JSM Dentistry

Cite this article: Helaly MN, Alam-Eldein AM, El-Sheikh AM (2018) Effect of Two Cleansing Agents on Color Stability of Two Thermoplastic Denture Base Materials. JSM Dent 6(2): 1110.

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*Corresponding authorMahmoud Nabil Helaly, Department of Prosthesis, Faculty of dentistry, October 6 university, Egypt, Tel: 01090643380; Email:

Submitted: 13 August 2018

Accepted: 29 August 2018

Published: 31 August 2018

ISSN: 2333-7133

Copyright© 2018 Helaly et al.

OPEN ACCESS

Keywords•Polyamide; Cleansing agents; Color stability;

Spectrophotometer

Research Article

Effect of Two Cleansing Agents on Color Stability of Two Thermoplastic Denture Base MaterialsMahmoud Nabil Helaly1*, Ahmed Mohammed Alam-Eldein2, and Ali Mohamed El-Sheikh21Department of Prosthesis, October 6 University, Egypt2Department of Prosthesis, Tanta University, Egypt

Abstract

Aim: Some patients experience adverse reactions to acrylic resin (PMMA) denture base material. Polyamide (PA) as an alternative to PMMA has however not been well documented regarding color stability. The aim of this in vitro study was to evaluate the effect of two different cleansing agents (corega and fitty dent), on color stability of two thermoplastic denture base materials (vertex thermosens and breflex).

Materials and methods: A total of 60 samples were fabricated from two different thermoplastic denture base materials (vertex thermosens and vreflex). The samples were divided into 3 groups. Each group was including twenty samples, ten samples each for vertex thermosens and 10 from breflex denture base materials. Samples of Group I were subjected to the distilled water as a control group. While samples of Group II were subjected to the corega as a cleansing agent and samples of group III were subjected to fittydent as the other cleansing agent. Effect of the two cleansing agents on the two-different thermoplastic denture base materials were evaluated and compared with regards to color stability.

Results: Regarding (vertex thermosens and breflex) both materials were relatively color stable in the two cleansing agents. “corega had less effect on color change than fittydent)

Conclusion: Corega and fittydent cleansing agents could be used safely for disinfecting denture base materials (vertex thermosens and breflex) as they had non-significant effect on color stability.

INTRODUCTIONMany material have been used for denture base construction

as acrylic resin (PMMA), nylon, poly either, etc. Unnecessary if acrylic should be mentioned at all, it should only be used to introduce the thermoplastic materials as an alternative [1].

Nylon is an alternative to acrylic resin as a denture base material and is a generic name for certain types of thermoplastic polymers known as polyamides. Nylon is a crystalline polymer, but PMMA is amorphous. This crystalline nature has a little liability to changes in physical and mechanical prosperities as has a few inter structural spaces for entrance of water, saliva and cleansing agents [2].

Color change within the denture base (PMMA) may be caused by the oxidation of the amine accelerator or by the penetration of colored solutions (nylon and PMMA). Most materials used for prosthetic treatment are subject to sorption, a process of absorption and adsorption of liquids dependent on environmental conditions [3]. Color change may be associated with porosity caused by overheating or insufficient pressure during polymerization or to excessive residual monomer, surface characteristics and microporosity in the specimens [3]. Staining of acrylic resins can lead to poor esthetics [3].

Color change of denture base materials may result in patient dissatisfaction and additional expense for replacement. Determination of color by visual means is considered highly subjective [4]. Methods used to assess changes in shade include digital analysis, projection of photographic slides, visual group ranking, and shade guide matching [4].

The spectrophotometer evaluates visible light through the color spectrum (400-700 nm) and expresses the analysis as a numerical value and is accurate method and avoids the human errors in visual methods [5]. Disinfection method should be effective without detrimental effects on the properties of materials used for fabrication of denture base. Everyday use of denture cleansers is recommended to prevent microbial colonization on denture and promote good oral health. Daily use of denture cleansers can affect the color stability of denture base material. In choosing a disinfectant for a dental prosthesis, consideration should be given to its compatibility with the type of material to be disinfected to avoid adverse effects [6,7].

MATERIALS AND METHODSIn this in-vitro study, a total of 60 samples were made from

two different thermoplastic denture base materials (vertex

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thermosens, Vertex-Dental B.V. Headquarters Netherlands) and (breflex, bredentWeissenhorner Germany). The samples were divided into 3 groups (Figure 1).

Preparation of metal discs

Metal disc shape patterns for water sorption and water solubility (50 ± 1 mm in diameter and 0.5 ± 0.05 mm) thick were fabricated from a private engineering works by laser cutting according to ADA specification no. 12. These metal disks were used to prepare the samples for the two denture base materials [8] (Figure 2).

Preparation of samples

Samples of this study were prepared according to the manufacture instructions by injecting thermoplastic materials into special flasks with holes to receive the capsule that contained the powder of thermoplastic material [9]. The flask filled with plaster then the metal disk was put in the flask to make a mould. The sprue of the soft wax was applied to the injection channel of the flask contained the sample metal disk impeded in the plaster.

The two halves of the flask were assembled and fastened with screws. The plaster was let to harden. The flasks were placed for 10 minutes in 70ºC water to soften the wax. The flasks were opened, and the wax were removed and cleaned with boiling water. The plaster was separated with Thermo-Flow isolation. The flasks were preheated for 15 minutes in ≥ 90°C water and were put in the machine just before injection. The injection with 6.5 bars of pressure was started after 16 minutes of preheating of the cartridge at 250ºC. The complete flasks were placed in an oven (>100ºC) or in boiling water for 30 minutes. In order to achieve optimal quality of the material, the flasks were bench cooling for 20 minutes and it was then opened.

The samples were taken carefully, and the injection channels were cut off. The edges were ground with the cross standard bur. The surface was polished with silicone polishers. Thermo-Gloss and a microfiber polishing brush were used for finishing. The final finish was completed with the brush felt cloth.

Color stability evaluation

The spectrophotometer and the International Commission

on Illumination (CIE) concept were used to carry out this test by measuring the light absorption of each specimen before and after immersion into corega and fittydent cleansing agents [10-12].

The apparatus used in measurements was AgilentCary5000spectrophotometers. It was manufactured according to a quality management system certified to ISO 9001.1

The basic CIE concept is that all colors can be matched by mixing relative amounts of the three light primaries: Red (X), Green (Y), and Blue (Z) [10]. These can then be transformed to L* is a measure of lightness. The a* value represents positions on a red-green axis. As a* becomes more positive in value, the color is more red; as a* becomes more negative in value, the color becomes more green. The b* value represents positions on a yellow– blue axis. As b* becomes more positive in value, the color becomes more yellow; as b* becomes more negative in value, the color becomes more blue [10].

The equation utilized for calculating color differences in this system10:

ΔE (Color Changes Value) = {(ΔL)2 +(Δa)2+(Δb)2}1/2

where ΔL, Δa and Δb are the differences in L, a and b values before and after immersion at each time interval. For this study, the ΔE result is used for analysis.

National Bureau of Standards (NBS) is used to quantify the color change. Critical marks of color difference according to NBS are shown in Table 1. Following formula is used to express NBS units.

NBS unit=ΔE x 0.92, Where ΔE stands for color change.

The samples were fixed to the spectrophotometer holder and the machine was turned on then the samples were tested.

All samples were evaluated for color stability before and after immersion in cleansing agents and water to evaluate the effect of cleansing agents (Figure 3 and 4).

RESULTS

Color stability

1 Cary 5000 Spectrophotometer provided from Agilent Technologies(USA).

Figure 1 The samples into three groups.

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Figure 2 Metal disk.

Figure 3 Samples.

Figure 4 Agilent Cary 5000 spectrophotometer.

Figure 5 Chart of the mean values of ΔE for different groups.

Figure 6 The bar chart of the mean values of color changes for different groups in NBS units.

Comparison of color stability of the Thermoplastic denture base material in different cleansing agents: The results of comparison between the different groups as regarding color change are shown in Table 2 and Figure 5.

The values of the color changes (ΔE) of vertex thermosens when immersed in water, corega and fittydent in comparison showed no significant difference (P-value = 0.9425).

The values of the color changes (ΔE) of breflex when immersed in water, corega, fittydent in comparison showed no significant difference (p-value =0.072).

Comparison of color change of the Thermoplastic denture base materials utilizing different cleansing agents using National Bureau of Standards (NBS)

The mean values of color change expressed in NBS units are shown in Table 3 and Figure 6.

The results revealed that all the groups showed slight change in color as interpreted Critical remarks of color difference with NBS values ranging from 0.5-1.5indicating slight color change.

DISCUSSIONNylon with relatively low water sorption levels and melting

points, was developed to overcome problems with earlier forms of denture base materials [8]. It was modified by reinforcement with glass fiber and glass spheres to increase its potential use as a denture base material. This modified material showed color stability [8].

Color stability has a long term effect on the esthetics of the denture which is a very important requirement for patient satisfaction [13]. Water sorption and solubility affect the

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mechanical properties and the adaptation of the denture base to the underlining structure. Using of cleansing methods for denture hygiene maintenance is mandatory [13], but cleansing methods unfortunately affect the physical properties of the denture base materials [13].

The vertex thermosens and breflex were used as a denture base materials in this study. These new materials have long chains inmolecular arrangement, that decrease the liability for water molecules to inter an desrubt the chemical structural of the denture base material which affect the color stability of the denture base material, Therfore improving the mechanical and chemical properties of the denture base materials [14,15].

Contaminated dentures need to be cleansed as part of denture hygiene regimen [16]. Chemical disinfection may cause adverse effect on the material, such as denture staining and compromised strength. To avoid adverse effects on the properties of denture base resins, disinfectant must be chosen that should have compatibility with the type of material to be disinfected [16].

The whitening effect relates to the high temperature of the water used in the solution. Irregularities and porosities present on denture surfaces offer a favorable niche to retain stain and microbial plaque [13,14].

A spectrophotometer was used to detect the color changes, which is a widely used digital image processing method for the denture base color change test because it can rule out errors due to observer bias. This system is recommended for material color difference tests because it is the most scientific color system [17-19]. Studies reported that extrinsic factors are more frequent causes of denture base color changes than intrinsic factors [20,21].

Hu et al. [22], Investigated the pigmentation of resin materials induced by absorption or adsorption. They found that denture base materials showed color stability in water or air, but there were changes in color stability when immersed in beverages such

as coffee, cleansing agents, tea, or wine. The tannic acid contained in coffee and tea are water-soluble and are known to trigger brown pigmentation.

Denture cleansers cause loss of soluble component and plasticizers from the denture base resins. Higher ionic concentration of denture cleansers compared to water led to a higher release of soluble components. Further absorption of water and other salivary components by the denture base materials leads to surface roughness and color change [23,24].

In the study of Lai et al. [25], The color stability of copolyamide, silicone and heat-polymerized acrylic resins as removable gingival flange materials were evaluated by a spectrophotometer after 7, 14, 30, 120, and 180 days of immersion in staining solutions of coffee and tea. Copolyamide had the greatest staining in tea solution and the silicone material in coffee solution. The color changes of silicone and copolyamide materials stored in coffee solution for 180 days were greater than 3 NBS (National Bureau of Standards) units, which would be characterized considerable and deliberated clinically unacceptable.

Takabayashi [26], Compared the color stability of six thermoplastic denture resin materials (three polyamides, two polycarbonates and a polyethylene terephthalate) after being soaked in coffee and curry solutions for 60 hours. In that study, three polyamides had considerable color change in the curry solution and two of them showed considerable color change after soaking in the coffee solution.

It was found that the frequency of amide groups along the chain had affected the color stability, water sorption and the chemical properties of each type of nylon. Another attributed reason could be the differences in finishing and polishing of nylon materials compared to PMMA. Rougher surfaces are more susceptible to staining [27,28].

The effects of three sodium perborate-containing denture cleansers (Corega, PR-Protefix, VA-Valclean) were evaluated on the surface roughness, hardness, and color stability of two polyamides (Valplast and Deflex), a butadiene styrene copolymer PMMA, and a PMMA polymer as a control group. Surface roughness of the polyamide increased after 20 days of repeated immersion regardless of the type of the solution used. Valplast had a higher initial surface roughness which increased after the immersion. This study also showed that no changes occurred in the color of polyamides in these solutions.

The study evaluated the effects of two denture cleansing methods [Val-Clean (peroxide cleanser) and Corega plus microwaving] on 3-D surface roughness, gloss and color of

Table 1: NBS: National Bureau of Standards.

Critical remarks of color difference NBS units

Trace 0.0-0.5

Slight 0.5-1.5

Noticeable 1.5-3.0

Appreciable 3.0-6.0

Much 6.0-12.0

Very much 12.0≥

Table 2: Mean values, SD, and comparison for color changes between different groups.Cleansing agentsWater Corega Fittydent

Thermoplastic denture base materials Mean SD Mean SD Mean SD p-value

Vertex thermosens 1.019 0.072 1.075 0.0987 1.094 0.0148 0.9425

Breflex 0.989 0.086 1.053 0.115 1.119 0.0916 0.0719

Difference -0.034 0.0865 -0.025

p-value 0.9153 0.106 0.711

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nylon (Valplast), and heat-polymerized acrylic PMMA denture base material for a period simulating 30 days of daily cleansing, by using an interferometric profilometer, a gloss meter, and a colorimeter. The results of this study showed that cleansing methods had no different effect on color group compared with the control group, when using the same material. However, the effect on Valplast was higher than PMMA, both at a clinically perceptible level.

The Val-Clean method was the only method that had no particular influence on the gloss of both tested materials. Corega plus microwaves significantly decreased the gloss of both materials. Surface roughness was affected significantly only by Corega plus microwaves and only for the PMMA material. The color change (as an effect of cleansing agent), was not associated with gloss or surface roughness in any of the materials. However, gloss and surface roughness were highly associated in PMMA and could be used for the prediction of each other

The present study showed that fittydent cleansing agent had the highest value of color changes when compared to water and corega, but the differences were insignificance and this may be due to that fittydent cleansing agent contain potassium mono sulfate and sodium bicarbonate which not contained in corega that may react with the coloring agents of the denture base materials used in this study not only that but also may increase the surface porosity.

CONCLUSION1- Based on the results of this study, we can conclude

thaCleansing agents had no noticeable effect on the color stability of the recent denture base materials (vertex thermosens and breflex).

2- Non significant differences were found between vertex thermosens and breflex denture base materials with the use of different cleansing agents (corega, fittydent, water) regarding color stability.

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Table 3: The mean values of color change for different groups in NBS units.

Vertex thermosens Breflex NBS Units

Water 0.9373972 0.90988

Slig

ht (

0.5

-1.5

)

Corega 0.9891104 1.02948

Fitty Dent 1.00602 1.02948

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Helaly MN, Alam-Eldein AM, El-Sheikh AM (2018) Effect of Two Cleansing Agents on Color Stability of Two Thermoplastic Denture Base Materials. JSM Dent 6(2): 1110.

Cite this article

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Effect of Two Cleansing Agents on Color Stability of Two Thermoplastic Denture Base MaterialsAbstractIntroductionMaterials and Methods Preparation of metal discs Preparation of samples Color stability evaluation

ResultsDiscussionConclusionReferencesFigure 1Figure 2Figure 3Figure 4Figure 5Figure 6Table 1Table 2Table 3