gregory schneider graphene bionanoscience:...
TRANSCRIPT
Gregory SCHNEIDER Graphene bionanoscience: from hydrophobicity to DNA sequencing
PhD at the University of Strasbourg,with Prof. Gero Decher, on nanoparticle
functionalisation using the Layer-by-layer technique with applications as drug
delivery vehicles for cancer therapy.
From 2006-2009, postdoc with Prof. George Whitesides at the Dept of
Chemistry and Chemical Biology at Harvard University on protein biophysics
and microfluidic devices
Currently senior postdoc with Prof. Cees Dekker at the Kavli Institute of
Nanoscience at TU Delft on graphene self-assembly and graphene nanopore
devices for genomic DNA sequencing.
He is looking forward to starting his own group at the frontier of rational
bioorganic self-assembly, microfluidics and graphene bionanoscience.
Graphene Bionanoscience From Hydrophobicity to Genomic DNA Sequencing
G.F. Schneider, [email protected] Cees Dekker group, Kavli Institute of Nanoscience, TU Delft, NL
Solid state nanopores
-Principle and drawbacks
Hydrophobic on hydrophilic
-Graphene wedging
Graphene nanopores
-Towards DNA Sequencing
Outline of this talk
DNA translocation through a nanopore Idea of the experiment:
For a review see: C. Dekker, Nature Nanotech. 2, 209 (2007)
Dreams of nanopore-based DNA sequencing
Advantages of nanopore sequencing:
• single molecule • no amplification • label-free • long read lengths • electrical detection
Solid-state nanopores from silicon processing
5 nm
A. Storm et al, Nature Mater. 2, 537 (2003)
ssDNA/RNA through α-hemolysin membrane pores (Kasianowicz, Branton, Akeson, Bayley, Meller, …)
Blockades of polyU through α-hemolysin
A major drawback for sequencing: the thickness
30 nm thick SiN 0.3 nm/base
� Average of 100 bases
Genomic screening beyond mere DNA sequencing Scanning the position of proteins along DNA
Screening of local proteins along DNA, e.g., transcription factors, nucleosomes..
Use SINGLE-ATOM-LAYER-THIN graphene as the membrane for a nanopore
Postma, Nanoletters 2010
Graphene by mechanical exfoliation
Blake et al, APL, (2007)
Hydrophobic polymer
Hydrophilic substrate
‘Wedging transfer’
Schneider et al, Nano Letters 10, 1912, April 19, 2010
A few flakes The target
After transfer Polymer dissolved
Using wedging to transfer graphene
10 μ
Schneider et al, Nano Letters 10, 1912, April 19, 2010
10 μ 10 μ
Transfer of a graphene monolayer by wedging
Schneider et al, Nano Letters 10, 3163, July 7, 2010
Drill a nanopore with a focussed TEM beam
Schneider et al, Nano Letters 10, 3163, July 7, 2010
Translocation of dsDNA through graphene nanopores
Schneider et al, Nano Letters 10, 3163, July 7, 2010
Scatter diagram conductance blockade versus translocation time
Schneider et al, Nano Letters 10, 3163, July 7, 2010
Very recent work: Towards atomically precise graphene nanopores, using self repair at high temperature
Song, Schneider et al, submitted
Summing-up:
� Solid-state and biological nanopores are thick
� Wedging allows minute-graphene transfer
� DNA translocate through pores in graphene
� Self-repair permits graphene with crystaline edges
� Current tunneling is next
Our group:
Stefan Kowalczyk Vlad Karas Koen Schuurbiers Kasper van Schie Okke Groen Stephanie Luik Xuyi Wang Cees Dekker
Collaborations:
Henny Zandbergen Bo Song Qiang Xu Gregory Pandraud Meng Yue Wu
Lieven Vandersypen Victor Calado Gilles Buchs