Figure S1 Approximate positions of primers listed in Supplementary Table S1 for F3‘H (a), Tdic101 (b) and dTdic102 (c). Black arrow heads show primers and their orientation. Coding regions (shaded boxes) in F3'H, the transposase (black bar) in Tdic101 and the TIRs (terminal inverted repeats, open triangles) are indicated.

a

b c

9

15 1916 18

137

4

1

2

(G)HindIII HindIII

3 56

8

10

14

Tdic101 insertion site

1720

21

23

HindIII

11

122224

2526

27 28

29

30

3132

33

F3‘H

Tdic101 dTdic102

0.81.1

23.1

6.6

2.3

9.4

2.0

(kbp)

MPu MPi 1 2 3 4 5 6 7 8

Fig. S2 Analysis of the vacated site of the 4.3 kb (Tdic101) insertion in the F3‘H gene. PCR products are shown that are obtained with gene-specific primers on DNAs isolated from six different reverted, purple regions emerging in MPi flowers (1-6) or from pink regions in partly reverted variegated MPi flowers (7-8). Analysis by PCR of DNA isolated from MPu and MPi is shown and the migration of molecular weight markers is indicated on the left.

Fig. S3 Schematic structure of the putative Tdic101 transposase (TPase). Black boxes represent the six conserved hAT-transposase motifs reported by Rubin et al. (2001). The number of amino acids (aa) separating these motifs in the Tdic101 TPase are indicated.

A; CKFNLTADVWDS D; VLDLLLWWKGQB; HVRCACHVLNLCV E; LPVMSAMAQDFLSIQVSSVASERAFSC; TRWNSMYIML F; SKRVLDEKRTSLRSDTLEMCVCYKDW

A B C D E F

88 aa 50 aa 226 aa 3 aa 1 aa320 aa 28 aa

TPase (814 aa)