Golgi apparatusStructure

– Consist of flattened cavities called cisternae lined with smooth endoplasmic reticulum

– Numerous vesicles containing secretory granules are pinched off at the edges

Formation– Formed by the fusion of vesicles pinched off

from the cavities of ER

Source: Cells Alive

Functions• Site for concentrating and packing the

materials in other parts of the cell e.g. proteins newly synthesized in the rough ER are transported in the vesicles to the Golgi apparatus where carbohydrates are added to form glycoproteins which are then packed in vesicles for discharge -May play a part in primary wall formation -Lipid transport -Lysosome formation

The Golgi Apparatus

• Because of its large and regular structure, the Golgi apparatus was one of the first organelles described by early light microscopists.• It consists of a collection of flattened, membrane-enclosed cisternae, somewhat resembling a stack of pancakes. Each of these Golgi stacks usually consists of four to six cisternae• Each Golgi stack has two distinct faces: a cis face (or entry face) and a trans face (or exit face). Both cis and trans faces are closely associated with special compartments, each composed of a network of interconnected tubular and cisternal structures.

Transport of proteins from ER to Golgi

• Proteins destined for the Golgi, lysosome, PM, or extracellular fluid are packaged into vesicles at specialized sites referred to as ER EXIT SITES.• ER exit sites are studded with receptors which bind to proteins destined to leave the ER. Proteins leaving the ER contain specific amino acid sequences which are bound by these receptors.• Binding the receptor induces vesicle budding and the transport of the vesicle to the cis-Golgi network. It is important to note that only properly folded proteins are transported.• Following vesicle budding, vesicles fuse to form a vesicular tubular cluster which is then transferred to the Golgi.

Golgi Apparatus

• The Golgi apparatus receives newly made proteins and lipids from the ER, puts the finishing touches on them, addresses them, and sends them to their final destinations.

Golgi Apparatus

• The Golgi apparatus processes and packages proteins.

Vesicles

• Vesicles, including lysosomes (digestive enzymes) and peroxisomes (detoxification enzymes), are classified by their contents.

Vesicles, continued

Protein Synthesis– The rough ER, Golgi apparatus, and vesicles work together

to transport proteins to their destinations inside and outside the cell.

Chapter 4

Processing of Proteins

Golgi Apparatus

• A Golgi complex is located beneath a centriole.

• Many of the Golgi sacs are distended, probably because they were filled with protein for secretion.

Golgi/Endoplasmic Reticulum

• A Golgi complex in the center of the field is surrounded by RER and SER.

• The black dots are NOT glycogen, but packaged protein. (They are membrane enclosed.)

Golgi Apparatus

• This Golgi complex shows very distended saccules.

• Note the extensive RER surrounding it.

Golgi Apparatus• Stacked and flattened

membranous sacs with cisternae• Functions in modification,

packaging, distribution of proteins and lipids for secretion or internal use

• Transport vesicles from ER fuse with Golgi body

• After modification protein is packaged in secretory vesicle (a budding of the Golgi) and move to designated parts of the cell

• Some secretory vesicles may accumulate in cell waiting for signal to release their contents (e.g.,blood sugar levels and insulin release)

Role of the Golgi Apparatus

Figure 3.21