figure s1_yao qin et al. figure s1 occurrence and distribution of trihelix family in different plant...
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Figure S1_Yao Qin et al.
Figure S1 Occurrence and distribution of trihelix family in different plant species. Red branches in the cladogram indicate that no trihelix members were found, while blue branches indicate presence of trihelix members. Green dots mark important events in plant evolution. The table shows in which species each clade of trihelix members can be found.
Figure S2_Yao Qin et al.
α-1 α-2 α-3
Clade IN
……
……
……
……
*
Clade IC
Clade II
Clade V
Clade III
Clade IV
* *
* * * *
* ** *
* * *
* ** *
* ** *
……
*
20 a.a
Figure S2 Sequence logo of the DNA binding domain of each clade. N, DNA binding domain at N terminal of clade I. C, DNA binding domain at C terminal of clade I. The trihelix domains were underlined by red. The fourth amphipathic a-helix, with the general sequence (F/Y)-(F/Y)-X-X-(L/I/M)-X-X-(L/I/M), is shown in green. The predicted coiled-coils motifs were underlined by blue.
Figure S3 Genomic organization and colinearity of the trihelix family in rice, brachypodium and sorghum. Chromosomal localization of trihelix genes was indicated in each species. Green color represents for orthologous pairs between rice and sorghum, orange for rice and brachypodium, blue for brachypodium and sorghum.
Figure S3_Yao Qin et al.
Clade I
Clade II
Clade V
Clade III
Clade IV
BA C
Figure S4_Yao Qin et al.
Figure S4 Structural Analysis of Trihelix proteins in each clade Via phylogenetic analysis. (A) The constructed 3D structure of Trihelix by homology modeling by 2D alignment to template protein 2JMW. (B) Ramachandran plots of the Trihelix model. The different colored areas indicate “most favored allowed” (blue)” , and “additional allowed” (purple red) regions and the disallowed regions were marked in red plots. (C) Profile scores of the Trihelix mode. Each amino acid of Trihelix protein model was scored and the score above 0.2 was assumed to be confidential.
Figure S5_Yao Qin et al.
Figure S5 Heat map of tissue specific expression profile of Arabidopsis trihelix factors using microarray data. Expression was presented by heat maps in blue/red colors generated using the meta-analysis tool at Genevestigator http://www.genevestigator.ethz.ch. The color intensity corresponds to the expression level.
9741
48
21
5917
12
156
2579
76
24
6704
11
57
5733
23
71
2519
94
2 47
18
4311
21
869
6883
66
38
4917
22
70
2165
15
430
8722
22
84
6952
77
28
I II IVIII V
AT
1G76
890
AT
1G76
880
AT
5G28
300
AT
1G33
240
AT
5G03
680
AT
5G63
420
AT
1G13
450
AT
3G25
990
AT
2G38
250
AT
5G01
380
AT
1G21
200
AT
1G76
870
AT
3G10
040
AT
4G31
270
AT
1G31
310
AT
1G54
060
AT
3G14
180
AT
3G11
100
AT
5G05
550
AT
3G58
630
AT
3G24
490
AT
2G44
730
AT
3G54
390
AT
3G10
030
AT
4G17
060
Figure S6_Yao Qin et al.
Figure S6 Heat map of stimulated expression profile of Arabidopsis trihelix factors using microarray data. Expression was presented by heat maps in blue/red colors generated using the meta-analysis tool at Genevestigator http://www.genevestigator.ethz.ch. The color intensity corresponds to the expression level.
9741
4821
5917
1215
6
2579
7624
6704
1157
5733
4718
2519
942
2186
9
6883
6638
4917
2270
2165
1543
0
8722
2284
6952
7728
I II IVIII V
AT
1G76
890
AT
1G76
880
AT
5G28
300
AT
1G33
240
AT
5G03
680
AT
5G63
420
AT
1G13
450
AT
3G25
990
AT
2G38
250
AT
5G01
380
AT
1G21
200
AT
1G76
870
AT
3G10
040
AT
4G31
270
AT
1G31
310
AT
1G54
060
AT
3G14
180
AT
3G11
100
AT
5G05
550
AT
3G58
630
AT
3G24
490
AT
2G44
730
AT
3G54
390
AT
3G10
030
AT
4G17
060
Figure S7_Yao Qin et al.
Figure S7 Heat map of tissue specific expression profile of rice trihelix factors using microarray data. Expression was presented by heat maps in blue/red colors generated using the meta-analysis tool at Genevestigator http://www.genevestigator.ethz.ch. The color intensity corresponds to the expression level.
3388
460
261
3764
141
462
3891
874
897
1667
826
888
8895
3732
637
772
1630
10
790
4291
528
1720
23
958
6838
165
173
3616
60
644
1118
713
3707
4578
2 33
727
7458
30
0038
30
12
I II IVIII V
Os0
4g45
750
Os0
2g01
380
Os0
2g43
300
Os0
3g02
240
Os1
0g37
240
Os0
2g33
610
Os0
4g40
930
Os0
4g51
320
Os1
2g06
640
Os1
1g06
410
Os0
2g33
770
Os0
1g21
590
Os0
4g32
590
Os0
2g31
160
Os0
1g70
230
Os0
3g46
350
Os0
2g07
800
Os0
5g48
690
Os0
1g48
320
Os0
4g36
790
Os0
2g35
690
Os0
1g52
090
Os1
0g41
460
Os0
8g37
810
Os0
9g38
570
Os0
4g45
940
Os0
4g45
940
Os0
3g18
340
Figure S8_Yao Qin et al.
Figure S8 Heat map of stimulated expression profile of rice trihelix factors using microarray data. Expression was presented by heat maps in blue/red colors generated using the meta-analysis tool at Genevestigator http://www.genevestigator.ethz.ch. The color intensity corresponds to the expression level.
3388
4 60
261
3764
1 41
462
3891
8 74
897
1667
8 26
888
8895
37
326
3777
2 16
30
1079
0 42
91
528
1720
23
958
6838
1 65
173
3616
60
644
1118
7 13
3707
45
782
3372
7 74
58
3000
38
3012
I II IVIII V
Os0
4g45
750
Os0
2g01
380
Os0
2g43
300
Os0
3g02
240
Os1
0g37
240
Os0
2g33
610
Os0
4g40
930
Os0
4g51
320
Os1
2g06
640
Os1
1g06
410
Os0
2g33
770
Os0
1g21
590
Os0
4g32
590
Os0
2g31
160
Os0
1g70
230
Os0
3g46
350
Os0
2g07
800
Os0
5g48
690
Os0
1g48
320
Os0
4g36
790
Os0
2g35
690
Os0
1g52
090
Os1
0g41
460
Os0
8g37
810
Os0
9g38
570
Os0
4g45
940
Os0
4g45
940
Os0
3g18
340