histology review eye and ear dr. tim ballard department of biology and marine biology

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HISTOLOGY REVIEW Eye and Ear Dr. Tim Ballard Department of Biology and Marine Biology

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Page 1: HISTOLOGY REVIEW Eye and Ear Dr. Tim Ballard Department of Biology and Marine Biology

HISTOLOGY REVIEWEye and Ear

Dr. Tim BallardDepartment of Biology and Marine Biology

Page 2: HISTOLOGY REVIEW Eye and Ear Dr. Tim Ballard Department of Biology and Marine Biology

The dashed lines indicate the locations of the suspensory ligaments connecting the ciliary processes to the capsule around the perimeter of the lens.

Structures of the eyeStructures of the eye

Eyeball (anterior) – section – H&E – 4x objective

cornea

ciliary processe

s

anterior chamber

lens

posterior chamber

posterior cavityciliary

body

conjunctiva

iris

Page 3: HISTOLOGY REVIEW Eye and Ear Dr. Tim Ballard Department of Biology and Marine Biology

The dashed line indicates the location of the pupil, located between the anterior-most margins of the iris.

Structures of the eyeStructures of the eye

Eyeball (anterior) – section – H&E – 4x objective

cornea

anterior chamber

lens

conjunctiva

iris

pupil

Page 4: HISTOLOGY REVIEW Eye and Ear Dr. Tim Ballard Department of Biology and Marine Biology

The brown-black pigments of the choroid are melanin granules. Go back to the previous two slides and follow this pigment into the iris.

Structures of the eyeStructures of the eye

Eyeball (posterior) – section – H&E – 10x objective

choroid

posterior cavity

retina

sclera

Page 5: HISTOLOGY REVIEW Eye and Ear Dr. Tim Ballard Department of Biology and Marine Biology

Plexiform layers are areas of synapses between adjacent neuronal types.

Structures of the eyeStructures of the eye

Retina – section – H&E – 40x objective

outer nuclear layer

optic nerve fibers

choroidpigmented epithelium

ganglion cell layer

layer of rods and cones

outer plexiform layer

inner nuclear layer

inner plexiform

layer

Page 6: HISTOLOGY REVIEW Eye and Ear Dr. Tim Ballard Department of Biology and Marine Biology

Cochlea – longitudinal section – H&E – 4x objective

The cochlea is a coiled structure, turning 2.75 times about a central axis (the modiolus). So, you see the same tube multiple times in the same structure.

Structures of the earStructures of the ear

bony cochlea

vestibule

modiolus

petrous portion of the temporal

bone

Page 7: HISTOLOGY REVIEW Eye and Ear Dr. Tim Ballard Department of Biology and Marine Biology

Cochlea – longitudinal section – H&E – 10x objective

During histological processing the delicate tectorial membrane breaks free from its attachment and curls back on itself.

Structures of the earStructures of the ear

spiral ganglio

n

scala tympani

basilar membrane

spiral organ of

Corti

vestibular membrane

scala media (cochlear duct)

scala vestibuli

tectorial membrane (torn free)

Page 8: HISTOLOGY REVIEW Eye and Ear Dr. Tim Ballard Department of Biology and Marine Biology

Cochlea – longitudinal section – H&E – 40x objective

The spiral organ consists of hair cells, the stereocilia of which are embedded in the overlying tectorial membrane, and support cells.

Structures of the earStructures of the ear

scala tympani

basilar membrane

spiral organ of

Corti

vestibular membran

e

scala media (cochlear duct)

scala vestibuli

tectorial membrane

end