How do cells acquire energy? Why do cells need ib. Is the Cells Energy Currency ATP (a nucleotide) ATP is used in numerous biological processes. Examples: provides energy for heat, nerve electricity, light (fireflies), muscle movement, pumping substances across ...
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Todays OutlineMetabolismEnergy-Releasing PathwaysAerobic Respiration GlycolysisKrebs CycleElectron Transport PhosphorylationAnaerobic PathwaysEnergy and WorkATPMetabolic PathwaysEnzymesFeaturesFactors Affecting Enzyme ActivityMembrane TransportDiffusionOsmosisPassive TransportActive TransportBulk TransportThe cells capacity to:1. Acquire energy.2. Use energy to build, degrade, store and release substance in a controlled manner.MetabolismHow do cells acquire energy?By breaking down high energy molecules in or food.For example: when we eat carbohydrates:1. Digestion breaks these complex sugars down to glucose. 2. Glucose, a high energy molecule, is absorbed across the gut into your bloodstream. 3. An increase in blood glucose triggers the pancreas to release insulin.4. Insulin signals cells to start taking up more glucose.5. Glucose in the cell is the beginning of metabolism.Why do cells need energy?Cells need energy to do work.Type ExamplesChemical building, rearranging,breaking apart substancesMechanical moving flagella, cell structures,parts of or the whole bodyElectrochemical moving charged substances acrossmembranesMetabolism1. To further understand how metabolism works we must introduce some concepts, processes and participants.Concepts & Processes1. Energy2. Gradients 3. Phosphorylation4. Membrane transport 5. Metabolic pathways6. Aerobic respiration7. Anaerobic pathwaysParticipants1. Glucose2. Adenosine triphosphate (ATP)3. Enzymes4. Cofactors5. MitochondriaEnergyEnergy is the capacity to do work and cant be created from nothing.Where does energy come from?Mostly from the sunEnergyTHERMODYNAMICS:1st Law: Any isolated system has a finite amount of energy that cannot be added to or lost, but can be converted from one form to another.(conservation of energy)2nd Law: Systems move from more ordered to less ordered. (entropy)EnergyEnergy flows in one direction.Sun Photosynthetic producers consumersHEATEnergyEnergy is stored and released by building and degrading molecules (energy is stored in chemical bonds).1. Endergonic reactions Energy in.2. Exergonic reactions Energy out.EnergyENERGY INenergy-poor starting substances6 12GlucoseEndergonic reactionsare required to produce energy-rich compounds like glucose (photosynthesis). Energy must be added to the reaction to convert low-energy molecules into high energy molecules.EnergyENERGY OUTenergy-poor products6+6 O2GlucoseExergonic reactionsResult in the net release of energy (aerobic respiration). Molecular bonds are broken in a step by step process so that cells can capture energy in a controlled fashion.6ATP Is the Cells Energy Currency ATP(a nucleotide)ATP is used in numerous biological processes.Examples: provides energy for heat, nerve electricity, light (fireflies), muscle movement, pumping substances across membranes against a gradient.Metabolic PathwaysMost metabolic reactions occur in orderly, enzyme-mediated sequences. These are metabolic pathways.Metabolic reactions start with reactants.Intermediates are formed during the reaction.The substances at the end are known as products.Many metabolic pathways are reversible with products being converted back to reactants. Reversible reaction help maintain an equilibrium.Features of Enzymes1. Nearly all enzymes are proteins.2. Speed rate of reaction by lowering activation energy.Enzymes Lower Activation Energyactivation energywithout enzymeactivation energywith enzymeenergyreleasedby thereactionproductsstarting substancedirection of reactionFigure 5.8 from page 78 of your textFeatures of Enzymes (Cont.)3. Enzymes are not used up or permanently altered.4. Enzymes are substrate specific.Factors Affecting Enzyme Activity1. Temperature2. pH3. SalinityActual ranges of activities differ among enzymes. Figures 5.13 &.14 from page 81 of your text.Crossing Membranes1. Cell membranes are selectively permeable.2. CO2, O2, and small nonpolar molecules pass through the membrane.3. Polar water molecules slip though gaps in the cell membrane when the lipid bilayerflexes and bends.4. Ions and large polar molecules such as glucose must pass through transport proteins in cell membrane.OsmosisOsmosis diffusion of water molecules in a concentration gradient across a selectively permeable membrane.Water moves in the direction necessary to equalize the concentrations.Passive and Active Transport1. In both processes, solutes move through transport proteins in the cell membrane.2. In passive transport, substances move passively (they diffuse).3. In active transport, ATP is required to pump substances against a concentration gradient. Passive and Active Transport AnimationsPassive Transport:Solutes follow gradientsActive Transport:Solutes move againstgradientsBulk Transport1. Cells use vesicles to expel or take in large items or large numbers of items.2. Exocytosis vesicle fuses with plasma membrane and contents are released outside cell.3. Endocytosis cell surrounds items at outer surface and brings them inside, creating a vesicle.Endocytosis and ExocytosisAnimationMetabolism1. All cells make ATP by pathways that release chemical energy from organic compounds such as glucose.2. Cells store chemical energy as ATP to use in future reactions that require energy input. MetabolismThere are two pathways for generating ATPfrom glucose:Aerobic Respiration requires O2Anaerobic Respiration no O2 neededAerobic RespirationThree steps:1. Glycolysis In Cytoplasm2. Krebs Cycle In Mitochondria3. Electron Transport Phosphorylation - MitochondriaGlycolysis MovieOverview of Aerobic RespirationCYTOPLASMMITOCHONDRIONGLYCOLYSISELECTRON TRANSPORT PHOSPHORYLATIONKREBS CYCLE ATPATPenergy input to start reactions2 CO24 CO2232water2 NADH8 NADH2 FADH22 NADH 2 pyruvatee- + H+e- + oxygen(2 ATP net)glucoseTYPICAL ENERGY YIELD: 36 ATPe-e- + H+e- + H+ATPH+e- + H+Figure 7.3 from page 111 of your textAerobic Respiration OverviewAnimation Electron Transport Animation Fig. 7.9, p. 115ATPATPATPATP36 ATP28422ATPATPglucose2 PGAL2 pyruvate2NAD+2 NADH2 FADH22 NADH2 FADH26 NADH2 acetyl-CoAKREBSCYCLEATPATPATPATP2 CO2cytoplasmADP + PiELECTRON TRANSPORT PHOSPHORYLATIONGLYCOLYSISinner mitochondrial compartmentouter mitochondrial compartmentElectrons and hydrogen from cytoplasmicNADH are shuttled into inner compartment. Two coenzymes already inside transfer the electrons to a transport system.Coenzymes (8 NAD+, 2 FAD total) transfer electrons and hydrogen from remnants of pyruvate to a transfer system.Electrons flow through transport systemTransport system pumps H+to the outer compartmentAt ATP synthases H+ flowing back in drives ATP formation2 NADH for in the cytoplasmd In third stage, NADHand FADH2 from second stage used to make 28 ATP by electron transport Phosphorylation.c In third stage, NADH from glycolysis used to form 4 ATP by electron transport phosphorylation.b In Krebs cycle of second stage, 2 ATP form by substrate-level phosphorylation.a In glycolysis, 2 ATP used; 4 ATP form by substrate-level phosphorylation. So net yield is 2 ATP.NADHFig. 7.8a, p. 114OUTER COMPARTMENTINNER COMPARTMENTATPADP+PiFig. 7.8b, p. 114INNER COMPARTMENTAnaerobic Respiration Pathways1. Bacteria and yeast use anaerobic respiration. Their metabolic pathways do not use oxygen as the final acceptor of electrons that ultimately form ATP.2. In both lactate fermentation and alcoholic fermentation, only 2 ATP are produced from each glucose molecule.
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Energy Transfer. ATP zEnergy Currency zPotential energy in ATP used for all energy requiring processes of cells.
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