figure legend fig. s1

Heterologous expression system in Aspergillus oryzae for fungal biosynthetic gene clusters of secondary metabolites Applied Microbiological Biotechnology Kanae Sakai, 1 Hiroshi Kinoshita, 1 and Takuya Nihira 1,2* 1 International Center for Biotechnology, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan 2 MU-OU Collaborative Research Center for Bioscience and Biotechnology, Faculty of Science, Mahidol University, Rama VI Rd., 10400 Bangkok, Thailand *Corresponding author. Tel.: +81-6-6879-7452; Fax: +81-6-6879-7454; E-mail: [email protected]

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Heterologous expression system in Aspergillus oryzae for fungal biosynthetic gene clusters of secondary metabolites

Applied Microbiological Biotechnology Kanae Sakai,1 Hiroshi Kinoshita,1 and Takuya Nihira1,2*

1International Center for Biotechnology, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan2MU-OU Collaborative Research Center for Bioscience and Biotechnology, Faculty of Science, Mahidol University, Rama VI Rd., 10400 Bangkok, Thailand

*Corresponding author. Tel.: +81-6-6879-7452; Fax: +81-6-6879-7454; E-mail: [email protected]

Figure Legend

Fig. S1.

Representative genotype of transformants. (a) Southern blot analysis of the MK transformants. Southern blot analysis was carried

out against MluI-digested genomic DNA using the mokB fragment as the probe. The mokB fragment was amplified by PCR with

primers mokB-f and mokB-r. Lanes C1, cosmid 12-33; C2, cosmid sCnDmokB; W, A. oryzae NS4 strain; M, λ-EcoT14I digest DNA

marker; 1, M12-33-5; 2 to 4, candidate transformants (MK5-1,3,4); 5, M12-33-17; 6 to 9, candidate transformants (MK17-1, 2, 5,

6); 10, M12-33-18; 11, candidate transformants (MK18-1). The bands indicated by arrow were derived from cosmid 12-33. The

band indicated by arrowhead was derived from sCnDmokB. (b) Southern blot analysis of the MK-L transformants. Southern blot

analysis was carried out against SnaBI-digested genomic DNA using the AolaeA PCR fragment (amplified by AolaeA-3f: 5’-

atggcctaatgtacgccc-3’, AolaeA-3r: 5’-ctcctgcagagtctcggat-3’) as the probe. Lanes V, pPTRI-laeA; M, λ-EcoT14I digest DNA

marker; 1, M12-33-17 strain; 2, MK-L1; 3, MK-L2.

The band indicated by arrow was derived from pPTRI-laeA. The band indicated by arrowhead was the signal of native laeA gene.

(c) Southern blot analysis of KsMK transformants. Southern blot analysis was carried out against EcoRI-digested genomic DNA

using the mokA or mokB2 PCR fragment (amplified by mokA-f, -r or mokB-2f, -2r primer sets) as the probe. Lanes: H, A. oryzae

host strain (Ks31); 6, 21, candidate transformants (KsMK-6, 21). (d) Southern blot analysis of TQ transformants. Southern blot

analysis was carried out against KpnI-digested genomic DNA using the tdiD PCR fragment as a probe. Lanes: V, TQ gene cluster

expression vector sCsCTQ; M, λ-EcoT14 digest DNA marker; H, Ks31 host; 25, 33, 34,36, 39, 47, 52, 53, candidate transformants

(KsTQ-25, 33, 34, 36, 39, 47, 52, 53). The arrowhead indicated the position of 9.4 kb.

FIG. S2. 1H-NMR spectrum of MK purified A. oryzae transformant KsMK-25

FIG. S3. 1H-NMR spectrum of MJ purified A. oryzae transformant KsMK-25

FIG. S4. 1H-NMR spectrum of TQ purified A. oryzae transformant KsTQ-39

FIG. S1

mokA

mokB2

(probe)

c6H 21

b MV 1 2 3

C1 C2 WM 1 2 3 4 5 6 7 8 9 10 11

V 25 33 34 36 39 47 52 53H

FIG. S2

FIG. S3

FIG. S4

Dierks Supplementary Fig. S1 - Cancer Research...Dierks Supplementary Fig. S1 ITK SYK PH K402R E42K R29C TH wt K402R (kinase deficient) E42K R29C (reduced phospholipid binding) E42K

Cambridge University Presscambridge... · Web viewS1. Melting curves and standard curves of reference and target genes. Fig. S1 A. Melting curve & standard curve of ALP Fig. S1 B

Supplemental Figures S1-S6aem.asm.org/content/suppl/2011/07/20/77.15.5207.DC... · Supplemental Figure Legend S1. Vero cells infected by Rickettsia monteiroi as demonstrated by Gimenez

Fig. S1. Co-purification of 6His-Scy with ParA and …rsob.royalsocietypublishing.org/content/suppl/2013/03/26/...Fig. S1. Co-purification of 6His-Scy with ParA and ParA Scy-from S

Supplementary Fig. S1

Tholen et al ., Sup . Fig. S1

S1 Fig. Hydrolysis of α2-3-linked sialic by high amounts ...nizetlab.ucsd.edu/Publications/Neu5GC-Cholera-Suppl.pdf · S1 Fig. Hydrolysis of α2-3-linked sialic by high amounts of

Figure S1 Enod11 Mtc27 MtGshs cDNA gDNA S. meliloti DPI --++ - + + - Supporting Information Fig. S1. Validation of the selected biological conditions for

Supplemental Figure Legend Figure Legend Fig. S1. Control gel electrophoretic analysis of free Ph-P0 samples. The samples (each including 100 pmol of Ph-P0 or its mutants) of Ph-P0.Ph-L12

Fig. S1 . Okubo et al

Supplementary Fig. S1

Suppl. Fig. S1 Suppl. Fig. S1 The nucleotide sequence and its deduced amino acid sequences of CaSAMDC. The full-length of CaSAMDC (GenBank Accession No

Fig S1 Figure S1: 5T4 expression in B cell development. Adapted from microarray data published online by van Zelm et al

PRISMA 2009 Flow Diagramcambridge.org:… · Web viewAnxiety disorders. Supplementary Fig. S1. PRISMA 2009 Flow Diagram Supplementary Table S1. Anxiety disorder studies. Authors

Pandolfini et al., Control by RISC stabilizes pluripotency10.1186/s13059-016-0952... · Pandolfini et al., Control by RISC stabilizes pluripotency Fig. S1 - Legend A, Western blot

Supplemental Figure Legends Fig. S1 · Supplemental Figure Legends . Fig. S1. A: Representative polyacrylamide gel showing cleavage of 5 µM DPRSFL-ACPP by several enzymes known to

Fig. S1. Effect of TG2 expression on LNCaP cell growth (A) and

Fig S1:Determination of population sub-structure

Fig. S1. Effects of loss of core planar polarity activity ...dev.biologists.org/highwire/filestream/1203774/field_highwire... · Fig. S1. Effects of loss of core planar polarity activity

Supplemental Fig. S1

Fig. S1. Typical total ion chromatograms (TIC) obtained

FIG S1 Protein sequence alignment of AgaO and characterized … · 2016-07-20 · FIG S1 Protein sequence alignment of AgaO and characterized GH50 β-agarases. Amino acid residues

Supporting Information Figs S1–S8, Tables S1–S4 2 3 Fig. S1 · 1 1 Supporting Information Figs S1–S8, Tables S1–S4 2 3 Fig. S1 4 5 Fig. S1 Phenotypes of wild type and wp1

Fig S1 120414 - CSHL P

Supporting Information - Royal Society of Chemistry2 / 9 Figure Legends and Video Captios Fig. S1 1H NMR spectra of NLC. Fig. S2 1H NMR spectra of NDC. Table S1 Gelation behaviors,

Control #A A5 2 wk p.i. Brain Spleen A2 12 wk p.i. Sakuma et al., Supplementary Fig. S1 Fig. S1 Immunostaining of brain and spleen sections with goat anti-gp70/gp30

Fig. S1 TGA curves (DTA and weight loss) for blends of PVC

Supplementary Fig S1 . Phase Contrast Photograph of Bacillus lonarensis sp. nov . 25nlg T

Fig. S1 Overview of small RNA sequencing data

Fig. S1: Hourly temperature (˚C) and broadband sound

Supplemental Methods Fig. S1. WGCNA gene dendrogram

Supplementary Fig 1 (S1) · Supplementary Fig 1 (S1): Allele-specific score prediction for endometriosis, using Stage A endometriosis cases in the OX case-control set as the target

Supplementary figures: Fig. S1. Human genes highly ......Expression of 582 HPV-correlated genes in JHU OPSCC and TCGA CESCC cohorts. Fig. S1. Human genes highly correlated with HPV

Fig. S1 Fig. S1 Correlation between microarray and qRT-PCR expression analyses. The linear regression compares expression ratios of salinity to control

SUPPLEMENTAL DATA - FIG S1-3dm5migu4zj3pb.cloudfront.net/manuscripts/63000/63924/JCI63924sd.pdf · SUPPLEMENTAL DATA Figure S1. Characterization of Vhl-/-mice. (A) Left panel shows