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    BY: ALOK SAXENA

    http://www.google.com/imgres?imgurl=http://obit-mag.com/media/image/Lenins-Body-Moscow-420x0.jpg&imgrefurl=http://obit-mag.com/articles/galileos-finger-confessions-of-a-necro-tourist&usg=__sGJviV1m1mgWz_cgSwLwBuW0-2c=&h=279&w=420&sz=10&hl=en&start=1&um=1&itbs=1&tbnid=xFhT4l7IV0KfMM:&tbnh=83&tbnw=125&prev=/images%3Fq%3Dpictures%2Bof%2Blenin%2Bbody%26um%3D1%26hl%3Den%26biw%3D1276%26bih%3D540%26tbs%3Disch:1http://www.google.com/imgres?imgurl=http://obit-mag.com/media/image/Lenins-Body-Moscow-420x0.jpg&imgrefurl=http://obit-mag.com/articles/galileos-finger-confessions-of-a-necro-tourist&usg=__sGJviV1m1mgWz_cgSwLwBuW0-2c=&h=279&w=420&sz=10&hl=en&start=1&um=1&itbs=1&tbnid=xFhT4l7IV0KfMM:&tbnh=83&tbnw=125&prev=/images%3Fq%3Dpictures%2Bof%2Blenin%2Bbody%26um%3D1%26hl%3Den%26biw%3D1276%26bih%3D540%26tbs%3Disch:1
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    The beginnings of the art and

    techniques of embalming are

    associated principally with ancient

    Egypt.

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    An accidental unearthing of natural mummy possibly ledthe Egyptian to firmly believe in an afterlife.

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    The ultimate punishment for the living, according to

    Egyptians was not living in hell, but ceasing to exist!!

    The heart of the dead person was weighed against "thefeather of truth," a symbol of Ma'at (the goddess of truth,

    justice, and order).

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    The heart was not taken out of the body because itwas the centre of intelligence and feeling and theman will need it in the afterlife.

    LiverLungsIntestines

    Stomach

    Imsety

    Hapy

    Duamutef

    Qebehsenuef

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    A long hook was used to smash thebrain and pull it out through the nose.

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    After the organs were removed, the body was washedwith wine and rubbed with spices. The alcohol in the wine

    acted as an antiseptic, helping to kill bacteria.

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    The corpse was then covered with natron for40 days to dry out.

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    Finally 68th to the 70th day----coffined.

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    Middle Ages(Era of Anatomists)During middle ages, there were increasing

    number of those who wanted to study the humanbody and preservation was no longer for thepurpose of an after-life but for study purposes.

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    Crusades period (1095 to 1291)

    There were series of wars & no preservationtechnique was developed.

    The procedure included removal of soft tissues

    and then boiling the bones until they were free ofall soft tissues.

    The bones were then dried and wrapped within

    bull hides and returned to their homeland for finalrites.

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    William Harvey (1578-1657)

    Embalming by arterial injectionas a mortuary practice was

    developed after Harveys

    discovery of circulation.

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    The English word ---

    to put on balm (spices & perfumes).

    EMBALMINGPerfusion of the cadaver withfixative fluid for the purpose

    of preventing putrefaction.

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    In the middle ages embalming became an art.

    Dr. Frederick Ruysch

    (1665-1717)

    His secret formula wasBalsamic spirit.

    1 pound of tartar dissolvedin 6 pounds of water, towhich he added half poundof sal ammoniac.

    After filtration it was readyfor embalming.

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    Hunter Brothers

    Hunter brothers were permitted to be sole agency forembalming and for performing anatomical dissections in thecity of London. William Hunter and John Hunter injected thefemoral artery with a solution composed of oil of turpentine,oil of lavender and vermilion dye.

    1718-1783 1728-1793

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    This is the scene from a battlefield showing theembalmer searching for cadavers. He would ignorethe cries of the injured, but look for dead bodies that

    he had been paid to embalm.

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    The modern embalming is defined as the

    study and science of treating a deadhuman body to achieve antisepticcondition, premortem appearance andpreservation.

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    The Embalming process acts on the body proteins.

    It changes the colloidal nature of proteins and forms a

    lattice-work of inert, more stable, longer-lasting and

    firm substance that can no longer serve as food for

    bacteria.

    This new protein form cannot be broken down by the

    enzymes from body cells or bacteria.

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    The process of embalming consists

    fundamentally of an injection of some

    suitable disinfecting preservative (arterial

    fluid) into the vascular system augmented

    by the relieving of the blood from the veins.

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    Receipt of the Dead Body

    In the Anatomy Department the dead

    body is received as1. Unclaimed Body

    2. Donated Body

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    Most important is legal documentation

    Identification of the body. Consent. Death certificate.

    No objection from police.

    Clearance from the embassy.

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    NOW WE ARE READY TO

    EMBALM

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    Transferthe body on the Embalmingtable.

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    Removeclothing frombody and shave hair.

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    Wash andDisinfect the body.

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    Size of vessel-should be sufficiently large to affordproper insertion of cannula.

    Depth of location of vessel-superficial enough toavoid unnecessary dissection.

    Proximity to the heart- In unautopsied body,vessels should be close to both aorta and right

    atrium.

    Special cases of infants - vessels of choice areabdominal aorta and inferior vena cava.

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    ARTERIES VEINS

    Right femoral artery

    Right common carotidartery

    Both right and leftcommon carotidarteries

    Right axillary artery

    Femoral vein

    Internal jugular vein

    Right atrium ,using atrocar (in difficultcases)

    Vessels of right side of thebody are commonly used ???

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    ARTERIES VEINS

    Abdominal aorta isused directly forinjection.

    Inferior vena cava

    Other sites

    Right and left common carotid arteries

    Right and left femoral (external or common illiac) arteries

    Right and left subclavian (axillary) arteries

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    Most commonly used artery for injection is

    Femoral Artery.

    And most common vein for drainage is FemoralVein.

    But there are other sites also..

    Common carotid artery and internal jugular vein

    Axillary artery and axillary vein

    Radial artery

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    After exposure pass two ligatures under theartery.

    Use a scalpel to open the artery.

    Now cannulate the artery towards the

    heart.

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    Transverse Diagonal T-shaped Wedge-shaped

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    Arteries are empty after death, therefore,

    best suited to carry the fluid to the tissues.

    They do not collapse when cut.

    Veins are large, thin walled with valves,

    usually engorged after death and collapse ifempty.

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    The hooks areused mainly inretraction ofskin andsuperficial fascia

    In raising theartery or a vein

    to the surfaceof the skin forarterialinjection.

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    IN UNAUTOPSIED BODIES IN AUTOPSIED BODIES

    One point injection

    Split injection

    Multipoint injection

    (in case of failure of

    one point injection

    technique)

    Six point injection

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    Done by embalming machines.

    Gravity method

    Electric pump method

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    Traditional, simplest, most commonlypracticed and least expensive method .

    It requires a graduated glass bottle of the

    capacity of ten litres with an outlet with a Tconnection which leads to two rubber tubingsconnected to two cannula.

    One Cannula is directed downwards and the

    other upwards.

    The T connection contains a screw clamp tocontrol the rate of flow of the fluid.

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    After the cannula is secured, theembalming bottle is filled with the arterialfluid and kept at a height of 4-6 feet from

    the embalming table.

    By the pressure of gravity the fluid startsflowing

    Complete injection takes nearly 7-8 hrs

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    ADVANTAGES DISADVANTAGE

    This can be asubstitute when there

    is a electric powerfailure.

    This method provides

    a slow, steady andmore thoroughdistribution of fluid.

    The method is timeconsuming and needsconstant refilling ofthe bottle.

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    ELECTRIC PUMP METHOD

    Simple device wherepressure is generated toforce fluid from an

    injection tank into thevascular system.

    Duration it delivers 8-10 liters of arterial fluidwithin 30-45 min.

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    Very high pressure should not be usedas it can rupture the arterial walls.

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    Through veins.

    Blood is present in large amounts inveins at the time of death..

    Contents of drainage?

    Purpose of the drainage?

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    Methods of drainage Advantages Disadvantages

    Alternate Thorough distributionof fluid

    More time consuming

    Concurrent(continuous) Less time consuming Incomplete Distributionof fluid

    Intermittent Less time consumingwith thoroughdistribution of fluid

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    Golden Rule for Drainage:

    Good drainage can be expected

    only when the interval between

    Death and Embalming

    is short.

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    Of proper and complete embalming:

    Skin looses elasticity.

    Face becomes fixed.

    Fluid oozes out from orifices.

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    Arterial embalming- arterial fluid is injectedinto selected artery.

    Cavity embalming- involves two steps:

    Aspiration of the cavities and their contents.

    Injection of a strong preservative chemical.

    If the cavity treatment is not donethe continuedactivity of the bacterial flora, already exist in theviscera, will promote putrefaction and ultimatelycause failure of embalming.

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    Hypodermic embalmingusing hypodermicneedle.

    Surface embalmingusing cotton packssoaked in arterial or cavity fluid.

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    What is an embalming fluid??

    A disinfectant?

    A preservative?

    Will it prevent contamination?

    Produces life like appearance?

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    Preservatives

    Germicides

    Buffers

    Wetting agents

    Anticoagulants

    Dyes

    VehiclePerfuming agents

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    Formalin/ formaldehyde -

    Discovered in 1856 by August

    Wilheld Von Hofmann.

    It is 37% by weight or 40% by

    volume of formaldehyde gas in

    water.

    Methyl alcohol acts as

    stabilizer (about 10% is

    added).

    August Wilhelm vonHofmann

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    It is a bactericidal but not aneffective fungicidal and insecticidal.

    Other preservatives

    Methanol and Phenol ..

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    Germicides -

    Eg:Phenol and its derivatives, zephiran

    chloride and glutaraldehyde.

    Buffers

    Assist in stabilizing the pH and providegood medium for reaction of

    preservatives with cellular proteins.

    Eg. Sodium borate/ Bicarbonate/Carbonate and magnesium Carbonate

    act as neutralizer of formalin.

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    Wetting agents-

    are used to lower the surface tension andsubsequently to facilitate the penetrationand distribution of embalming fluid throughvascular bed.

    Eg. Glycerin, sorbitol and sodium

    lauryl sulphate.

    Anticoagulants-Eg. Sodium Citrate and oxalate.

    D Si l h l l i f h

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    DyesSimulates the natural coloring of thetissues.

    Also enable the embalmer to detect patchyembalming effects if any.

    Eg.Eosin

    Vehicles A solvent that help the

    ingredients in the solution in a stable anduniform state during transport through thevascular system to different parts of the

    body.Eg. Water

    PerfumesEg.Methyl salicylate

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    Muscle relaxants-

    Eg.Magnesium chloride

    Recent view- relaxing agents are ineffective

    in a cadaver.

    But what about quantity of fluid???

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    CHEMICALS

    PURPOSE

    AMOUNT (for 1 lit.)

    FormalinMethanol Preservative 10.0%55.0%Sodium borate Buffer 15.0%Sodium citrate Anticoagulant 15.0%Glycerin Wetting agent 15.0% (20%*)Phenol Germicide 5.0%Water Vehicle 15.0% (10%*)Thymol (dissolve inalcohol) Fungicide Few crystals1% eosin Dye 5.0mlMethyl salicylate Perfume 10.0ml

    (For 1 ltr) increaseglycerine and decreasewater in Thin subjects*

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    CHEMICALS PURPOSE AMOUNTFormalinMethanol Preservative 60.0%25.0%Glycerin Wetting agent 2.5%Phenol Germicide 10.0%Mercuric chloride Insecticide and

    disinfectant 1.0%Lavender Perfume 1.0%

    (for 1 ltr fluid)

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    Used to keep the dissections moist.

    CHEMICALS PURPOSE AMOUNTGlycerin Wetting agent 75.0%Alcohol Preservative and

    to mix thymol10.0%

    Phenol Germicide 5.0%Water Vehicle 10.0%Thymol Fungicide Few crystals

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    For immersing the embalmed cadavers.

    CHEMICALS AMOUNTSFormalin 15.0%

    Glycerin 20.0%

    Phenol 5.0%

    Water 60.0%

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    To prevent drying of the isolateddissected parts.

    CHEMICALS AMOUNTFormalin 5.0%

    Glycerin 50.0%

    Phenol 5.0%

    Water 40.0%

    Thymol Few crystals

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    Use 10% formalin only.

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    Burnt bodies.

    Radiation bodies.

    Fetuses.

    Autopsied bodies.

    Diseased bodies like ascites, bed

    sores.

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    HIV infected bodies

    Treat the body with sodium hypochlorite(effective germicides)

    Consider all bodies are potentially

    dangerous with HIV (universal workprecautions) for minimizing the risk of

    exposure to blood and body fluids.

    (As per CDC ,Centers for disease

    control, recommendations).

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    Considerations for the health of

    embalmers.

    Considerations for disposal of human

    remains and other infected materials.

    Protective Equipment

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    Protective Equipment

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    The practice and purpose of embalminghas undergone a sea change over theyears and the guiding purpose hasshifted from predominantly cultural to

    educational.

    With advances in modern practice, we

    can look forward to further glorificationof the dead in future.

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    Thank you