GETTING THE MOST OUT OF YOUR DIETS:OPTIMAL FORMULATION WITH DSM ENZYMES

BRETT ROOSENDAAL 20 NOVEMBER 2013

RAPID ADOPTION

OBJECTIVEOBJECTIVEIleal Protein Dig.Coeff.

AME (MJ/kg DM) Starch Dig.Coeff.

CONTROL 0.78bc 14.07e 0.75c

XYLANASE 0.77c 14.37d 0.83b

PHYTASE 0.81ab 14.62c 0.86ab

PROTEASE 0.82a 14.81bc 0.83b

XYL + PHY 0.81abc 14.75c 0.89ab

XYL + PROT 0.80abc 14.66c 0.86ab

PHY + PROT 0.83a 14.99ab 0.88a

XYL + PROT + PHY 0.81ab 15.18a 0.87a

SEM 0.01 0.06 0.01Sultan, A., Li, X., Zhang, D., Cadogan, D.J. and Bryden, W.L. (2010) Dietary enzymes alter sorghumProtein digestibility and AME content. APSS 21Sultan, A., Gan, C.Y., Li, X., Zhang, D. and Bryden, W.L. (2011) Dietary enzyme combinations improve sorghum ileal protein and starch digestibility during the broiler starter phase. APSS 22

PHILOSOPHY ON ENZYMESPHILOSOPHY ON ENZYMES

• IMPROVE DIGESTION OF UNDIGESTED SUBSTRATES• ENZYMES ARE CHOSEN ON THE BASIS OF SUBSTRATES

• IMPROVED BIRD PERFORMANCE (ADG, FCR, MORTS)THROUGH MAXIMISING NUTRIENT UTILIZATION

• IMPROVED PROFITABILITY

EXPECTATION

• SUPERDOSING OF PHYTASE?

• CORN-SOYA DIETS – 85 TO 95% OF STARCH, PROTEIN AND LIPIDS ARE DIGESTED

• POORLY DIGESTED INGREDIENTS AS LOW AS 75%

• UNREALISTIC TO EXPECT ENZYMES TO ACHIEVE 100%

• REALISTIC TO EXPECT 25-35% OF UNDIG. FRACTION

THE BIRDpH Ret. Min %

CROP 5.7 10 to 50 17.5PROV 1.5

30 to 90 32GIZZ 2.7PDUO 4.6

5 to 10 3.5DDUO 6JEJEN 6.3 20 to 30 11ILEUM 6.7 50 to 70 25

CECA 8 20 to 30 11

THE ENZYMES

ENZYME TARGET SUBSTRATE TARGET FEEDSTUFF

PHYTASES PHYTIC ACID ALL PLANT INGREDIENTS

B-GLUCANASES B-GLUCAN BARLEY, OATS, RYE

XYLANASES ARABINOXYLANS WHEAT, BARLEY, RYE, TRITICALE, FIBROUS PLANT MATERIALS

PROTEASES PROTEINS ALL PROTEIN INGREDIENTS

AMYLASE STARCH CEREAL GRAINS, GRAIN LEGUMES

MANNANASES MANNANS SOYABEAN, PALM KERNAL

PHYTASE

Van Der Klis, J.D. and Star, L. (2012) Efficacy of different Phytase Products on ProductivityAnd Bone Ash in Broilers. Schothorst Feed Research Report No. 1186

PHYTASE

Van Der Klis, J.D. and Star, L. (2012) Efficacy of different Phytase Products on ProductivityAnd Bone Ash in Broilers. Schothorst Feed Research Report No. 1186

THE PHYTASE SUBSTRATE

PHYTASE

Walk, C.L., Bedford, M.R., Santos, T.S., Paiva, D., Bradley, J.R., Wladecki, H., Honaker, C. andMcElroy, A.P. (2013) Extra-phosphoric effects of superdoses of a novel microbial phytase.Poult. Sci. 92:719-725

PROTEASEPhase 2 FeedNutritional Level in the Feed AA Ratios RONOZYME ProAct Contribution Contribution of RONOZYME ProAct in FeedCP (%) 22 3.311 CP (%) 0.728 Lys Dig. (%) 1.290 2.823 Lys Dig. (%) 0.036 Met Dig. (%) 0.593 46% 3.749 Met Dig. (%) 0.022 M+C Dig. (%) 0.903 70% 5.424 M+C Dig. (%) 0.049 Thr Dig. (%) 0.826 64% 7.244 Thr Dig. (%) 0.060 Trp Dig. (%) 0.219 17% 1.680 Trp Dig. (%) 0.004 Arg Dig. (%) 1.445 112% 3.034 Arg Dig. (%) 0.044 Val Dig. (%) 0.993 77% 3.561 Val Dig. (%) 0.035 Leu Dig. (%) 1.213 94% 1.143 Leu Dig. (%) 0.014 Ile Dig. (%) 0.864 67% 2.921 Ile Dig. (%) 0.025 His Dig. (%) 0.477 37% 3.630 His Dig. (%) 0.017 G+S Dig. (%) 1.896 147% 3.836 G+S (%) 0.073

RONOZYME ProAct Dose RONOZYME ProAct Matrix Value 200 CP (%) 3,642

Lys Dig. (%) 182.084 Safety Margin to be consider (x*SD) Met Dig. (%) 111.237

0 M+C Dig. (%) 244.894 Thr Dig. (%) 299.037 Trp Dig. (%) 18.421 Arg Dig. (%) 219.201 Val Dig. (%) 176.834 Leu Dig. (%) 69.308 Ile Dig. (%) 126.227 His Dig. (%) 86.639 G+S (%) 363.684

THE PROTEASE SUBSTRATE

PROTEASE

PROTEASE

COMBINATION ENZYMES

• MULTIPLE COMPLEXES TARGETING THE SAME SUBSTRATE NOT BENEFICIAL

• XYLANASE + PHYTASE • IMPROVE PHOSPHORUS UTILIZATION THROUGH

INCREASING CELL WALL PERMEABILITY(Coppedge, J.R., Klein, J., Brown, B., Ratliff, B., Ruch, F. and Lee, J.T. (2011) Effects of co-administration of phytase and NSPase on broiler performance and bone ash. Int. J. Poult.Sci. 10:12:933-939)

• EXTENDED GASTRIC RESIDENCE TIME – PHYTASE POTENTIATING (WHEN NEEDED) AND IMPROVINGGASTRIC PROTEOLYSIS (WHEN NEEDED)(Kalmendal, R. and Tauson, R. (2012) Effects of a xylanase and protease, individually or in combination, and an ionophore coccidiostat on performance, nutrient utilization, and intestinal morphology in broiler chickens fed a wheat-soyabean-meal based diet. Poult. Sci. 91:1387-1393)(Mike Bedford, personal communication, 3/10/2013)

THE NSP SUBSTRATE

VARIABILITY IN RESPONSE

0

20

40

60

80

100

120

0,25 0,275 0,3 0,325 0,35 0,375 0,4 0,425 0,45 0,475 0,5Retainable Phosphorus %

OPTIMAL PHYTASE TEST SITUATION

Response

PCNC

CALCULATEDDEFICIENCY = 0.1%

ACTUALDEFICIENCY = 0.1%

VARIABLE + UN-PREDICTABLE

• ENZYME• SOURCE• DOSE RATE• SIDE ACTIVITIES

• DIET• INGREDIENT QUALITY• DIET COMPOSITION• FORM• PARTICLE SIZE• DENSITY

• BIRD• AGE• SEX• BREED

COMMERCIAL RESULTS

0,00

0,50

1,00

1,50

2,00

2,50

0 5 10 15 20 25 30 35 40

Live weight

Age

Rainbow KZN (Cycle 264-285)

Cobb Standard

B19 Trial 2011-014 (Cobb)

University Trial 2011-005 (Cobb)

COMMERCIAL TRIALS

COMMERCIAL TRIALS

COMMERCIAL TRIALS

COMMERCIAL TRIALS

METEN WETEN

ENZYME BENEFIT

• ENVIRONMENTAL IMPACT (N and P)

• COST REDUCTION / PERFORMANCE INCREASE

• REDUCTION OF WET LITTER COMPLAINTS

• INCREASED FEED INTAKE (PHYTASE)

• MORE CONSISTENT PERFORMANCE

• FORMULATION FLEXIBILITY

ENZYME BENEFIT

CONCLUDING REMARKS

• ACTIVE AREA OF RESEARCH AND DEVELOPMENT

• ROI

• ENZYME BENEFIT WELL RECOGNISED

• REALISTIC EXPECTATIONS

• STRATEGY IS TO INCREASE DIGESTION OFUNDIGESTED SUBSTRATES