control Knob Rotation of the control knob moves the micropipette in the Z direction (up and down). Clockwise rotation moves the micropipette downwards and counterclockwise rotation moves It upwards. The range of movement is 25 mm.

4. Z-Axis Clamp Tighten the locking screw after coarse positioning of the micropipette. Do not attempt to adjust the Z-axis when the movement is clamped.

5. Y-Axis Clamp Tighten the locking screw after coarse positioning of the micropipette. Do not attempt to adjust the Y-axis when the movement is clamped.

-3-

6. Holes tor Fixing Screws to Mounting Adaptor Attach the MN-3 manipulator to the mounting adaptor and tighten the two screws securely to prevent vibration.

7. P-3 Fixing Plate This Is used to attach the drive unit of some types of hydraulic micro­manipulators (such as the MO-202/203 or the MO-302/302) to the MN-3 manipulator. Coarse positioning of the fine hydraulic micromanipulator is carried out with the MN-3 manipulator. The positioning of the P-3 fixing plate enables the height of hydraulic unit above the MN-3 manipulator to be adjusted. Spacers are also provided. The spacers are used if necessary.

8. Holes for Fixing Bar The fixing bar of the hydraulic micromanipulator drive unit Is Inserted Into either of these holes. Select the hole that achieves the minimum distance from the fixing bar to the micropipette tip.

9. Locking Screw for Fixing Bar Lock the hydraulic micromanipulator drive unit fixing bar into the appropriate hole (8).

10. X-Axis Clamp. Tighten the locking screw after coarse positioning of the micropipette. Do not attempt to adjust the X-axis when the movement is clamped.

Assembly Instructions

Assembly and Attachment of P-3 Fixing Plate

Right Hand Use

Hole for Fixing Bar

Left Hand Use

Refer to left hand photograph Attach the P-3 fixing plate to the main body of the MN-3 manipulator by means of the two 6 X 20 mm fixing screws. If it is necessary to use the spacers put them tjetween the main body and the P-3 fixing plate and use the 6 x 40 mm fixing screws. Clamp the P-3 fixing plate at the appropriate height.

Attach the microscope mounting adaptor to the microscope. Attach the MN-3 in the appropriate position, to the right hand side of the microscope mounting adaptor with the two 6 mm fixing screws. Fit the hydraulic micromanipulator drive unit to the MN-3 by inserting and clamping the fixing bar into the hole In the P-3 fixing plate.

Attach the microscope mounting adaptor to the microscope. Attach the MN-3 in the appropriate position to the left hand side of the microscope mounting adaptor with two 6 mm fixing scr6ws Fit the hydraulic micromanipulator drive unit to the MN-3 by inserting and clamping the fixing bar into the hole in the P-3 fixing plate.

Precautions

The MN-3 is a high precision piece of equipment which, before despatch has been carefully adjusted in our factory. Please observe the following:

1. Handle with care and avoid any Jolts or knocks.

2. Do not attempt to adjust or dismantle the equipment. In the event of the equipment requiring any adjustment please retum it to your nearest authorized repair center.

3. Avoid spillage on the equipment In the event of such an accident occurring clean immediately with an organic solvent such as alcohol. Do not sterilize by boiling.

4.. When not in use, keep the equipment away from direct sunlight and protect from dust If it is necessary to clean the equipment use a dry soft cloth.

If you do have any problems, questions or comments please contact us.

Specification

The MN-3 specification

Three dimensional direct drive coarse positionir)g manipulator unit

Range of movement:

Material:

Weight-

Accessory

X-axis coarse movement 30 mm Y-axis coarse movement 30 mm Z-axis coarse trravement 25 mm

Light alloy

Approx. 310g

P-3 Fixing plate with height adjustment (Weight Approx. 110g)

25mm

I

Biotechnology is reaching higher levels and becoming more diverse. Nowadays, biotechnology is expanding its range of applications into a wide variety of industries, from agriculture, food products, chemicals, pharmaceutics, and medicine to energy production. Narishige is continuing its product development based on its fundamental philosophy of making it easy to obtain the highly precise micromanipulation required for research in these fields.

mmm ©©..LTD.

i I n s t r u c t i o n M a n u a l

f o r

M O - 2 0 3 T h r e e D i m e n s i o n a l H y d r a u l i c

M i c r e m a n i p u 1 a l o r

Thank you for purchasing the Narishige MlJ-203 Hydraul ic Micromanipulator. The

MO-203 has been built with pride and should give you years of trouble-free op­

eration. This manual is designed to help yoii get. maximum performance from your

MO-203. If after reading it you require further assistance, please never hesi­

tate to call us directly to speak wilh one of our applications specialists.

Unpacking

The MO-203 has been carefully packed and inspected, a picture of the package

contents is shown below. You should have one (1) of each of the items shown.

Should any of the parts arrive in a damaged condition, contact your common car­

rier immediately. Oo not call us since corrective action must start wilh the

carrier. Be assured that we will cooperate completely lo help conect the

problem and to have you oiierational quickly.

Connecting Rod (lOmm)...Connected with Headstage I Control ler r-. .-.-.

Ball Joint (B-8C)

The MO-203 is a compact, vibration-free, three dimensional hydraulic micromanip­

ulator. Smooth microdrive movement through a lOinm range is remotely and inde­

pendently controlled for each of the X, V and Z a.ves. The smooth vibration-free

movement of the microdrive makes the MO-203 ideal for inserting microelectrodes

into small cells or culture.

This manipulator is light-weight and is designed to be mounted on a microscope

using specific adaptors which enfiance the vibration-free movement.

This MO-203 manipulator is designed for both Right hand and Left hand use.

Ad,iiist both the headstage and controller so that the printed "R" is facing the

user when Right hand use applies. Do the same for Left hand use while making

sure that the printed "L" is facing the user.

In order lo connect the headstage wilh ihe coarse movement manipulator (MN-2),

you should screw the connecting rod into the back of the headstage. In case of

Right liand use, the connecting rod should he screwed into lite hole indiea led

"R". In case of t.efl hand use, screw the connecting rod into the hole indicated

"t.".

Piincipal Parts of the Headstage

When the headstage is positioned to face "R" in front, you can u.se it for Right

hand use. When positioning to face "L" in front, you can use it for Left hand

use.

Right Hand Use

(1) Ball joint B-8C

(2) X-unit for Right hand use, V-unit for Left hand use

(3) V-unil for Right hand use, X-unit for Left hand use

(4) Z-unit

i S ) Pipette Holder H-7

(0) Pipette Holder Fi.ving Screw

(7) Pipette Fixing Screw

:3-

Principal Parts of the Controller

(1) Z-axis Control Knob

(2) X-axis Control Knob for Right hand use V-axis Control Knob for Left hand use

(3) Operation Scale

(4) Controller Housing

(5) V-axis Control Knob for Right tiand use X-axis Control Knob for Left hand use

Right Hand Use

T'

(4),

(5)

- 4 -

The Headstage

The headstage of the MO-203 is composed of three microdrive units which are

assembled at right angles from each other, so as to represent X, Y and Z move­

ments. A micropipette can be moved to any point within a lOmm sided cube by

making use of hydraulic pressure which is directed from a remotly located

controller.

The following parts are necessary when mounting the headstage to various micro­

scopes .

(1) Adaptor for type of Microscope (See catalog for applicable part nufflber(s))

(2) MN-2 (or MN-3) Coarse movement Manipulator I

The headstage must be mounted so that the printed "R"|is facing the user when

Right hand use applies. In case of Left hand use, turn the headstage to the

left 90 degrees so that the printed "L" is facing the |user.

i In order to connect the headstage with the coarse movement manipulator MN-2, you

must screw the supplied connecting rod into the back of the headstage. When

Right hand use applies, screw the connecting rod int0|the hole marked "R".

For Left hand use, screw the connecting rod into the hlole marked "L".

I

After mounting the headstage completely to the microscope, rotate the knobs

of the Controller so as to make sure that each knob is controlling the correct

axis. I I

The cpntrollgr I

The Controller is made up of three operation knobs (1) (2) (5) which control

independent movement along all three axes. \

I

- 5 -

Working Distance

Headstage.* X-axis

Y-axis I

Z-axis j

Fine Movement

Movable Distance 10mm each

Controller: X, Y and Z Direction

Fine Movement

Controllable Distance lOmm each

One rotation of the knob results in the movement of the microdrive

by 500 microns, while the minimum scale of the control knob for X,

Y and Z stands for 2 microns.

(1) Pipette Holder

(2) Ball Joint

Accesspries

H-7

B-8C

1 ea

1 ea

Handling and Maintenance of the Instrument

This precision instrument is delicate and should be handled with care. Avoid

any shock to the instrument. Be aware that pulling on the hydraulic lines can

result in damaging the sealed hydraulic system. Keep in mind that this system

is sensitive to temperature change. To minimize unwanted movement of the head-

stage, keep MO-203 in a stable temperature environment away from drafts and sun.

After long terra use, the oil may change color. This is due to oxidation of the

oil. This change should not affect the instrument's performance.

After each use turn all three vernier controls to their zero positions in order

to prevent excessive oil leakage through diaphragm. (A small amount of oil leak­

age is inevitable and should not be regarded as a defect in the device.)

- 6 -

For best results when operating the MO-203 each of the vernier controls should

be at the mid-range of their 10mm travel.

Never attempt to disassemble a control cylinder. If so, this will make re­

adjustment very difficult.

The oil containers of the microdrives and controller are of a cartridge design

so that repair is a matter of replacing the cartridge.

If problems occur with the system, please do not attempt to repair, but rather

contact us and we will repair it for you.

- 7 -

Dav:i.d K Room 6H ATT Be.T 600 Hour

p i n f p . l d •424

L o b o r a t o r i e vbaxn Avenue

Murr.:iy Hi .1,1., N , j , 07974

QMSIiyCIBOEf^

M O - 2 0 3

i@.o [L'TP. 27-9, MINAMIKARASUYAMA 4-CHC)ME, SETAGAYA-KU, TOKYO, JAPAN.

TEL: (03)308-8383 CABLE :NARISHIGELABO

FACSI Ml LE: (03) 308-8700

INARISHICE J A P A N

r fe". t i l " ^ > ' V3

'157 M^SBff i f f lS|x i^ELiJ4T@27S9^ TEL.(03)308-8383 FAX.(03)308-B700

I n s t r u c t i o n M a n u a l

f o r

M O - 2 0 3 T h r e e D i m e n s i o n a l I l y d i a u l i c

M i c r o m a n i p u l a t o r

Tiiank you for purcliasiiig Ihe Narishige MO-203 Hydraulic Micromanipulator. Tlie

MO-203 has been bui l t with pride and should give you years of trouble-free op­

eration. This manual is designed to tielp you get maximum performance from your

MO-203. If after reading i t you require further assistance, please never liesi-

tate to call us directly to speak wilh one of our applications specialists.

Unpack i ng

Tlie MO-203 has been carefully paclved and inspected, a picture of liie package

conlents Is shown below. Vou should tiave one (1) of each of the items shown.

Sliould any of the parts arrive In a damaged condition, contact your common car­

r ier Immediately. Do not call us since corrective action must start with tiie

carrier. Be assured tliat we wi l l cooperate completely to lieip correct the

problem and to liave you oiierational quickly.

Connecting Rod (10mm)...Connected with Headstage Controller

a Headstage

'-' "^""^ Ball Joint (B-8C) ^ ^ ^ ^ t ^ ^ ^ ^ ^ ^ ^ '^'P^''*^ " '•^e'" <"-7)

- 1

'&^ v-fy'. ' .-^-.V' ::•••

n i i - •-••• t . / J . • . ' . ; « ' . • • • . . . • , • =

Br &

',w-.- , i / ^ •• • • ' , . !

I V , f • ',

Tiie MO-203 is a compaci, vibratlon-free, three dimensional hydraulic microinanip-

i i lalor. Smooth microdrive movement tiirough a lOinm range Is remotely and inde­

pendently controlled for eacli of tiie X, Y and Z a.xes. Tlie smooth vibration-free

movement of the microdrive makes tlie MO-203 ideal for inserting microelectrodes

into Kmall cel ls or culture.

Tills manipulator is 1 Iglil-welglit and is designed to be mounted on a microscope

using specific adaptors wiiicli enliance ttie vibration-free movement.

Tills MU-203 manipulator Is designed for botli KIglit hand and Left liaiul use.

Adjust bolli thf:- hcad.stage and controller so tliat tlie inlnled "K" is facin,". the

user when Ri,",lil iiaml wv' aii|»lies. Do tlie same for Left liaiul u.se KI INP iiiakin/i

sure tliat the I'linted "L" is far:Ing tlie user.

In order lo connect the headstage with ttie coarse movement numipuiator (MN-2),

you sJKHild screw tlie connecting rod into tiie liack of tiie tieadstage. in case of

Right lianii use, tlie connecting rod siiouid ite screwed into the hole Indicated

"R". in case oF Left tiaiiri iisr-, screw Llie connecting rod into the hole Indicaled

Principal Parts of tiie Headstage

Wiien tiie iieadstage is positioned to face "R" in front, you can use i t for Right

tiand use. Wlien positioning to face "L" in front, you can use i t for Left liand

use.

Rigiit Hand Use

(1) Bail Joint B-8C

(2) X-unit for Riglit iiaiid use, Y-unit for Left liand use

(3) Y-unit for Right liand use, X-unit for Left liand use

(a) Z-unit

(5) Pipette Holder H-T

(()) Pipette Holder Fixing Screw

(7) Pipette Fixing Screw

3-

Principal I'arts of tlie Controller

(1) Z-a.\is Control Knob

(2) X-axis Control Knob for Right liand use Y-axis Control Knob for Left liand use

(3) Operation Scale

(4) Controller Housing

(5) V-axis Control Knob for Right liand use X-axis Control Knob for Left hand use

Riglit Hand U.se

4

Tlie Headstage

The Iieadstage of tiie MO-203 is composed of three microdrive units which are

assembled at right angles from each other, so as to represent X, Y and Z move­

ments. A micropipette can be moved to any point within a 10mm sided cube by

making use of hydraulic pressure which is directed from a remotly located i

controller.

The following parts are necessary when mounting the headstage to various micro­

scopes.

(1) Adaptor for type of Microscope (See catalog for applicable part number(s))

(2) MN-2 (or MN-3) Coarse movement Manipulator

The headstage must be mounted so that tiie printed "R" is facing tlie user when

Right liand use applies, in case of Left hand use, turn the iieadstage to the

left 90 degrees so tiiat the printed "L" is facing the user.

in order to connect ttie headstage with the coarse movement manipulator MN-2, you

must screw the supplied connecting rod into the back of tiie headstage. When

Right liand use applies, screw the connecting rod into the lioie marked "R".

For Left liand use, screw the connecting rod into the hole marked "L".

After mounting the Iieadstage completely to tlie microscope, rotate the knobs

of tiie Controller so as to make sure that each knob is controlling the correct

axis.

The Controller

The Controller is made up of three operation knobs (1) (2) (5) which control

independent movement along all three axes.

- 5 -

Working Distance

Headstage: X-axis

Y-axis

Z-axis

Fine Movement

Movable Distance 10mm each

Controller: X, Y and Z Direction

Fine Movement

Controllable Distance 10mm each

One rotation of tlie knob results in tlie movement of the microdrive

by 500 microns, while the minimum scale of the control knob for X,

V and Z stands for 2 microns.

Accessories

(1) Pipette Holder H-7 1 ea

(2) Ball Joint B-8C 1 ea

Handling and Maintenance of the Instrument

This precision instrument is delicate and should be handled with care. Avoid

any siiock to tlie instrument. Be aware that pulling on the hydraulic lines can

result in damaging the sealed hydraulic system. Keep in mind tliat this system

is sensitive to temperature change. To minimize unwanted movement of the head-

stage, keep MO-203 in a stable temperature environment away from drafts and sun.

After long term use, the oil may change color. This is due to oxidation of ttie

oil. This cliange should not affect the instrument's performance.

After each use turn all three vernier controls to their zero positions in order

to prevent excessive oil leakage through diaphragm. (A small amount of oil leak­

age is inevitable and siiouid not be regarded as a defect in tlie device.)

- 6 -

For best results wlien operating the MO-203 each of the vernier controls should

be at the mid-range of their 10mm travel.

Never attempt to disassemble a control cylinder. If so, this will make re­

adjustment very difficult.

The oil containers of the microdrives and controller are of a cartridge design

so that repair is a matter of replacing tlie cartridge.

If problems occur with tlie system, please do not attempt to repair, but rather

contact us and we will repair it for you.

- 7 -

D a v i d K l e i n f e l d Room 6 H - 4 2 4 A T' T B e !l. 1 L. a b o r a t o r i e s 6 0 0 i l o 1..I. n t a i n A v o-/ n u e i i u r r a y H i l l y N , J , 0 7 9 7 4

INSTRUCTIONS

M N - 2

27-9, MINAMIKARASUYAMA 4-CHOME, SETAGAYA-KU, TOKYO, JAPAN.

TEL: (03)308-8383 CABLE: NARISHIGELABO

FACSIMILE; (03)308-8700

I NARISHIGE]

•n 9 n57 m^.^mm^^^mm[hATm^lm^^

TEL.(03)308-8383 FAX.(03)308-8700

I n s t r u c t i o n M a n u a l

f o r

M N - 2 ( M N - 3 ) T h r e e D i m e n s i o n a l

M a n i p u l a t o r

Thank you for purchasing the Narishige MN-2/MN-3 Micromanipulator. The MN-2/

MN-3 has been built with pride and should give you years of trouble-free oper­

ation. This manual is designed to help you get maximum performance from your

MN-2/MN-3. If after reading it you require further assistance, please never

hesitate to call us directly to speak with one of our applications specialists.

Unpacking

The MN-2/MN-3 has been careful lypackd and inspected. Should any of the parts

arrive in a damaged condition, contact your common carrier immediately. Do not

call us since corrective action must start with tlie carrier. Be assured that we

will cooperate completely to help correct the problem and to have you opera­

tional quickly.

MN-2(MN-3) Three Dimensional Manipulator

This manipulator is generally combined with the headstage of MO-202, MO-203 or

MO-204 Micromanipulators for coarse positioning a micropipette. This manipula­

tor can be mounted onto a microscope directly by using one of the Narishige Co.

manufactured mounting adaptors. MN-2 is designed to be mounted on the condensor

mounting attachments, while the MN-3 is designed to be mounted on microscope

stagis mounting adaptors.

The MN-2 uses a P-2 mounting attachment and the MN-3 uses a P-3 mounting attach­

ment, which is available for height adjustment.

-1

•J'

The Principal Parts

(6) Fixing liole for Mounting Adaptor

(7) Locking Screw for Z /gv

(8) P-2 (P-3)

(9) Fixing Screw for Headstage

(1) Operation Knob for Z-Axis

(2) Operation Knob for X-Axis

(3) Operation Knob for Y-Axis

(4) Locking Lever for X

(5) Locking Screw for Y

- 2 -

I '

Working Distance

30mm

30mm

25mra

Coarse Movement

Coarse Movement

Coarse Movement

1. X-direction

2. Y-direction

3. Z-direction

Features of the MN-2/MN-3

A. The main body is made of duralulminum and designed to be compact, lightweight

and sturdy.

B. Can be mounted on airabsit any microscope by making use of microscope mounting

adaptors available from Narishige Co. Ltd.

C. Locking levers for X, Y and Z directions are included for fixing the manip­

ulator in any position.

cesso

P-2

P-3

ines

1 ea included

1 ea included

MN-2

MN-3

Maintenance Notice

This manipulator has been adjusted at our factory for precise operation and no

field adjustments should;be required.

When handling, please observe the following:

1. Avoid any shock to the instrument.

2. Do not take the instrument apart.

3. Do not expose the instrument to dust, blood, chemicals, or direct

rays of sun.

Should any problem arise, please contact Narishige USA for a fast, courteous

assessment of your need.

- 3 -

r- g ^ NARISHIGE USA, INC.'S ONE YEAR LIMITED WARRANTY

Narishige USA, Inc. (NUSA) warrants purchased equipment (except as noted below) to be free of defects in material and workmanship under normal use and mainte­nance from the date of shipment for a period of one year, in the case of (I) fuses, lamps, light emitting diodes, and LCD's, and (ii) separately purchased replacement parts, the warranty period is 90 days. Consumable supplies, heating elements, and cables are warranted to be free of defects in material and workmanship at the time of shipment. Labor invoiced in connection with repairs performed at NUSA's facility is warranted for a period of 90 days from the date of shipment of the repaired equipment. All warranties herein extend to the original erid user only and are not assignable or transferable. All warranties herein shall not apply to any damage or defect which is caused by or due, directiy or Indirectly, to misuse, abuse, negligence, accident, unauthorized repair or repair parts. ALL WARRANTIES IMPLIED BY LAW INCLUDING ANY WARRANTY OF MER­CHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE ARE LIMITED TO THE DURATION OF THE EXPRESS WARRANTIES CONTAINED HEREIN. THE WARRANTIES HEREIN ARE EXPRESSLY IN LIEU OF ALL OTHER WAR­RANTIES. IN NO EVENT SHALL NUSA BE LIABLE FOR ANY INCIDENTAL OR CONSEQUENTrAlrDAMAGES FOR THE BREACH OF ANY WARRANTYfNUSA=s=-LIABILITY SHALL BE LIMITED TO REPAIRING OR REPLACING, AT NUSA's OPTION, ANY COMPONENT WHICH NUSA FINDS AT NUSA's SITE TO BE DEFECTIVE IN MATERIAL OR WORKMANSHIP NO WARRANTIES, WHETHER EXPRESS OR IMPLIED, INCLUDING ANY WAR­RANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE ARE MADE BY ANY DISTRIBUTOR OR DEALER OF THIS PRODUCT AND IN NO EVENT SHALL ANY DISTRIBUTOR OR DEALER OF THIS PRODUCT BE LIABLE FOR THE PAYMENT OF ANY INCIDENTAL OR CONSEQUENTIAL DAM­AGES OF ANY KIND WHATSOEVER. In order to obtain warranty service, the original end user should return this product to Narishige USA, Warranty Dept., One Plaza Road, Greenvale, New York 11548, together with a brief description of the problem that is sought to be remedied. In returning this product, the original end user niust repay ail postage, shipping, trans­portation, insurance and delivery costs. The return of the warranty registration form to NUSA will serve as a basis for estab­lishing proof of purchase and proof of purchase date by the original end user The failure of the original end user to return the warranty registration form will not affect ^ the rights hereunder, so long as the original erid user can establish proof of purchase III and proof of purchase date.

DEAR CUSTOMER:

We are pleased to have you join the growing family of customers who have purchased from Narishige USA, Inc. (NUSA). We appreciate the confidence which you have placed in us. It is this confidence which keeps us striving to provide the highest quality and value in all of our products.

Narishige USA, Inc. offers a broad range of complimentary products, some of which are described in the accompanying literature. Please feel free to contact us if we can help you with any of these products, or if you require appli­cation assistance with your current purchase.

So that your warranty will be valid, it is important that you complete and return the "Warranty Registration" card.

NARISHIGE USA, INC. ONE PLAZA ROAD • GREENVALE, NEW YORK 11548 • 516/621-4588

David Kleinfeld Room 6H~424 A "\ J B e :i. 1 1... 0 b o •!'• a t o r i e s 600 Mountain Avem.ie Murray Hilly N, J <. 07974

INSTRUCTIONS

M N - 2 C M N - 3 )

INARISHICE] [K]/^[^0©[nl[l©[i ©©.. [Lra 27-9. MINAMIKARASUYAMA 4-CHOME, SETAGAYA-KU, TOKYO, JAPAN.

TEL: (03)308-8383 CABLE: NARISHIGELABO

FACSIMILE: (03)308-8700

2? n57 m^lBttBaSEi^MLLl4Tg27S9^

TEL.(03)308-8383 FAX.(03)308-8700

M 0 - 2 0 3 M 3 X 7 c M ] ) ± ^ - l ' ^ a ^ ^ f a U - 5 ' - »lXSft0 MO-203 3-dimensional Hydraulic Micromanipulator Package

iXOitCtA-- ( 1 0 m m ) •••5gSigB*tt:tu-bjv_t*nTl^i^o" ~" ^-^^-^. . . \ ' : .T • '" •' " ' . Controller

Fixing Bar attached to ' the Head Stage

mmm-

^ ^

I n s t r u c t i o n M a n u a l

f o r

M N - 2 ( M N - 3 ) T h r e e D i m e n s i o n a l

M a n i p u l a t o r

Thank you for purchasing the Narishige MN-2/MN-3 Micromanipulator. The MN-2/

MN-3 has been built with pride and should give you years of trouble-free oper­

ation. This manual is designed to help you get maximum performance from your

MN-2/MN-3. If after reading it you require further assistance, please never

hesitate to call us directly to speak with one of our applications specialists.

Unpack i ng

The MN-2/MN-3 has been carefully packd and inspected. Should any of the parts

arrive in a damaged condition, contact your common carrier immediately. Do not

call us since corrective action must start with the carrier. Be assured that we

will cooperate completely to help correct the problem and to have you opera­

tional quickly.

MN-2(MN-3) Three Dimensional Manipulator

This manipulator is generally combined with the headstage of MO-202, MO-203 or

MO-204 Micromanipulators for coarse positioning a micropipette. This manipula­

tor can be mounted onto a microscope directly by using one of the Narishige Co.

manufactured mounting adaptors. MN-2 is designed to be mounted on the condensor

mounting attachments, while the MN-3 is designed to be mounted on microscope

stage mounting adaptors.

The MN-2 uses a P-2 mounting attachment and the MN-3 uses a P-3 mounting attach­

ment, which is available for height adjustment.

1 -

The Principal Parts

(6) Fixing hole for Mounting Adaptor

(7) Locking Screw for Z

(8) P-2 (P-3)

(9) Fixing Screw for Headstage

(1) Operation Knob for Z-Axis

(2) Operation Knob for X-Axis

(3) Operation Knob for Y-Axis

(4) Locking Lever for X

(5) Locking Screw for V

2-

Work i ng

30mm

30mm

25mm

Distance

Coarse Movement

Coarse Movement

Coarse Movement

1. x-direction

2. Y-direction

3. Z-direction

Features of the MN-2/MN-3

A. The main body is made of duralulminum and designed to be compact, lightweight

and sturdy.

B. Can be mounted on almost any microscope by making use of microscope mounting

adaptors available from Narishige Co. Ltd.

C. Locking levers for X, Y and Z directions are included for fixing the manip­

ulator in any position.

Accessories

P-2

P-3

1 ea included

1 ea included

MN-2

MN-3

Maintenance Notice

This manipulator has been adjusted at our factory for precise operation and no

field adjustments should be required.

When handling, please observe the following:

1. Avoid any shock to the instrument.

2. Do not take the instrument apart.

3. Do not expose the instrument to dust, blood, chemicals, or direct

rays of sun.

Should any problem arise, please contact us for a fast, courteous assessment

of your need.

- 3 -

P P - 8 3 G l a s s M i c r o e l e c t r o d e P u l l e r

Untline:

This nicroeleclrode poller is designed lo manuractarf micropipettes lor ase in patch clanp experinenlatioD. The tips of the electrodes produced by this poller can easily be fire polished so as to render them non-injnrioas to delicate cellolar nenbranes even when tight (gigaobn) patch seals are made.

The pp-83 allows for the independent control of the two sequential polls which are nsed to produce a patch pipette. The tenperatore of the fllament doring the first poll is controlled by the "first pnll heat adjostnent knob", and the length of the first poll can be controlled by the positioning of a nicroswilcb. In addition to nechanically slopping the fall of the puller assembly, activation of the nicroswilcb aotomaIlea IIy Inrns off the heating filanenl. Prior to the second poll, the experimenter can reposition the beat ing element lo Ihe base of Ihe narrowed portion of the glass capillary which was formed by the slretrbing of the molten glass during the first pall. After setting the "second pall heater adjustment knob", the second pull may be started. A patch micropipette is produced when the weight of the puller causes the molten glass to separate into two separate pieces.

Once the optimal heater adjustment settings have beeb detej-mined. the con sistent performance of the two independent heating circoits makes readjustment of the knobs unnecessary. The movable microforge feature of the PP-83 pre eludes the trooblesome repositioning of the delicate capillary after the first pull.

Specifications:

Power Source: 117 - 120 VOLT AC, (220 - 240 VOLTS AC)

Heater Voltage: 1.5 VOLTS

Current: I7A

Weight: b . h Kgs.

Dimensions: WIDOmm x DI80 mm x HI90 mm

( /)

pp-83 GLASS MICROELECTRODE PULLER NAMES

14. Micro-switch for heater

7 . Forge adjusting knob for up & down \ _

4 . 2nd heater adjusting dial

13. Weight hook

12. 1st pulling stoper

A daiameter of 1st puU and length, can be adjust by the 1st pulling stoper (12)

10. Kobes for forge

6 . Forge

9 . Hood

5 . Pipette holders

3 . 1st heater adjusting dial

8 . SUder

2 . Starter

15. Micro-switch for 1st stoper

1 • Power switch

11. Stoper adjust for forge This stoper can be adjust length of 2nd puUing. The position is around 3 / 5 better.

16. Micro-switch for 2nd stoper

pp-83 GLASS MICROELECTRODE PULLER

Make sure following points befor you start

You will loosen the pipette holder knobs (5), take out a tool. Check the heater adjusting dials (3) and (4) eire indicating 0. Check the forge part contact to the micro-switch (14) if not. adjust by the adjusting knob (7). This puller's voltage operated for AC-100 volts, therefor use attached adaptor.

* * * INSTRUCTION * * *

1) Put a glass tube, hold adove side tube only with (5) then the slider (8) moves up till stop it. Fix the tube below side by pipette holder (5).

2 ) Ajust the 1st pulling of length ' L ' by -stoper (12). set the micro-switch (15).

3 ) Tum on the power switch (1).

4 ) Cover the hood for forge (6).

5 ) Push the starter (2).

6 ) Turning right the 1st heater adjusting dial (3) mark around 3 8 4 3 for uoc PLu\TINUM ELEMENT. If use CANTAL WIRE mark around-58r

1 Put a glass tube, heater on

(10) ©

( 7 )

( 6 ) o

(10)

H

0

( 8 )

( 7 )

(10) / - ^ 00)

( 6 )

©

D 15

After set

L ti ix^\ fHi+i<^>

AditEt 1st I puning length

'.B <g>

2 1st step pulled slider moves dov«i to stoper

(10)

3 Tuming down the heater midle of pipette tum left micro-switch (15) Stater on (2) 4 After 2nd pulled

©

( 6 )

( 7 )

(10)

(10)

©

( 8 )

D 15

After 1st pull the

slider stop by

stoper (15)

( 7 )

©

( 6 )

(10)

3

( 8 )

16

reset the micro-switch (15)

\

7) The slider (8) moves down to 1st stoper (12).

\ 8 ) Turning down the heater (6) by the

forge adjust knob (7). set the forge stoper by adjust knob (11).

9) Turn left the micro-switch (15) for reset.

10) Push the starter (2) again.

11) Tuming right the 2nd heater adjusting dial (4) mark around 20-30 fui uAt PLi\TINUM ELEMENT. If use CANTAL WIRE mark around 4 1 . — < ? / .

11)—Tuming right the Ond heater adjujtiuft dial (4) mark around 3 8 3 0 for use PI ATTI ' M FIFMF^^^ ' - " ^ * ^ T ^ I mUE uiaik around 4 1 .

12) The shder (8) moves down.

How to adjust the forge position

(10) (10)

o o

o o

(10)

Q P

Loosen the knobs ( 1 0 ) and adjust front back.

^ ® ^

Also, left and right

How to adjust a diameter

A daiameter of 1st pull and length, can be adjust by the 1st pulling stoper (12)

After 1st puided pipette

A' is the 1st step position for heater

L

' A is the 2nd step position for heater

^ Tip of the pipette 3—6 /um

0 . 5/fN-

L is length of I s l puUed pipette

1 is length of the heater moved down

Befor start 2nd pull, waite 30/sec and the micro-switch (15) turn left to reset.

After heat-pohsh by micro-forge

The Names of the Principal Parts:

(1) Power switch

(2) Start botton

( ) ) I s t . heater adjustment d i a K f o r the f i r s t pn l l )

(4) 2nd. heater adjustment d i a K f o r the second pu l l )

(5) Pipette holders

(6) Forge

(7) Forge adjnstmeat knobUor vertical adjnstment)

(») Slider

(9) Hood

(lU; Forge aligmenl set screws

(li) Stopper for the second pull

i i l ) Stopper for the first poll

(Ij) Weight attachment hook

(14) Microswitch to initiate the first poll

(15) Microswitch lo terminale the first pnll

(lb) Microswitch to terminale the second pull

Notice:

Prior to operation, make sore the following items.

(1) Confirm that the beater adjustment dials (3)(4 ) indicate "0*.

(2) Bring the forge moanling into contact with the forge microswitch(14) by turning the forge adjostment knob(7).

(3) Apply the specific adaptor in case that the electric source is AI'II7 VOLTS, 220 VOLTS or 240 VOLTS, as this instrument is designed lo operate under the voltage of AC lUO VOLTS.

( 6 )

Operation:

(1) Insert a glass capillary through the filament and lock il in place by tightening the upper pipette holder(5). Move the slider(8) all the way op and fasten it in place by securing the capillary in Ihe lower pipette holder(3).

(2) As the first poll is terminated when the microswitch(l5) is contact ed by the base of the descending slider(S), the length and the dia meter of the narrowed portion of the capillary which resolls from the first pull can be adjusted by varying the position of the microswitch (15). This is accomplished by sliding the raicrosilch assembly and fix ing it in place by lightening the first pull stopper(I2).

(3) After adjusting the microswitch lo the position which gives electrodes the desired shape, make sure, belore initialing the first pnll, thai the microswilch(15) is set by swinging into the path of the slider.

«

(4) Torn the power swilch(l) to the ON position.

(5) Close the forge hood(9).

(6) Push the starter bntton(2).

(7) Turn the first pull healer adjustment dial(3) clockwise lo the setting. We recommend settings of 54 -5b for canlal filaments.

(8) The healed portion of the capillary become molten and the descending slider is hailed at the position of the stopper, activating the micros-swilcb(15) simultaneously.

(9) Following the completion of Ihe first poll, move the forge(6) into Ihe position for the second pull by turning Ihe forge's adjustment knob. Mark this position for snbseqoent second pulls with the stopper for the second pull.

(10) Swing the microswitch(15) out of the path of the slider(K).

(11) 30 seconds after the first pull has been completed, yoo may begin the second pull Push the starter bollon(2) again.

(12) Turn the beater adjustment dial for the second pnll(4) clockwise onlil the proper settings is reached. We recommend settings of 39 - 41 for canlal filanenl.

(13) The molten glass is caused lo separate into two separate pieces by the downward movement of the slider.

(14) After the second pull is completed, remove the two pieces of capillary lubing from the pipette holder(5).

(15) The capillary tube in the lower pipette holder can now be heat polished lo yield a "patch clamp" microelectrode.

(Ib) Turn the forge adjostment knob(7) so that the forge mounting returns its oppermost position in contact wilh the forge microswitch(l4).

(17) Return the microswilch(l5) to the original position.

a)

Accessories:

<'> Caotal filanenl

<2> Hexagonal ,rencb

.<^> Setscrew for fixing fHameol

<4) Fnse JA

(5) Forge

^ ^ > Class capillary

2 ea

1 ea

4 ea

2 ea

i ea

low ea

C 8 ;

Schematic Representation of the First and Second Pulls

Glass capill

Glass capilla after the fi pull

Capillary after the second pull

Setting of the Microswitch!

First pull filament position j I I 'Filament repositioning between Pulls

Second pull filament position

J I H - ^ L ^

Microswitch used to adjust the dimensions of the capillary

y which results from the.first pull.

Microswitch to terminate the second pull.

( 9 )

June, 1985

PP-83 Glass Microelectrode Puller

Actual Data pf Manufacturing the Electrodes for "Patch Clamp" and "Holding Pipette"

1.First heater adjusting dial (voltage adjustment) set at 65.

Second heater adjusting dial (voltage adjustment)

Time Diameter of the Tip

Scale 35

34

33

32

31

30

14 s e c .

15 "

16 "

28 "

40 "

4 Min. 30 S e c .

1 .2

2 . 4

1 0 . 0

1 8 . 0

1 9 . 0

2 8 . 0

micron 11

II

It

II

II

2. The diameter of the electrode tip may vary from above figures according to the time interval of the first pull and the second pull. Attention must be paid not to remove the microswitch (stopper) before the heater gets cool, otherwise the melted capillary may be extended further by remaining heat.

3. Above data may also change due to the stroke of the first pull.

4. Above data has been derived from the setting of the first pull voltage at 65 scale. The consequences may vary according to the first pull voltage.

5. As the diameter of the electrode may change due to the difference of the instrument itself, so please make use of the above data as a standard in case of making the electrodes.

Uo)

H.TRANS

L- -Oh

Slf"

CONT.I

C0NT.2

BUTTON

. I

a IJ

JUS/

HEATER

JLIS2

r£l cT

IJS3

P P - 8 3

NARISHIGE USA, INC.'S ONE YEAR LIMITED WARRANTY

Narishige USA, Inc. (NUSA) warrants purchased equipment (except as noted beiow) to be free of defects in material and workmanship under normal use and mainte­nance from the date of shipment for a period of one year In the case of (i) fuses, lamps, light emitting diodes, and LCD's, and (ii) separately purchased replacement parts, the warranty period is 90 days. Consumable supplies, heating elements, and cables are warranted to be free of defects in material and workmanship at the time of shipment. Labor invoiced in connection with repairs performed at NUSA's facility is warranted for a period of 90 days from the date of shipment of the repaired equipment. All warranties herein extend to the original end user only and are not assignable or transferable. All warranties herein shall not apply to any damage or defect which is caused by or due, directiy or indirectiy, to misuse, abuse, negligence, accident, unauthorized repair or repair parts. ALL WARRANTIES IMPLIED BY LAW INCLUDING ANY WARRANTY OF MER­CHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE ARE LIMITED TO THE DURATION OF THE EXPRESS WARRANTIES CONTAINED HEREIN. THE WARRANTIES HEREIN ARE EXPRESSLY IN LIEU OF ALL OTHER WAR­RANTIES. IN NO EVENT SHALL NUSA BE LIABLE FOR ANY INCIDENTAL OR

"CONSEQUENTIADDAMAGES FOR THE BREACH OF ANY-WARRANT-Y. NUSA's LIABILITY SHALL BE LIMITED TO REPAIRING OR REPLACING, AT NUSA's OPTION, ANY COMPONENT WHICH NUSA FINDS AT NUSAs SITE TO BE DEFECTIVE IN MATERIAL OR WORKMANSHIP NO WARRANTIES, WHETHER EXPRESS OR IMPLIED, INCLUDING ANY WAR­RANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE ARE MADE BY ANY DISTRIBUTOR OR DEALER OF THIS PRODUCT AND IN NO EVENT SHALL ANY DISTRIBUTOR OR DEALER OF THIS PRODUCT BE LIABLE FOR THE PAYMENT OF ANY INCIDENTAL OR CONSEQUENTIAL DAM­AGES OF ANY KIND WHATSOEVER. In order to obtain warranty service, the original end user should return thiis product to Narishige USA, Warranty Dept., One Plaza Road, Greenvale, New York 11548, together with a brief description of the problem that is sought to be remedied. In returning this product, the original end user must repay all postage, shipping, trans­portation, insurance and delivery costs. The return of the warranty registration form to NUSA will serve as a basis for estab­lishing proof of purchase and proof of purchase date by the original end user The

^ failure of the original end user to return the warranty registration form will not affect ^ WJ the rights hereunder, SO long as the original end user can establish proof of purchase III

and proof of purchase date.

WARRANTY REGISTRATION

Dealer (i( applicable) Product Purchased _

SeriaINo

Invoice No. _

Date of Delivery

Organization Name

Address

City

State : Zip Country _

Individual Name '. -.

In order to insure proper warranty service, user must fill out and return warranty registration form within 15 days. Detach, fold, and

return self-addressed card. i

uouejisjBati lustudmbg

v s n 8 ^ 9 U AN'9|BAue8J9

peoa Bzeid euQ 'ONI ' v s n 30IHSIUVN

3UBH

dNVlS

30V1d

DEAR CUSTOMER:

We are pleased to have you join the growing family of customers who have purchasedfrom Narishige USA, Inc. (NUSA). We appreciate the confidence which you have placed, in us. It is this confidence which keeps us striving to provide the highest quality and value in all of our products.

Narishige USA, Inc. offers a broad range of complimentary products, some of which are described in the accompanying literature. Please feel free to contact us.if we can help you with any of these products, or if you require appli­cation assistance with your current purchase.

So that your warranty will be valid, it is important that you complete and return the "Warranty Registration" card.

NARISHIGE USA, INC. ONE PLAZA ROAD • GREENVALE, NEW YORK 11548 • 516/621-4588

I n s t r u c t i o n M a n u a l

f o r

M O - 2 0 3 T h r e e D i m e n s i o n a l M y d i- a u 1 i c

M i c r o rn a n i p u 1 a t o r

Tiiank you for purciiasing tlie Narishige IiO-203 Hydraulic Micromanipulator. The

MO-203 lias heen l iu i l t with pride and should give you years of trouble-free op­

eration. This manual is designed to lielp you get maximum performance from your

MO-203. i f after reading i t you retiuire further assistance, please never hesi­

tate to call us direct ly lo speak with one of our applications specialists.

U'i£a.ckijig

The MO-203 lias been carefully paclved and inspected, a picture of tiie package

contents is sliown below. Vou sliould tiave one (1) of eacli of the items shown.

Siiouid any of the parts arrive in a damaged condition, contact your common car­

r ier iiimiedlately. Do not call us since corrective action must start with tlie

carrier. I e assured tliat we wi l l cooperate completely to lielp correct the

problem and to have you operational quickly.

Connecting Rod (10mm)...Connected with Headstage Controller

Ball Joint (B-8C)

- 1 -

Tlie MO-203 is a compaci, vibration-free, lliiee dimensional liydraulic micromanip­

ulator. Smooth microdrive movement tlirou,' ,h a 10mm ran,",e is remotely and inde­

pendently controlled for each of the X, Y and Z a.ves. The smooth vibration-free

movement of tlie microdrivt- makes the MO-203 ideal for inserting microelectrodes

into small cel ls or culture.

This mani|tiilal,or Is I ight-weiglit and is designed to lie mounted on a microscope

using specific adaptors wliicli enliance tfie vibration-free movement.

Tliis MO-203 manipulator is designed for tiotli Rigiit liand and Left liaml w v.,

.Adjust botti tlie iieadstage and controller so that ilie printed "R" is facin;'. the

user wlien Rir.lit I' 'nit ii'-.v applies. f)o tlie same for l,eft tiand use while iiiHl<in,".

sure thai tlie printed '"1." is facing ttie user.

In order lo connect tlie Iieadstage witli Ifie coarse movemenl manipulator (MN-2),

you siiouid screw tlie conneeting rod into the tiack of tiie iieadstage. In case of

Riglit tiand use, ttie connecting rod siiouid lie screwed into ttie tiole indicat'.'d

"R". In case of I.ePt bami usf:'. screw ttie eoimectiiig rod into tlie hole indicaled

— 9 —

Principal Parts of the Iieadstage

When tlie iieadstage is positioned to face "R" in front, you can use it for Right

hand use. When positioning to face "L" in front, you can use it for Left liand

use.

Right Hand t.lse

(1) l^all Joint B-8C

(2) X-unit for Riglit band u.se, Y-uiii l for Left band use

(3) Y-unit for Riglit band use, X-unit for Left liand use

(4) Z-unit

(5) Pipette Holder H-7

(fi) Pipette tiolder Fixing Screw

(7) Pipette Fixing Screw

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Principal Parts of the Controller

(1) Z-axis (ji i itrol Knob

(2) X-axis Control Knob for Riglit hand use Y-axis Control Knob for Left hand use

(3) Operation Scale

(4) Controller Housing

(fl) Y-axis Control Knob for Right liand use X-axis Control Knob for Left hand use

Riglit Hand t.lse

( 4 )

( 5 )

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The Headstage

The Iieadstage of the MO-203 is composed of three microdrive units which are

assembled at right angles from each other, so as to represent X, Y and Z move­

ments. A micropipette can be moved to any point within a 10mm sided cube by

making use of hydraulic pressure which is directed from a remotly located

controller.

The following parts are necessary when mounting the headstage to various micro­

scopes .

(1) Adaptor for type of Microscope (See catalog for applicable part nufflber(s))

(2) MN-2 (or MN-3) Coarse movement Manipulator

The headstage must be mounted so that the printed "R" is facing the user when

Right hand use applies. In case of Left hand use, turn the Iieadstage to the

left 90 degrees so that the printed "L" is facing the user.

In order to connect the headstage with the coarse movement manipulator MN-2, you

must screw the supplied connecting rod into the back of the headstage. When

Right hand use applies, screw the connecting rod into the hole marked "R".

For Left hand use, screw the connecting rod into the hole marked "L".

After mounting the Iieadstage completely to the microscope, rotate the knobs

of tlie Controller so as to make sure that each knob is controlling the correct

axis.

The Controller

The Controller is made up of three operation knobs (1) (2) (5) which control

independent movement along all three axes.

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Working Distance

Headstage: X-axis

Y-axis

Z-axis

Fine Movement

Movable Distance 10mm each

Controller! X, Y and Z Direction

Fine Movement

Controllable Distance 10mm each

One rotation of the knob results in the movement of the microdrive

by 500 microns, while the minimum scale of the control knob for X,

Y and Z stands for 2 microns.

Accessor i es

(1) Pipette Holder H-7 1 ea

(2) Ball Joint B-8C 1 ea

Handling and Maintenance of the Instrument

This precision instrument is delicate and should be handled with care. Avoid

any shock to the instrument. Be aware that pulling on the hydraulic lines can

result in damaging the sealed hydraulic system. Keep in mind that this system

is sensitive to temperature change. To minimize unwanted movement of the head-

stage, keep MO-203 in a stable temperature environment away from drafts and sun.

After long term use, the oil may change color. This is due to oxidation of the

oil. This cliange should not affect the instrument's performance.

After each use turn all three vernier controls to their zero positions in order

to prevent excessive oil leakage through diaphragm. (A small amount of oil leak­

age is inevitable and should not be regarded as a defect in tlie device.)

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For best results when operating the MO-203 each of the vernier controls should

be at the mid-range of their lOmm travel.

Never attempt to disassemble a control cylinder. If so, this will make re­

adjustment very difficult.

The oil containers of the microdrives and controller are of a cartridge design

so that repair is a matter of replacing the cartridge.

If problems occur with the system, please do not attempt to repair, but rather

contact us and we will repair it for you.

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