I Zapolsky, E Kyzar, S Gaikwad, A Roth, J Green, M Pham, PG St. Pierre, B Hirons, AV Kalueff

(New Orleans, USA)

ZEBRAFISH NEUROPHENOME PROJECT

The ZNP was started by the ZNRC, and is dedicated toward producing a ZND

Let’s start by defining these acronyms

The ZNRCZebrafish Neuroscience Research Consortium

Allan Kalueff - USA Georgianna Gould - USA Oliver Braubach - Canada Anderson Manoel Herculano - Brazil David Echevarria - USA Joe Schroeder - USA Jason Warnick - USA Dominic Wright - SwedenCarla Denise Bonan - BrazilMônica Ryff Moreira Roca Vianna - BrazilZofia Zukowska - USA

The main goal of ZNRC is to promote zebrafish neuroscience research.

Created in February 2010, ZNRC offers excellent networking opportunities and peer support for active zebrafish labs.

ZNDZebrafish Neurophenome Database

Goals:PioneeringAccessibleEasy to useUseful

Emerging Model Organism

The Zebrafish is an emerging model animal for vertebrate development and neurological function

Offers a good compliment to other animal models

Advantages Easy to take care of Inexpensive Easy to test Quantifiable behavioral endpoints

Inspiration

MiceMouse Genome

Informatics (G+P)Mouse Phenome

Database (P)

RatsRat Genome Database

(G)National BioResource

Project (P)

ZebrafishZFIN (Genome)

NCBI (Genome)

The point?

A Phenome database for the Zebrafish does not yet exist, but inevitably will sometime soon

PubMed search“zebrafish”

1900-1985 - 61 papers

1985-1990 - 114 papers

1990-1995 - 487 papers

1995-2000 - 1981 papers

2000-2005 - 4946 papers

2005-2010 - 7538 papers

Between 2005 and 2010 the total number of zebrafish-related papers ever written doubled.

ZNDZebrafish Neurophenome Database

Goals:Pioneering (so far)AccessibleEasy to useUseful

www.kaluefflab.com/znpindex-or-

www.tulane.edu/~znpindex/search

ZNDZebrafish Neurophenome Database

Goals:Pioneering (so far)Accessible (the internet)Easy to useUseful

What we see, Not easy

What you’ll see

What you’ll see

What you’ll see

Everything you need to know… Hopefully

If not..

Submit your data! More on this later

How it’s organized

Experiment 1

Interval 1.1

Treatment 1.1.1

Interval 1.2

Interval 1.3

Treatment 1.1.2

Parent/Child Format

Caffeine L/D Box

15 min

100 mg/kg I.P.

250 mg/kg I.P.

Chronic

Acute withdraw

al

Experiment

Interval

Treatment

that’s what you’re seeing here

Experiment

IntervalTreatment

TestResults

ZNDZebrafish Neurophenome Database

Goals:Pioneering (so far)Accessible (the internet)Easy to use (we hope so) Useful

Only With Your Help

Submit your data! (2 methods)1.Direct info submission with the

submission template (next slide)2.Send us a paper (please use step

#1)

Submission Template

If you send us a paper…

We fill out the template

ZNDZebrafish Neurophenome Database

Goals:Pioneering (so far)Accessible (the internet)Easy to use (we hope so) Useful (with your help)

Special Thanks

Budd Hirons - who has been essential to the actualization of this idea.

ILC (Innovative Learning Center) & Tulane University Technology Services

Also, Tulane has been kind enough to supply us with the server space that is required to host such a database.

In Conclusion

The Zebrafish is an emerging animal model for vertebrate development and neurological function

We hope to work closely with other zebrafish-based databases to help provide a better, more comprehensive user experience

Check in regularly at: http://www.tulane.edu/~znpindex/search Or just search ZNP or Zebrafish Phenome

You can help by submitting your data.