inhibiting complement c3 with apl-2 controls haemolysisand
TRANSCRIPT
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Inhibiting Complement C3 with APL-2 Controls Haemolysis and Increases Haemoglobin Levels in
Patients With Autoimmune Haemolytic Anemia (AIHA)
Interim Results From the PLAUDIT Clinical Trial Dr Bruno Fattizzo, MD
Fondazione IRCCS Ca’ Granda Policlinico Hospital Milan, Italy
Updates to slide 13 were made on 06/17/19
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DisclosuresBruno Fattizzo, MD
Consultation on AIHA for Apellis
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AIHA Is A Rare and Heterogeneous Disease
1. Anemia, hemolytic, acquired autoimmune. National Organization for Rare Disorders Web site. https://rarediseases.org/rare-diseases/anemia-hemolytic-acquired-autoimmune/. Accessed March 1, 2018. 2. Berentsen S. Role of complement in autoimmune hemolytic anemia. Transfus Med Hemother. 2015;42(5):303-310. 3. Sonikpreet S, Oberoi G, Kumar S. Case report: autoimmune hemolytic anemia with venous thromboembolism, a common complication of a rare disease. Blood. 2014;123:4869. 4. Bass GF, Tuscano ET, Tuscano JM. Diagnosis and classification of autoimmune hemolytic anemia. Autoimmun Rev. 2014;13(4-5):560-564. 5. Barcellini W. Pitfalls in the diagnosis of autoimmune haemolytic anaemia. Blood Transfus. 2015;13(1):3-5.
• Incidence in adults of 0.8–3 per 100.000/year
• Middle-age female predominance• Pathogenesis autoantibodies
against erythrocytes• Diagnosis DAT test (I,II,III level),
steroid response
Characterized by the premature haemolysis of red blood cells (RBCs) by autoantibodies.1
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Complement C3 Is Central in the Disease Pathology of AIHA
Extravascular Haemolysis is Reported To Be The Unique Route of Haemolysis in wAIHA2
Haemolysis in CAD Appears To Be Complement-Mediated1-3
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Steroids
Rituximab
Splenectomy
Immune-suppression
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Current Treatment Options
Rituximab
Alone or in association
W. Barcellini, Current treatment strategies in autoimmune hemolytic disorders. Expert Rev Hematol. 2015S. Berentsen, Bendamustine plus rituximab for chronic cold agglutinin disease (...). Blood. 2017
EPO, PEX, ECULIZUMAB, ANTI-C3, BORTEZOMIB, BMT…
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APL-2 Inhibits C3, The Central Complement Protein
Lectin Pathway
C5aC3b
C5b MAC
Inflammation
Cell removal, Antigen uptake
by APCs
C5
C3a Inflammation
Classical Pathway
Alternative Pathway
APL-2
C3
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Cell death, secretion, lysis, or
proliferation
Central inhibition of complement
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Update From PLAUDIT: A Phase 2 Clinical Study of APL-2 for the Treatment of AIHA
Abstract Code: S899
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Randomized 1:1
Randomized 1:1
Key Eligibility Criteria
• Haemoglobin (Hb) levels <11 g/dL• Signs of haemolysis• Positive direct antiglobulin test (DAT) for IgG and/or complement C3
Study Design – Open Label
APL-2 Daily 270mg (N=6)
APL-2 Daily 360mg (N=6)
APL-2 Daily 270mg (N=6)
APL-2 Daily 360mg (N=6)
Cohort 1Warm AIHA
(N=12)
Cohort 2Cold AIHA
(N=12)
Efficacy assessed by change from baseline in
haematologic and blood chemistry parameters and
FACIT
Treat for 48 Weeks
Eligibility & Study Design
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Demographics and Baseline CharacteristicsCAD wAIHA C3+
270 mg(n=7)
360 mg(n=6)
All(n=13)
270 mg(n=3)
360 mg(n=5)
C3+(n=8)
Age, years, mean (SD) 68.9(8.5)
75.7(7.5)
72.0(8.5)
50.7(15.0)
51.6(23.6)
51.3(19.6)
Sex, n (%)MaleFemale
3 (42.9)4 (57.1)
1 (16.7)5 (83.3)
4 (30.8)9 (69.2)
0 (0.0)3 (100.0)
2 (40.0)3 (60.0)
2 (25.0)6 (75.0)
Ethnicity, n (%)Hispanic or LatinoNot Hispanic or Latino
1 (14.3)6 (85.7)
1 (16.7)5 (83.3)
2 (15.4)11 (84.6)
3 (100.0)0 (0.0)
3 (60.0) 2 (40.0)
6 (75.0)2 (25.0)
Race, n (%)WhiteNon-whiteOther
7 (100.0)0 (0.0)0 (0.0)
5 (83.3)1 (16.7)1 (16.7)
12 (92.3)1 (7.7)1 (7.7)
3 (100.0) 5 (100.0) 8 (100.0)
Weight, kg, mean (SD) 83.6(19.2)
71.0(10.6)
77.8(16.5)
70.4(7.0)
93.8(18.4)
85.1(18.8)
CAD= cold agglutinin disease; n=number of subjects; SD=standard deviation; wAIHA=warm antibody autoimmune hemolytic anemia9
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Results: Haematologic Parameters – All SubjectsBaseline Day 28 Day 168 Day 336
CAD Cohort: Mean (SD)Hb, g/dL 8.9 (0.4)
n=1311.2 (0.5)
n=1111.2 (0.6)
n=1012.6 (0.1)
n=2LDH, U/L 486.5 (142.1)
n=13267.1 (60.6)
n=12183.2 (22.2)
n=10155.5 (0.5)
n=2Bilirubin, mg/dL 1.9 (0.3)
n=130.7 (0.2)
n=120.4 (0.1)
n=100.7 (0.5)
n=2Haptoglobin, mg/dL 24.1 (10.2)
n=1347.7 (14.2)
n=1244.4 (13.8)
n=825.0 (15.0)
n=2ARC, 109/L 138.6 (18.5)
n=12101.5 (19.9)
n=1163.6 (6.0)
n=1079.7 (5.2)
n=2BMRI 94.0 (65.5)
n=1187.6 (63.5)
n=1148.7 (33.9)
n=1076.7 (8.7)
n=2wAIHA C3+ Cohort: Mean (SD)
Hb, g/dL 9.2 (0.3)n=8
10.5 (0.4)n=8
11.0 (0.7)n=5
11.0 ()n=1
LDH, U/L 337.4 (62.6)n=8
207.0 (22.1)n=7
142.2 (6.0)n=5
147.0 ()n=1
Bilirubin, mg/dL 0.8 (0.1)n=8
0.4 (0.1)n=7
0.3 (0.1)n=5
0.1 ()n=1
Haptoglobin, mg/dL 22.5 (8.3)n=8
66.1 (19.2)n=8
103.0 (30.6)n=5
195.0 ()n=1
ARC, 109/L 193.0 (21.8)n=8
112.7 (32.8)n=8
92.2 (19.3)n=5
42.0 ()n=1
BMRI 144.2 (48.1)n=8
93.2 (77.3)n=8
79.7 (32.1)n=5
38.5 ()n=1
ARC = absolute reticulocyte count; BMRI = bone marrow responsiveness index [(absolute reticulocyte count) X (subject’s Hb/normal Hb)]; CAD = cold agglutinin disease; Hb = hemoglobin; LDH = lactate dehydrogenase; n = number of subjects reporting data at this timepoint; SD = standard deviation; wAIHA = warm antibody autoimmune hemolytic anemia
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AIHA is A Puzzle!!
B-cellproliferation
Macrophagesystem
activation
Bone marrow
responseflogosis
Auto-immunity
Complement
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Reticulocytes in wAIHA and CAD in Response to APL-2
• Mean reticulocyte count decreased from 138.6´ 109/L at baseline to 63.6 ´ 109/L at day 168.
• Mean reticulocyte count decreased from 193.0 ´ 109/L at baseline to 92.2 ´ 109/L at day 168.
ARC in wAIHAARC in CAD
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Results: Transfusion and FACITTransfusions
FACIT Fatigue Score*
CAD wAIHA C3+Subjects with Transfusions (within 168 days before study entry) 6/13 2/8
Subjects with Transfusions(within 14 days on study) 2/13 0/8
Subjects with Transfusions(between 15-168 days on study)
2/13(both subjects discontinued
before day 168)
2/8(both subjects discontinued
before day 168)
Subjects at Day 168 with Transfusions after Day 14 0/10 2/5
CAD wAIHA C3+Mean (SD) Change from Baseline at Day 168
9.7 (2.3)n=10
2.4 (4.1)n=5
*FACIT- Clinically significant increase is 3 or more points
• CAD: Two heavily transfusion dependent patients did not respond to APL-2 and left the study at Day 56 and 108 respectively and one patient is still participating in the study and has not yet reached Day 168
• wAIHA C3+: Two of the eight enrolled C3+ wAIHA patients left the study due to lack of response prior to day 168; one of the eight enrolled C3+ wAIHA subjects is still participating in the study and has not yet reached Day 168
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Results: Safety
• All 13 CAD subjects and 10 of 11 wAIHA (C3+ and C3-) experienced ≥1 treatment-emergent adverse event (TEAE), mainly Grade 1-2.
• Ten subjects reported 13 unrelated serious adverse events (SAEs).
• Ten subjects experienced 26 unrelated/unlikely related Grade 3 TEAEs (haemolytic flare, pneumonia, dyspnoea, hypertension, acute cholecystitis, squamous cell carcinoma, purpura, hypokalaemia, acute kidney injury, dizziness, nausea, pain in extremity).
• 1 subject reported 2 unrelated Grade 4 TEAEs (high calcium, high creatinine) and withdrew.
• No grade 3 or 4 AEs were considered to be treatment related.
n = number of subjects with AEs in that category; m = number of AEs in that category.
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CategoryCAD
(n=13)wAIHA(n=11)
n (%) m n (%) m
Any TEAE 13 (100) 142 10 (90.9) 120
SAE 5 (38.5) 8 5 (45.5) 5
TEAEs Related to APL-2 7 (53.8) 26 8 (72.7) 25
TEAEs Leading to Discontinuation 2 (15.4) 3 2 (18.2) 4
Severe TEAEs (≥ Grade 3) 6 (46.2) 7 5 (45.5) 21
Treatment With APL-2 Has Been Well-Tolerated
Mainly grade 1-2
Grade 3-4 not related to APL-2Infections not related
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Summary
• APL-2 rapidly increases Hb values both in CAD and in wAIHA (C3+) within the first weeks of treatment
• APL-2 reduces both intra- and extravascular hemolysis, as shown by reductions in LDH, bilirubin and reticulocytes
• APL-2 effect seems linked to C3+ in wAIHA, but further testing in DATmono C3− subjects is required
• APL-2 appears to be well tolerated in patients with AIHA
Disposition
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CAD wAIHA C3+ Overall n(%)
DATmono C3- DATmono C3+
Screened Subjects 15 15 30
Enrolled Subjects 13 3 8 24
Study Drug Discontinuation 2 2 2 6
Did Not Complete Study 2 2 1 5
Early Withdrawal 2 2 1 5
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Thank you for the kind attention
Abstract Code: S899
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Results CAD: Haemoglobin ResponseRapid, Sustained and Durable Increase in Haemoglobin (Hb) in Response to APL-2
Normal range, 12-17.5 g/dL17
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Results CAD: Lactate Dehydrogenase (LDH) ResponseRapid, Sustained and Durable Decrease in LDH in Response to APL-2
Normal range, 87-252 U/L18
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Results CAD: ARC and Indirect Bilirubin Response
Normal range, 0.1-0.75 mg/dLNormal range, 30-100 ´ 109/L
Sustained and durable decrease in Absolute Reticulocyte Count (ARC) in Response to APL-2 Sustained and durable decrease in Indirect Bilirubin in Response to APL-2
• Mean reticulocyte count decreased from 138.6 ´109/L at baseline to 63.6 ´ 109/L at day 168.
• Mean indirect bilirubin decreased from 1.9 mg/dL at baseline to 0.4 mg/dL at day 168.
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Results wAIHA C3+: Haemoglobin and LDH Response
Normal range, 12-17.5 g/dL
• Mean haemoglobin (Hb) increased from 9.2 g/dL at baseline to 11.0 g/dL at day 168.
• Mean LDH decreased from 337.4 U/L at baseline to 142.2 U/L at day 168.
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APL-2improves anaemia (2 g/dL average)
Normalizes LDH
Increase in Hb in Response to APL-2 Decrease in LDH in Response to APL-2
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Results wAIHA C3+: ARC and Indirect Bilirubin Response
Normal range, 0.1-0.75 mg/dLNormal range, 30-100 ´ 109/L
Sustained and durable decrease in ARC in Response to APL-2 Sustained and durable decrease in Indirect Bilirubin in Response to APL-2
• Mean reticulocyte count decreased from 193.0 ´109/L at baseline to 92.2 ´ 109/L at day 168.
• Mean indirect bilirubin decreased from 0.8 mg/dL at baseline to 0.3 mg/dL at day 168.
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APL-2Normalizes ALL haemolytic markers…
Why is anaemia still there?