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alzheimers disease, AD 90 SD 15 - 1-42(A1-42) AD 1 0.81.6 3.2 g/kg 0.02 mg/kg 30 dSODGSH-PxMDA [(239.0548.42)s(214.3574.52)s (97.3930.69sP 0.01][(1.933.25)(2.273.09)(6.623.45)P 0.05]SOD[(177.2763.10)U/mg(164.5372.58)U/mg (72.5621.04)U/mgP 0.01] GSH-Px [(2 899.36362.27)U/g(2 407.68472.14)U/g (1 397.64442.17)U/gP 0.01]MDA [(24.759.94)nmol/mg(27.745.82)nmol/mg (37.5617.23)nmol/mgP 0.01] AD

Effects of Tianqi-Yizhi granules on oxidative stress in the brain tissue, and learning and memory in Alzheimer's disease model rats Zhao Jun*, Wu Yiming, Ma Tao, Wei Dongfeng. *Worker Hospital of Xuanhua Steel Company, Hebei Iron & Steel Group, Zhangjiakou 075100, China Corresponding author: Ma Tao, Dongfang Hospital Affiliated to Beijing University of Chinese Medicine, Beijing 100078, China; Email: matao327@126.com

Abstract Objective To investigate the effects of Tianqi-Yizhi granules on oxidative stress in the brain tissue, and learning and memory in Alzheimer's disease model rats. Methods A total of 90 male SD rats were randomly divided into 6 groups by random number table method: sham operation group, model group, huperzine A group and groups of low-, medium- and high-dose Tianqi-Yizhi granules, with 15 rats in each group. The AD rat model was prepared by the left lateral ventricle injection of amyloid-1-42. One week after modeling, the rats in the groups of low-, medium- and high-dose Tianqi-Yizhi granules received intragastric administration of 0.8, 1.6 and 3.2 g/kg Tianqi-Yizhi granules, respectively; the rats in the huperzine A group received intragastric administration of 0.02 mg/kg huperzine A solution; and the rats in the sham operation and model groups received intragastric administration of equivalent volume of normal saline for 30 days. Learning and memory were evaluated using the dark avoidance test. The activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), and malonaldehyde (MDA) level in the brain tissue were determined. Results In comparison with the model group, the latencies to step into the dark chamber in the high- and

DOI: 10.3760/cma.j.issn.1673-4246.2015.03.013

2013ZX09103002-00281430100 075100 100078

100875 Email: matao327@126.com

240 20153373 Int J Trad Chin Med, March 2015, Vol. 37, No.3

medium-dose Tianqi-Yizhi granules groups were significantly longer (239.05 48.42 s, 214.35 74.52 s vs. 97.39 30.69 s; all P

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A1-42 AD 1 3.21.60.8 g/kg 0.02 mg/kg 30 d 1.6 2 h 2 1 5 min 24 h 1.7 RIPA 4 10 000 r/min BCA SODGSH-PX MDA 1.8 SPSS13.0 sx one-way ANOVA Post Hoc P0.05

2 2.1 30 dP0.01

P0.05 P0.01P0.05 1 1 ( sx )

(mg/kg) (s)

()

15 276.3459.29 0.731.21 15 97.3930.69a 6.623.45a 15 0.02 225.8269.03b 1.723.90c

15 3 200 239.0548.42b 1.933.25c

15 1 600 214.3574.52b 2.273.09c

15 800 194.2133.04b 5.432.32

aP0.01bP0.01cP0.05 2.2 SODGSH-PX MDA P0.01SODGSH-PX MDA P0.05 P0.01 2

3

A1-42

2 SODMDAGSH-PX ( sx ) (mg/kg) SOD (U/mg) MDA(nmol/mg) GSH-PX (U/g)

6 243.2647.65 22.63 8.86 3 572.12543.29 6 72.5621.04a 37.5617.23a 1 397.64442.17a 6 0.02 146.0354.04b 33.4214.05c 1 647.05329.77 6 3 200 177.2763.10b 24.75 9.94b 2 899.36362.27b 6 1 600 164.5372.58b 27.74 5.82b 2 407.68472.14b 6 800 132.4969.14c 35.05 9.43c 2 409.39448.93b

SODMDAGSH-PXaP0.01bP0.01cP0.05

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A1-42 AD

AD AD AD [5] SODGSH-PX [6] AD A AD A A1-40 A1-42 A1-42 A AD A [7]A [6-7] A AD [8-9] A1-42 AD

[3-4] AD

AD

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