foodsniffer food safety at the point-of-need via monolithic spectroscopic chip identifying harmful...

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FOODSNIFFE R FOOD Safety at the point-of-Need via monolithic spectroscopic chip identiFying harmFul substances in frEsh pRoduce NCSR ‘Demokritos’, Greece Coordination – Optoelectronic Sensor Chip Design & Fabrication -Immnoassay Protocols & Surface Functionalization VTT Technical Research Center of Finland Chip Design & Fabrication – Reader Electronics TRUSTFOOD, The Netherlands Smartphone Application – Dissemination & Exploitation Activities Jobst Technologies GmbH, Germany Microfluidics & Filtration Modules LioniX BV, The Netherlands Optoelectronic Chip Evaluation RIKILT, The Netherlands Allergens and Mycotoxins Expert – Sample Extraction Protocols EUROFINS, France System Evaluation & Correlation to Established Methods University of Almeria, Spain Sample Extraction – Validation Methods – Quality Control Jagiellonian University, Poland Sensor surface characterization ThetaMetrisis SA, Greece System Integration & Reader Development Concern for our food is growing in Europe, driven by industrialized food production and repeated crises. Current analytical technology is too expensive and bound to laboratory tests. This problem requires more massive screening of food and water extending from the source to the point of consumption. A low-cost and portable system delivering analytical data to a central location would help to prevent or identify early any food safety threat outbreaks and thereby massively reduce human suffering and its associated financial cost on both sides of the global divide. FOODSNIFFER is field-deployable and simple-to-use as a result of the integration of three major innovations: the all-silicon optoelectronic transducer based on Broad-Band Mach-Zehnder Interferometry that integrates the light-sources, sensing elements, spectral analyzer and photodetectors, in a single chip and is capable of synchronous highly-sensitive label-free multi- analyte detection the wafer-scale microfluidics and filtration systems into a disposable unit that unburden intensive sample preparation the development of a low-power reader controlled by a smartphone through a custom-produced application. The software controls the sensor and also processes its signal and then sends the results securely via the internet during the on-the-spot food safety analysis. FOODSNIFFER is a complete business solution which will be demonstrated in three areas of great importance to European society and regulators, viz. the detection of pesticide residues, mycotoxins and allergens in selected food categories in order to demonstrate field-based detection of harmful species at low concentrations, which is a feat unattained so far by any point-of-need system. For further information please contact: Ioannis Raptis INN, NCSR ‘Demokritos’ End Patriarchou Gregoriou Str. 153 43 Aghia Paraskevi, Greece E-mail: [email protected] Tel: +30-210-6503265 Detection of allergens in food: detection of goat milk adulteration with bovine milk Detection of mycotoxins in beers and cereals: ochratoxin A detection in beer Detection of pesticide residues in grapes and wines: thiabendazole detection in wine Detection limit achieved: 0.04% (v/v) bovine milk in goat milk. Maximum allowable concentration of bovine milk to goat milk for adulteration control <1% (v/v) Detection limit achieved: 10 ng/mL OTA in beer Detection limit achieved: 1.5 ng/mL in wine (30- times dilution with assay buffer) Maximum allowable concentration for grapes and wines: 50 ng/g 0 25 50 75 100 0.0 0.2 0.4 0.6 0.8 1.0 0.2% 0.5% 0.1% 0% Tim e (sec x 10) R adians 0.0 0.1 0.2 0.3 0.4 0.5 10 20 30 40 50 60 70 80 90 100 (S x /S 0 )% cow m ilk % 0 40 80 120 160 200 0.0 0.2 0.4 0.6 0.8 1.0 1.2 R adiands Tim e (sec x 10) assay buffer 1stAb 2nd A b 0.0 0.2 0.4 0.6 0.8 1.0 10 20 30 40 50 60 70 80 90 100 (S x /S 0 )% T h ia b e n d a zo le co n ce n tra tio n (n g /m L) 0 20 40 60 80 100 10 20 30 40 50 60 70 80 90 100 (S x /S 0 ) % O TA in beer (ng/m l) 0 100 200 300 -0 .2 -0 .1 0.0 0.1 0.2 0.3 0.4 0.5 OTA 20 n g /m L M ab + beer sam ple T im e (x10 s) R adians b ee r diluted 1 6-tim e s in a ssa y b u ffe r a ssa y b u ffe r an ti-m o u se Ig G Ab OTA 0 ng /mL

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Page 1: FOODSNIFFER FOOD Safety at the point-of-Need via monolithic spectroscopic chip identiFying harmFul substances in frEsh pRoduce NCSR ‘Demokritos’, Greece

FOODSNIFFERFOOD Safety at the point-of-Need via monolithic spectroscopic

chip identiFying harmFul substances in frEsh pRoduce

NCSR ‘Demokritos’, GreeceCoordination – Optoelectronic Sensor Chip Design & Fabrication -Immnoassay Protocols & Surface Functionalization

VTT Technical Research Center of Finland Chip Design & Fabrication –Reader Electronics

TRUSTFOOD, The NetherlandsSmartphone Application – Dissemination & Exploitation Activities

Jobst Technologies GmbH, GermanyMicrofluidics & Filtration Modules

LioniX BV, The NetherlandsOptoelectronic Chip Evaluation

RIKILT, The NetherlandsAllergens and Mycotoxins Expert – Sample Extraction Protocols

EUROFINS, FranceSystem Evaluation & Correlation to Established Methods

University of Almeria, SpainSample Extraction – Validation Methods – Quality Control

Jagiellonian University, PolandSensor surface characterization

ThetaMetrisis SA, GreeceSystem Integration & Reader Development

Concern for our food is growing in Europe, driven by industrialized food production and repeated crises. Current analytical technology is too expensive and bound to laboratory tests. This problem requires more massive screening of food and water extending from the source to the point of consumption. A low-cost and portable system delivering analytical data to a central location would help to prevent or identify early any food safety threat outbreaks and thereby massively reduce human suffering and its associated financial cost on both sides of the global divide.

FOODSNIFFER is field-deployable and simple-to-use as a result of the integration of three major innovations: the all-silicon optoelectronic transducer based on Broad-Band Mach-Zehnder Interferometry that integrates the light-sources,

sensing elements, spectral analyzer and photodetectors, in a single chip and is capable of synchronous highly-sensitive label-free multi-analyte detection

the wafer-scale microfluidics and filtration systems into a disposable unit that unburden intensive sample preparation

the development of a low-power reader controlled by a smartphone through a custom-produced application. The software controls the sensor and also processes its signal and then sends the results securely via the internet during the on-the-spot food safety analysis.

FOODSNIFFER is a complete business solution which will be demonstrated in three areas of great importance to European society and regulators, viz. the detection of pesticide residues, mycotoxins and allergens in selected food categories in order to demonstrate field-based detection of harmful species at low concentrations, which is a feat unattained so far by any point-of-need system.

For further information please contact:Ioannis RaptisINN, NCSR ‘Demokritos’End Patriarchou Gregoriou Str.153 43 Aghia Paraskevi, GreeceE-mail: [email protected]: +30-210-6503265

0 25 50 75 100

0.0

0.2

0.4

0.6

0.8

1.0

0.2%

0.5%

0.1%

0%

Time (sec x 10)

Rad

ians

Detection of allergens in food: detection of goat milk adulteration with bovine milk

0.0 0.1 0.2 0.3 0.4 0.510

20

30

40

50

60

70

80

90

100

(Sx/S

0)%

cow milk %

Detection of mycotoxins in beers and cereals: ochratoxin A detection in beer

Detection of pesticide residues in grapes and wines: thiabendazole detection in wine

0 40 80 120 160 200

0.0

0.2

0.4

0.6

0.8

1.0

1.2

Rad

iand

s

Time (sec x 10)

assaybuffer

1st Ab

2nd Ab

0.0 0.2 0.4 0.6 0.8 1.010

20

30

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50

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(Sx/S

0)%

Thiabendazole concentration (ng/mL)

0 20 40 60 80 10010

20

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80

90

100

(S

x/S

0)

%

OTA in beer (ng/ml)

Detection limit achieved: 0.04% (v/v) bovine milk in goat milk.

Maximum allowable concentration of bovine milk to goat milk for

adulteration control <1% (v/v)

Detection limit achieved: 10 ng/mL OTA in beer

0 100 200 300-0.2

-0.1

0.0

0.1

0.2

0.3

0.4

0.5

OTA 20 ng/mL

Mab

+ beer sample

Time (x10 s)

Rad

ians

beer diluted

16-times in

assay bufferassay buffer

anti-mouse IgG Ab

OTA 0 ng/mL

Detection limit achieved: 1.5 ng/mL in wine (30-times dilution with assay

buffer)Maximum allowable concentration

for grapes and wines: 50 ng/g