e n z y m glucoamylase se - sekisuidiagnostics.com · origin rhizopus sp. specifications appearance...
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O R I G I N Rhizopus sp.
� S P E C I F I C A T I O N SAppearance White Lyophilised PowderActivity ≥30 U/mg powder at 40°C
� A P P L I C A T I O NUsed in the determination of alpha Amylase.
� U N I T D E F I N I T I O NOne unit causes the formation of ten milligrams of glucose in 30 minutes at 40°C under standard assay method conditions.
� A S S AY P R I N C I P L EGlucoamylase catalyses the following reaction:
Starch + nH2O n Glucose + Dextrin
The formation of glucose is measured as a reducing sugar by the modified Fehling-Lehmann-School Method.
GlucoamylaseE N Z Y M E S
CAT# G
LUC-70-6881
EC# 3.2.1.1.2
C A T # G L U C - 7 0 - 6 8 8 1E C # 3 . 2 . 1 . 1 . 2
EN
ZY
ME
S
Glucoamylase
Molecular Weight: ~70kDa
Kmvalues: Maltose 11 ± 1.1 x 10-4 MMaltotriose 3.6 ± 0.51 x 10-4 MMaltotetraose 2.5 ± 0.33 x 10-4 MMaltopentaose 1.6 ± 0.02 x 10-4 M
Optimum pH (Fig. 1): 4.5 to 5.0
Optimum Temperature (Fig. 2): 60°C
pH Stability (Fig. 3): 4.0 to -8.5 (25°C for 20 hours)
Thermal Stability (Fig. 4): Stable at 45°C and below (pH 5.5 for 10 minutes)
Substrate Specificity: Hydrolyses soluble starch amylopectin, glycogen, α- or β- limit dextrin, amylose, maltooligosaccharides, and panose.
� C H A R A C T E R I S T I C S
CAT# G
LUC-70-6881
EC# 3.2.1.1.2
EN
ZY
ME
SRELATIVE ACTIVITY (%)
pH
120
100
80
60
40
20
0
20 30 40 50 60
F IGURE 1 : OP T IMUM pH
F IGURE 4 : pH S TAB I L I TY(pH 5 . 5 FOR 10 M INUTES )
F IGURE 2 : OP T IMUM T EMPERATURE
F IGURE 3 : THERMAL S TAB I L I TY(25 °C FOR 20 HOURS )
RELATIVE ACTIVITY (%)
TEMPERATURE (°C)
120
100
80
60
40
20
0
50 60 70
RESIDUAL ACTIVITY (%)
pH
120
100
80
60
40
20
0
3 4 5 6 7 8 9 10
RESIDUAL ACTIVITY (%)
TEMPERATURE (°C)
120
100
80
60
40
20
0
42 44 46 48 50 52 54 56 58 60
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