hnolo

fructose) (Fonseca, 2002). Procedure for starch extraction used byTyler, V.E. and Schuaring (Tyler and Schuaring, 1962); Understand-ing Ethanol: Fuel Production and Use, ISBN: 0-86619-203-4 By CliffBradley & Ken Runnion (Anon, in press-a); Production systems usedto obtain ethanol By MAIZAR (Maize Argentinean Association – Aso-ciación de Maíz Argentino) (Anon, in press-b); Procedure for starchextraction used by Linde et al. (in press).

Abstracts / Journal of Biotec

ran and isopropyl alcohol (IPA), IPA was found to be best solvent forpullulan precipitation. Different ratios of fermented broth to IPAwere tested for pullulan precipitation and ratio 1:2 was found bestafter 12 h of precipitation time. Characterization of the exopolysac-charide was carried using IR Spectroscopy. IR spectra of EPS fromisolate was similar to that of the pullulan standard (Sigma, USA),with a pullulan-like peak at around � = 850 cm−1 indicating the�-configuration within the exopolysaccharide.

Reference

Singh, R.S., Saini, G.K., Kennedy, J.F., 2008. Pullulan: microbial sources, productionand applications. Carb. Poly. 73 (4), 515–531.

doi:10.1016/j.jbiotec.2008.07.625

V7-O-016

Precise monitoring system for biogas engineering studies: Pro-duction enhancement by ultrasound treatment

Ales Prell 1,∗, Pavel Ditl 1, Petr Kujan 1, Miroslav Sobotka 1, MartinZavacky 2

1 Institute of Microbiology ASCR, v.v.i., Videnska 1083, CZ-14220Prague, Czech Republic2 Czech Technical University – Faculty of Mechanical Engineering,Technicka 4, CZ-16607 Prague, Czech Republic

E-mail address: prell@biomed.cas.cz (A. Prell).

Alternative energy productions are coming to relevance slowly butsurely in the changing world. At the same time, efficient remov-ing of various biological waste is still a current issue of the heavilypopulated Europe and dramatically evolving world parts. Tech-nologies of biogas production are known and used for years butjust the unstable marked energy equilibrium decides of sink orswim of these beneficial biotechnological applications. Thereforea detailed knowledge of the process efficiency and any economi-cal improvement are welcomed (Thran and Kaltschmitt, 2007). ThePilot-Plant Department of the Institute of Microbiology in Pragueis oriented to and equipped mainly to aerobic microbial technolo-gies but there is no impediment to adapt aerobic bioreactors toanaerobic digestive ones employing all monitoring and automation

elements and supplying new ones, needed for a precise quantitativeanalysis of the anaerobic processes. CSTR bioreactors Bioengineer-ing 75 L (Bioengineering AG, Wald, Switzerland) were equipped byadvanced automation including monitoring and control of basicfermentation parameters (Prell et al., 2006). In this step, we sup-plied sensors for humidity, temperature and pressure of leaving gasdirectly upon the liquid level and the same set behind a back-coolerand back-off regulatory valve, then a controlled biogas composi-tion analyzer follows. To control these new measuring elementswe needed to implement a new process computer Tecomat TC700(Teco Ltd., Kolin, Czech Republic), to develop a specific operatingsoftware and integrate it to our control system of the bioreactorsand the supervisory SCADA system. The original measuring andautomation equipment was developed to provide precise quantita-tive studies of biogas production. This allows to optimize anaerobicbioprocesses to be able to compete with other alternative energysources. The whole approach would be scalable applicable underreal conditions. Comparative experimental runs with model andreal media were performed to prove a supposed influence of ultra-sound treatment of fermentation broth under optimized intensityto improve and accelerate biogas production.

gy 136S (2008) S506–S518 S507

References

Prell, A., Sobotka, M., Kujan, P., Safar H., Holler, P., 2006. New built bioreactor controlsystems for Bioengineering equipment. Proc. 7th PROCESS CONTROL ‘06, Koutynad Desnou, Czech Republic, Lecture R027.

Thran, D., Kaltschmitt, M., 2007. Competition—supporting or preventing anincreased use of bioenergy? Biotechnol. J. 2, 1514–1524.

doi:10.1016/j.jbiotec.2008.07.626

V7-Y-090

Ethanol production from hydrolysis, fermentation and distilla-tion by reduced pressure of the potato starch

Martinez Viviana

National University of Colombia, Colombia

E-mail address: vmmartinezga@unal.edu.co.

The proposal of this work is the improvement of the method forethanol production from renewable resources, like potato starch.This as response to the consequences that entails using fosile fuels.There are suggested modifications of the methods described ByBradley (Anon, in press-b), increasing the efficiency in the ethanolproduction. Since, the problem is the cost of the process, because“Actually, around 40 and 48% of glucose becomes ethanol, with a45% of fermentation effectiveness” (Anon, in press-b), the hypoth-esis is that when working the divided distillation described inprocess (Anon, in press-b), as a distillation by reduced pressure,and replacing the material of the separation column by borosili-cate; will be obtained a greater yield in the azeotropic distillation,due to the considerable pressure diminution, temperature increaseand resistance of materials increase.

The percentage of starch in the sample (10.64%) was smaller thanexpected aprox. 60%, but to compare these percentages should betaken into account certain factors such as storage time as duringstorage are acting enzymes that allow degradation starch polysac-charides to smaller (sucrose) and monosaccharides (glucose and

References

Anon. http://sleekfreak.ath.cx:81/3wdev/VITAHTML/SUBLEV/ES1/ETHANOL.HTM.Anon. http://www.maizar.org.ar/vertext.php?id=246.Fonseca, A.M., Urena Avila, A., Determination of change in the content of starch and

sugars during storage R-12 from four geographical areas Colombian, NationalUniversity of Colombia, faculty of chemistry, Bogotá D.C., 2002, pp. 45–49.

Linde, M., Galbe. M., Zacchi, G., Bioethanol production from non-starch carbohydrateresidues in process streams from a dry-mill ethanol plant. Elsevier Journal ofBiotechnology, Methods.

Tyler, V.E., Schuaring, A.E., 1962. Experimental Pharmacognosy. Burges PublishingCompany.

doi:10.1016/j.jbiotec.2008.07.627