8/6/2019 Galanthamine production by Leucojum aestivum in vitro shoot cultures

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Official Organ of theSociety for MedicinalPlant Researchwww.thieme-counect.com/ejournalswww.thiem e.dejfzjptanr.imedicaThis journal is indexedI Current Contents/lifeciences, Science CitationI rdex, MEDLINE, EMBASEand SCOPUS.

9 olume 74July 2008Page 895 --12287t oint Meeting ofAFER , SP, GA, PSE & SIFAugust 3-8, 2008, Athens, GreeceAbstractsCha irmen:Leandros Skalts un ISJoel ustieIssue ditors:Leandros SkaltsounisProkopios MagiatisIssue C -Edi or:Nikola Fol ialal i

PlanTa M.d ~ T h i e m e

8/6/2019 Galanthamine production by Leucojum aestivum in vitro shoot cultures

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RI 1 R=-OH( j , 11 2 R -COO: .--.---. /Y II (" 'I "H f)Ae/mowledgemencs: State Silo Paulo Research Foundation ( FAPESP). Brazilian National Counci l f or Research ( CNfJq). sao Paulo State Un;verslty ( Unesv ) and Universi ty or sao Pal/fo (USP). References: 1.Wanke, S. et al.(2007) Mo lecu lar Phyloge ne tics and Evolution 42 :477. 2. Lopes, A. et a l.Nat. Prod. Res, (s ubm it ted ), 3. Batista [uruor.]. et al. (2008) BioJ.Pharm.Bull. 31:538. 4. k uzuyama, T. er al. (2005) Nat ure 435 :")83. 5. M uhlenweg, A. et al, (1998 ) Planta 205:407.

G J, nthamine pr ur.t ion by Leu oj UFn (1 rivumin vitro ho t cu lturesGeorgiev V. Ivanov I. Pavlov A. ~ _ i e M, ttieva MDepartment Microb ia l Biosynthesis and Biote cllllologies tu bavotory in Plovdiv. Institute or Mic robiology, Balga rianAcademy of Sciences26 Marirza Blvd., 4002 Plovdiv,Bulga/ia

Calautham ine, an Amary liJdaceae alkaloid. become an impo rtant therapeutic optio ns used to slow down the process of neurological degenerat io n in Alzheimer's di sease. Although chemical syn thesis of galanrhamin e has been sucressful ly esrabhshcd, the main comm ercial source inBulgana for the produ cti on of gala nt ha rnine-bas ed medicines (undertrade name Nivaliu '") is Leucoj um aesti vum, an Euro-Medrrerranean species, also calle d sum mer snow flake. Since 1989, prescrip t ion regime o fthe ut ilization of this plant species has been imposed and, from theseperspectives, ga lan t ham me production from in vitro cultures is considered as an alte rna tive. In vitro call us and shoot cultu res of L. aest vulllpreviously obta ined w ere checked for galanthamine production. It wasestablished that the amoun t of accumulated galantham ine strongly depended on the level of differentiation. The maximum amou nt of accumul ared biomass (15.6 gj Ll. the endo-galanrhami ne content (55.6 pg/g)and the exo-galanth arnine yie ld (1.6 mg/L) were achieved afte r 5 weeksof subm erged cul ti vat ion o f 1.. aestivum 80 shoot cul tu re, in a m odifiedMS medium. und er illumination.

Further the galanthami ne bloproducrion process w up-scaled in di fferent cul tivat ion systems, such as column bioreactor and RITA''' temporary Inlm erSlon systems. Based on the obta ined re.sults, the in vitroshoot cultures of L aestiv llln could be considered as an attract ive altel nati ve for galantham ine producti on. Ackn OWledgements: Tllis researdlhas been support ed by a Ma rie Cude Eur opean Re-Integmtion GranL undercontraU num ber MERC-CT-200? - 046427.

Pseu doJujubogelllti gly os i es tro m in vUror general I Bacopa monn eri ILl Wettsl![jo r i pension cul[ures of C. wigll tii, grown in modified MS ~ i u

Planta Med 2008: 74: 895- 12.2.7 Georg Thieme Verlag KG 5tllttgMt . New Yorl< . 155N 0032-0943