grm 2013: improving phosphorus efficiency in sorghum by the identification and validation of sorghum...
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These slides are a redacted (by the presenter) version of what was presented at the meetingTRANSCRIPT
Improving Phosphorus Efficiency in Sorghum by the Identification and Validation of Sorghum Homologs for Pup1, a Major QTL Underlying
Phosphorus Uptake in Rice (SorghumPup1)
ICRISAT - Mali Eva Weltzien, Fred Rattunde, Willmar Leiser
IRRI/JIRCAS Sigrid Heuer (IRRI) Rico Gamuyao (IRRI)
Matthias Wissuwa (JIRCAS)
GCP – GRM 2013, Lisbon - Portugal
Moi Unversity Sam Gudu
INRAN - Niger Soumana Souley
Cornell University/USDA-ARS Leon Kochian (USDA/CU) Randy T Clark (USDA/CU) Brittany Green (USDA/CU)
Jon Shaff (USDA/CU) Sharon Mitchell (IGD/CU)
Sylvia de Sousa Robert Schaffert Claudia Guimarães
Lidiane Braga (PhD S) Gabriel Corradi (PhD S)
Barbara Negri (Und) Fabiano Matos (Und)
ESALQ/USP Antonio Augusto F. Garcia
Rodrigo Gazaffi
ICRISAT - Niamey Bettina Haussmann and Tom Hash
Jurandir Magalhaes Barbara H Maciel (PhD student)
A Molecular Tool Kit for Highly Weathered Tropical Soils
Al toxicity
High P fixation
Low activity clay
Variable charge
> 70 % Kaolinate/ Fe and Al oxidesEquator
Capricorn
Low P soils Acid soils (Al tox)
NHCOOH
Cytoplasm
3
*
AltSB Pstol1
Plant Phys 156-1041
Fe - O
Citrate
PO4--
Band A
Band B
Band C
10 ppm (P)
5 ppm (P)
0 ppm (P)
10 ppm (P)
5 ppm (P)
0 ppm (P)0 - 20 cm
- Spatial models (r/c) - Environmental covariates
- Phenological covariates
Phenotyping for Phosphorus Efficiency
- Soil moisture strongly impaires P diffusion
- PAE x PUTIL
Phenotyping for Root Morphology (2D)
- Importance for PAE - Shallow roots?
PSTOL1 increases grain yield
PSTOL1 increases early root growth
Comparative Strategy to Validate Pstol1 Homologs as Genes Conferring Enhanced P acquisiton
Sorghum: association mapping US (converted) Panel
Contrasting parental lines Pstol1 - Rice
Maize: QTL mapping L22
Grain yield: low P soils
Root morphology: nutrient solution
Wide Diversity Panel
Parentoni et al. (2010) Maydica
Polymorphism Discovery for Linkage and Association Mapping
• Primer design for amplicon sequencing • SNP detection • Conversion to the Kaspar system • Genotyping in the association panel
Primer-BLAST Conserved Regions
Paralog-specific primers
Step 2: Producing F1: Ms3ms3
Step 3: Self-pollinate F1s
Step 4: Random Matting Cycle 1
Step 5: Random Matting Cycle 2
Step 1: Base population development
Step 6: - Random Matting Cycle 3 - Pup1/AltSB validation
Selection for AltSB based on allele mining with tag markers
Genotyping
Genotyping S1s to produce S2s
Phenotype S2s and relate genotypic information on AltSB/Pstol1 with the phenotype for validation
- Provides means to validate Pup1/AltSB (higher LD) - Effect estimate - Amenable to gene pyramidation - Contingency plan based on a familiar breeding scheme (high risk project) - Entry points for new material (SorghumCI) - Cultivar release, regardless
Sorghum RMPs: pyramiding AltSB and multiple Pstol1 homologs
4 cycles of random matting
Products: - Agronomic performance under low P (SAP) - Root morphology and architecture (SAP) - Root morphology QTL - Functional markers