LaVision BioTec GmbHwww.lavisionbiotec.com

In Vivo Stabilization Package

Background

Sample motion due to heartbeat and breathing makes in vivo microscopy difficult leading to a blurred image or losing sight of the area of interest.

Benefits

The in vivo stabilization package prevents those movement artefacts that compromise your valuable data. Get the most out of your imaging in awake rodents using both software and hardware based motion stabilization.

LaVision BioTec’s Approach

LaVision BioTec’s in vivo stabilization package comes with:• Piezo driven Tripod moving

your objective in all dimensions

• Additional software based correction (x and y)

The Tripod is driven by innovative software algorithms calculating the movement of an object, e.g. a neuron, during data acquisition. The compensation runs up to 250 frames/s.

Highlights

• 3D motion correction• Stabilization feedback loop

based on direct analysis of the acquired data

• Calculation independent on framesize

• Accuracy < 50 nm• Response time < 2 ms• 3D feedback loop frequency

up to 500 Hz• Travel range Ø 40 µm (lateral)

and ±10 µm (axial)• Complete integration into

ImSpector with readout of actual and corrected position

• Compatible with resonant and galvanometric 2-Photon and wide-field microscopy

Three-dimensional motion stabilization in real time for awake rodents

LaVision BioTec GmbHAstastr. 1433617 Bielefeld, Germany

Tel: +49 (0) 521 915139-0 Fax: +49 (0) 521 915139-10info@lavisionbiotec.com

www.lavisionbiotec.com

Figure 1: 2-photon in vivo calcium imaging of an awake mouse using GCaMP as calcium indicator. Ca2+ signals were recorded with a reso-nant scanning microscope (TriM-Scope II, LaVision BioTec) while the mouse was running on a treadmill. The images are snapshots of two

superimposed sequences. Without motion stabilization (light blue), the neuron moves around the center of the screen. By using LaVision Bio-Tec’s in vivo stabilization package, lateral and axial shifts are compen-sated and the neuron is kept in the center over time (red).

Time

Application Example

Objective(not included)

Tripod

Spacer

M34x1 Screw Thread

15.0 mm (Minimum)*

24.5 mm

M25x0.75 Screw Thread**

* The spacer is also available in a height of 22.5 mm or can be built

according to the customer‘s specific requirements.

** The tripod is already designed for objectives with a M25x0.75 screw

thread (Olympus, Nikon) and can be fitted to most common objectives.

Ø 87.4 mm

USBD-Sub 37-poles

ElectronicBox PCTripod

Connections and Dimensions

The in vivo stabilization package comprises the tripod (including the spacer), an electronic box and a plug-in for ImSpector for direct control by PC.

Time [s]

Fluo

resc

ence

Sig

nal [

AU

]

Figure 2: Fluorescence signal over time with (red) and without (light blue) motion stabiliza-tion. By using LaVision BioTec’s in vivo stabi-lization package, the noise of the baseline can be minimized making Ca2+ signals detectable.

1500

2000

2500

3000

0 10 20 30 40

Ca2+ signal

ON ONOFF