Glycolysis and Krebs' Cycle

Grade:«grade»Subject:«subject»

Date:«date»

1 Which of the following enzymes catalyzes a reaction that produces ATP during glycolysis?

A Hexokinase (HK)

B Phosphoglucose isomerase (PGI)

C Phosphofructokinase (PFK)

D Triosephophate isomerase (TPI)

E Pyruvate Kinase (PK)

2 Which of the following enzymes breaks one 6-carbon sugar into two 3-carbon sugars?

A Hexokinase (HK)

B Phosphoglucoisomerase (PGI)

C Phosphofructokinase (PFK)

D Aldolase

E Enolase

3 If a person would be deficient in all three forms of PFK, this person would ___ .

A Have lower than normal glycogen stores .

B Have more mitochondrial activity than normal .

C Be dead .

DProduce more energy per glucose molecule because glycolysis would be unregulated .

4 Assuming that Dr. Saccharo is correctly assuming that Ann has a defect in glycolysis, what is the most likely defect based on the blood metabolic profile?

A HexokinaseB Phosphofructokinase

C Triosephosphate IsomeraseD Pyruvate kinase

E Aldolase

5 The muscle biopsy showed an excess of glycogen in the muscle tissues. What is the most likely enzyme deficiency?

A Pyruvate kinaseB Hexokinase

C PhosphofructokinaseD Triosephosphate Isomerase

E Aldolase

6 If you wanted to test red blood cells for their ability to complete glycolysis, what compound would you try to detect?

A Fructose-6-phosphateB Aldolase

C Pyruvate

D Phosphofructokinase

E Dihydroxyacetone phosphate

7 If you had some of Marie's red blood cells in a test tube, what compound could you add to enable these cells to produce pyruvate?

A Glucose

B Fructose-1,6,bisphosphate

C Insulin

D Glucagon

E Fructose-6-Phosphate

8 Identify the substate present at step #8

9 Identify the product of step #9

10 Identify the reactant at step #10

11 Identify the product of step #11

12 State the total number of ATP produced by substrate-level phosphorylation for each pyruvate molecule that enters the mitochondrion.

13 State the total number of NADH molecules that are produced per molecule of glucose by the end of the

Krebs cycle.