immunotechnology

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LESSON PLAN LP- BT 1011

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SubCode & Name : 1011

IMMUNOTECHNOLOGY

Unit: I Branch: Biotechnology Semester : II

Course Objective and Scope:

Immunotechnology is a specialized course which deals with biotechnological applications. The immunological mechanisms are introduced in the earlier course BT- 1402 IMMUNOLOGY which deals on immune systems and their components at the cellular and humoral levels. Moreover, at the molecular level, focusing on gene rearrangement of immunoglobulin genes and T-cell receptor genes, and additionally, antigen processing and presentation, cellular responses, innate immunity and tolerance are included in that course. Knowledge on the fundamentals of immunology forms the basis of the course immunotechnology which deals with the practical aspects such as the production and engineering of antibodies, the application of antigens, the design of (recombinant) vaccines, strategies for immune intervention, etc.

The Immunotechnology-based techniques will be used in the medicine for therapeutics and diagnostics, industries in the production, quality control and quality assurance, and in R&D

Learning outcomes:

After this course, the students will be able to:

- understand the techniques like immunodiagnostic tests, characterization of lymphocytes, purification of antigens, antibody engineering, etc.

- assess health problems with immunological background;

- develop approaches for the immune intervention of diseases;

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UNIT I: ANTIGENS

Syllabus: Types of antigens, their structure, preparation of antigens for raising antibodies, handling of animals, adjuvants and their mode of action.

Objectives: 1.To explain the different types of antigen and their structure 2. To describe the preparation of antigens for raising the antibodies and explain the role of adjuvants and their mode of action. 3. To explain the different methods of handling of animals for immunological studies

SessionNo Topics to be covered Time

(min)

TB/RF

Teaching Aids

1. Types of antigens and their structure 50 TB1/TB2 LCD/BB/OHP

2. Preparation of antigens for raising antibodies;adjuvants and their mode of action

50 TB1/TB2 LCD BB/OHP

3. Handling of animals 50 TB1/TB2 LCD BB/OHP


LP Rev. No: 00

Date: 27/06/11

of 8

SubCode & Name : BT 1011

IMMUNOTECHNOLOGY

Unit: I Branch: Biotechnology Semester : VIII

DOC/LP/01/28.02.02


LP Rev. No: 00

Date: 27/06/11

of 8


IMMUNOTECHNOLOGY

Unit: II Branch: Biotechnology Semester : VIII

UNIT II: ANTIBODIES & IMMUNODIAGNOSIS

Syllabus: Monoclonal and polyclonal antibodies – their production and characterization, western blot analysis, immuno electrophoresis, SDS-PAGE, purification and synthesis of antigens, ELISA-principle and applications, radio immuno assay (RIA) principles and applications, non isotopic methods of detection of antigens-enhanced chemilluminescence assay

Objectives: 1. To make the student to learn the basis of the production and characterization of monoclonal antibodies 2. To make the student to learn the basis of the production and characterization of polyclonal antibodies. 3. To understand the techniques western blot analysis, immuno electrophoresis, SDS-PAGE ELISA-principle and applications, radio immuno assay and chemilluminescence assay

SessionNo Topics to be covered Time TB/

RFTeaching Aids

1 Polyclonal antibodies; their production and characterization

50 TB1/TB2

LCD/ BB/OHP

2 Monoclonal antibodies; their production and characterization –I

50 TB1/TB2

LCD/ BB/OHP

3 Monoclonal antibodies; their production and characterization – II

50 TB1/TB2

LCD /BB/OHP

4 SDS-PAGE; Western blot analysis 50 TB1/TB2

LCD /BB/OHP

5 Immuno-electrophoresis 50 TB1/TB2

LCD /BB/OHP

6 Purification and synthesis of antigens 50 TB1/TB2

LCD /BB/OHP

7 ELISA - principle and applications 50 TB1/TB2

LCD/ BB/OHP

8 Radio immuno assay (RIA) principles and applications

50 TB1/TB2

LCD/ BB/OHP

9 Non-isotopic methods of detection of antigens-enhanced chem. iluminescence assay

50 TB1/TB2

LCD/ BB/OHP


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IMMUNOTECHNOLOGY

Unit: III Branch: Biotechnology Semester : VIII

UNIT III: ASSESMENT OF CELL MEDIATED IMMUNITYSyllabus: Identification of lymphocytes and their subsets in blood. T cell activation parameters, estimation of cytokines, macrophage activation, macrophage microbicidal assays, in-vitro experimentation-application of the above technology to understand the pathogenesis of infectious diseases.

Objectives: To make the students: 1 to understand the methods for identification of lymphocytes and their subsets in blood 2. To understand the mechanism of T cell activation 3. To understand the methods of estimation of cytokines 4. To understand the mechanism of macrophage activation 5. To understand the macrophage micobicidal assays used to understand the pathogenesis of different infectious diseases.

SessionNo Topics to be covered Time

TB/RF

Teaching Aids

1 Identification of lymphocytes and their subsets in blood – I

50 TB1/TB2

LCD/ BB/OHP

2 Identification of lymphocytes and their subsets in blood – II

50 TB1/TB2

LCD/ BB/OHP

3 Identification of lymphocytes and their subsets in blood – III

50 TB1/TB2

LCD/ BB/OHP

4 T cell activation parameters I 50 TB1/TB2

LCD/ BB/OHP

5 T cell activation parameters II 50 TB1/TB2

LCD/ BB/OHP

6 Estimation of cytokines – I 50 TB1/TB2

LCD/ BB/OHP

7 Estimation of cytokines – II 50 TB1/ TB2

LCD/ BB/OHP

8 Macrophage activation- I 50 TB1/TB2

LCD/ BB/OHP

9 Macrophage microbicidal assays – I

50 TB1/ TB2

LCD/ LCD/ LCD/ BB/OHP

10 Macrophage microbicidal assays – II

50 TB1/ TB2

LCD/ BB/OHP

11 Macrophage microbicidal assays - III

50 TB1/TB2

LCD/ BB/OHP

12 Macrophage microbicidal assays – IV

50 TB1/ TB2

LCD/ BB/OHP

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Date: 27/06/11

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IMMUNOTECHNOLOGY

Unit: IV Branch: Biotechnology Semester : VIII

UNIT IV: IMMUNOPATHOLOGY

Syllabus: Preparation and storage of tissues, identification of various cell types and antigens in tissues, isolation and characterization of cell types from inflammatory sites and infected tissues, functional studies on isolated cells, immuno cytochemistry – immuno fluorescence, immuno-enzymatic and immuno-ferrtin techniques, immuno-electron microscopy

Objectives: To make the students to: 1. Understand the preparation and storage of tissues for immunological identification 2. understand the methods for identification of various cell types and antigens in tissues 3. Understand the isolation and characterization of cell types from inflammatory sites and infected tissues 4. to understand the methods for functional studies on isolated cells 5. to learn the techniques of immnocytochemistry


RF

Teaching Aids

1 Preparation and storage of tissues 50 TB1/ TB2

LCD/ BB/OHP

2 Identification of various cell types and antigens in tissues

50 TB1/TB2

LCD/ BB/OHP

3 Isolation and characterization of cell types from inflammatory sites and infected tissues - functional studies on isolated cells

50 TB1/TB2

LCD/ BB/OHP

4 Immuno cytochemistry immuno fluoresecence 50 TB1/TB2

LCD/ BB/OHP

5 Immuno enzymatic and immuno ferrtin techniques 50 TB1/TB2

LCD/ BB/OHP

6 Immuno electron microscopy 50 TB1/TB2

LCD/ BB/OHP


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IMMUNOTECHNOLOGY

Unit: V Branch: Biotechnology Semester : VIII

UNIT V: MOLECULAR IMMUNOLOGY

Syllabus: Preparation of vaccines, application of recombinant DNA technology for the study of the immune system, production of antidiotypic antibodies, catalytic antibodies, application of PCR technology to produce antibodies and other immunological reagents, immuno therapy with genetically engineered antibodies.

Objectives: Make the students 1. to understand the methods of preparation of vaccines 2. Understand the basis and applications of rDNA technology for study of immune system 3. To learn basis and the methods for production of antidiotypic antibodies and catalytic antibodies 4. to understand the the applications of PCR technology 5. to learn about immunotherapy


RF

Teaching Aids

1 Preparation of vaccines - I 50 RF1 LCD/ BB/OHP

2 Preparation of vaccines - II 50 RF1 LCD/ BB/OHP

3 Preparation of vaccines - III 50 RF1 LCD/ BB/OHP

4 Application of recombinant DNA technology for the study of the immune system – I

50 RF1 LCD/ BB/OHP

5 Application of recombinant DNA technology for the study of the immune system – II

50 RF1 LCD/ BB/OHP

6 Application of recombinant DNA technology for the study of the immune system – III

50 RF1 LCD/ BB/OHP

7 Production of anti-idiotypic antibodies, catalytic antibodies

50 RF1 LCD/ BB/OHP

8 Application of PCR technology to produce antibodies and other immunological reagents

50 RF1 LCD/ BB/OHP

9 Immunotherapy with genetically engineered antibodies.

50 RF1 LCD/ BB/OHP

UNIT VI: CURRENT TOPICS IN IMMUNOLOGY

Syllabus: Trends in Immunology of infectious diseases and tumors.

Objectives: To make the students 1. to understand the recent trends in immunology of infectious diseases 2. to understand the recent trends in immunology of cancer biology


RF

Teaching Aids

1 Trends in Immunology of Infectious diseases -I 50 RF1 LCD/ BB/OHP

2 Trends in Immunology of Infectious diseases- II 50 RF1 LCD/ BB/OHP

3 Trends in Immunology of Infectious diseases- III 50 RF1 LCD/ 4 Trends in Immunology of Infectious diseases- IV 50 RF1 LCD/

BB/OHP

5 Trends in Immunology of Tumors – I 50 RF1 LCD/ BB/OHP

6 Trends in Immunology of Tumors – II 50 RF1 LCD/ BB/OHP

Course Delivery Plan

Week1 2 3 4 5 6 7 8 9 10 11 12 13 14 15

Units I II III IV V VI

Text Books (TB):

1. Talwar G.P., and Gupta S.K., “A hand book of practical and clinical immunology”, Vol. 1 & 2, CBS Publications, 1992.

2. Weir D.M., “Practical Immunology”, Blackwell Scientific Publications, Oxford, 1990.

References (RF):

1. Austin J.M. and Wood K.J., “Principles of cellular and molecular immunology”, Oxford university press, Oxford, 1993.

Prepared by Approved bySignature

Name Prof. M. Sivanandham Prof. M. SivanandhamDesignation Professor HOD of BiotechnologyDate June 28, 2011 June 28, 2011

immunotechnology

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