Yeast extract for Yeast extract for FermentationFermentation
Xiao Li Ph. D Angel Yeast Co.,
Ltd4.19.2014(In
Vietnam)
Contents
Applications Applications ofof organic nitrogen sources for fermentationorganic nitrogen sources for fermentation
AdvancesAdvances in in modern fermentation regulation technologymodern fermentation regulation technology
1
3
2
Part 1 ComparisonComparison of of typical organic nitrogen source typical organic nitrogen source
B . . Comparison with different organic nitrogen Comparison with different organic nitrogen sourcessources
A . Summary of organic nitrogen sourceSummary of organic nitrogen source
A . Summary of Summary of organic nitrogen source organic nitrogen source
yeast exact
yeast peptone
beef extract(powder)
corn steep liquor corn steep liquor (powder) cottonseed powder
yeast powder
soybean powder peanut
powder
Category
others
Microbe sources
Plant source
sAnimal sources
tryptone
Plant sources
Other sourcesOther sources
A . Summary of Summary of organic nitrogen organic nitrogen sourcesource
Animal sources
corn steep liquor :corn steep liquor : by-product of corn starch production ; made from corn soaking water with sulfite after concentration
cottonseed meal: oil manufacture by smashed cottonseed cakes
beef extract:fresh beef is processd with eliminating fat, hydrolysis, filtration,ConcentrationTryptone: casein hydrolyzed by Trypsin.
Microbe sources
YE : yeast processed by autolyze, enzymolysis, concentration and drying
Yeast powder: inactivated yeast; especially contains cell wall polysaccharides
Typical
organic organic nitrogen nitrogen
yeast exact(Microbe sources)
corn steep liquor(Plant sources)
beef extract(Animal sources)
Advantages
efficient;clean
Safety;
high bioavailability
rich in Phosphorus, proline, arginine, phyticacid(ex.:promote the fermentation of penicillin, gentamicin and also can promote the growth of lactobacillus)
rich in creatine, creatinine, probably has a postive effect on bacteria growth
Disadvantages
no reported at present
Not stable
Contain allergens
Not stable
Not safe
The advantages of yeast extract as nitrogen sourceThe advantages of yeast extract as nitrogen sourceThe advantages of yeast extract as nitrogen sourceThe advantages of yeast extract as nitrogen source
YE
In favor of In favor of cleaner cleaner
productionproduction
easy to easy to be be
utilizedutilized safetysafety
as been widely used as efficient microbial medium in fermentation field
comprehensive comprehensive and balanced and balanced
nutrientsnutrients
Fermentation EngineeringFermentation Engineering
Medicine 、 Food 、 Agriculture 、 Environmental protection 、 Energy field…Medicine 、 Food 、 Agriculture 、 Environmental protection 、 Energy field…
The important content and key technology in the field of medicine and food
FoodAdditives 、 preservative 、 Functional food
Animal cells culturenew drugs 、 vaccine 、enzymes……
Biological energy
cellulosic ethanol , biodiesel ……Environmental
protection:biological treatment 、 biological
forecast…
AgricultureDeep processing of agricultural products;Biological pesticide and fertilizer...
…………
Microbial medicineAntibiotics 、 vitamins 、GM products
Biochemicalsdegradable plastics 、 Bio-PE…….
Part 2 AdvancesAdvances in Modern fermentation in Modern fermentation
regulation technologyregulation technology
Metabolic Metabolic engineeringengineering
Research directions of Research directions of FermentationFermentation
Optimization Optimization of of
fermentation fermentation processprocess
Part 2 AdvancesAdvances in modern fermentation in modern fermentation
regulation technologyregulation technology
Part 2 AdvancesAdvances in modern fermentation in modern fermentation
regulation technologyregulation technology
A . Study on microbial metabolism(multi-scale research)A . Study on microbial metabolism(multi-scale research)
Gene scale
Cell scale
Engineering scale
Scale-up researchScale-up research
combined with computer analysis to achieve key regulation parameters
Part 2 AdvancesAdvances in modern fermentation in modern fermentation
regulation technologyregulation technology
Optimization of culture medium
Optimization of batch fermentation
Optimization of fed-batch
fermentation
Optimization of continous
fermentation
Study on the theory of Study on the theory of fermentation regulationfermentation regulation
B. Optimization of fermentation process
The role of nitrogen sources in fermentation processA.
B. Requestments for nitrogen sources in Microbial fermentation
C.C. Requestments for YEF in Microbial fermentation
Part 3 Part 3 Applications Applications ofof organic nitrogen sources for fermentationorganic nitrogen sources for fermentation
E.E. *Applications in different fermentation fields*Applications in different fermentation fields
RReasonable collocation of different nitrogen source easonable collocation of different nitrogen source in fermentationin fermentationD.D.
nitrogen source
Cell growthCell growth
organic nitrogen source :corn steep liquor 、 soybean powder 、 urea 、 *YE 、…
inorganic nitrogen :Ammonium sulfate 、 Ammonium nitrate 、 Ammonia 、…
carbon source : CO2↑
A. The role of nitrogen sources in fermentation process
Part 3 Part 3 Applications Applications ofof organic nitrogen sources for fermentationorganic nitrogen sources for fermentation
Productmetabolite 、 biotransformation 、
enzyme 、 biomass
Genetic engineering
vaccine products
Part 3. Part 3. Applications Applications ofof
organorganiic nitrogen sources for fermentationc nitrogen sources for fermentation
B. Requestments for nitrogen sources in Microbial fermentation
1. rich in quick-acting
nutrition
2. demand for Special nutritional
factors
1. rich in quick-acting
nutrition
2. demand for Special nutritional
factors
Primary metabolic products
Secondary metabolic products
1. Reasonable composition of quick and
slow-released nitrogen source
2. The limit of special
nutritional factors
1. Reasonable composition of quick and
slow-released nitrogen source
2. The limit of special
nutritional factors
1. Biological safety
2. Fastidious for nutrients
3.Little influence on followed extraction
1. Biological safety
2. Fastidious for nutrients
3.Little influence on followed extraction
Requestments for YEFin Primary metabolic products fermentation
Requestments for YEFin Primary metabolic products fermentation
Requestments for YEFin secondary metabolic products fermentation
Requestments for YEFin secondary metabolic products fermentation
Requestments for YEFin genetic engineering vaccine products fermentation
Requestments for YEFin genetic engineering vaccine products fermentation
Part 3. Part 3. Applications Applications ofof
organic nitrogen sources for fermentationorganic nitrogen sources for fermentation
C. Requestments for YEF in Microbial fermentation
quick-acting nitrogen source :AN and corn steep liquor could promote the biomass, but inhibit secondary metabolite synthesis.
Slow-release nitrogen source : soya bean powder extend the phase of secondary metabolite synthesis.
Part 3. Part 3. Applications Applications ofof
organic nitrogen sources for fermentationorganic nitrogen sources for fermentation
D. Cases:D. Cases: reasonable collocation of different nitrogen source reasonable collocation of different nitrogen source in fermentationin fermentationD. Cases:D. Cases: reasonable collocation of different nitrogen source reasonable collocation of different nitrogen source in fermentationin fermentation
Part 3. Part 3. Applications Applications ofof
organic nitrogen sources for fermentationorganic nitrogen sources for fermentation
E. E. Applications in different fermentation fieldsApplications in different fermentation fieldsE. E. Applications in different fermentation fieldsApplications in different fermentation fields
Biological vaccines
ProbioticsVinegar
Enzyme preparationsAntibiotics
Biological mask(bacterial cellulose
By Acetic acid bacteria
fermentation)
Fermentation Fermentation FieldsFields
Amino acid, organic acid, hyaluronic acid, nisin, Biomass energy …….
fermentation× 50L tank
5%;0.5-1VVM,500-700rpm,37℃
20-28h
Amplication fermentation× 60m3 、
× 200m3tank……
Part 3. Part 3. Applications Applications ofof
organic nitrogen sources for fermentationorganic nitrogen sources for fermentation
E.1 E.1 High cell density culture process of Bacillus subtilis Bacillus subtilis E.1 E.1 High cell density culture process of Bacillus subtilis Bacillus subtilis
Activated slant strains
Seed culturepH7.0,
37℃ , 14h225rpm
Shake flask (30/250ml)
inoculating 1 loop
Seed liquid culture
37℃ , 220rpm Shake flask
18-20h 200/1000ml inoculums: 5%
Optimum medium ,conditions , fed-
batch strategy
Achieve Achieve HCDC HCDC
E.1 E.1 High cell density culture of Bacillus subtilis Bacillus subtilis E.1 E.1 High cell density culture of Bacillus subtilis Bacillus subtilis
Part 3. Part 3. Applications Applications ofof
organic nitrogen sources for fermentationorganic nitrogen sources for fermentation
Seed medium: Peptone 5 g, yeast extract FM902 6g, NaCl 5g, water 1L.pH7.0—7.2.
The optimal fermentation medium (mainly used in research)Ⅰ : rice straw powder 200g, wheat bran 800g,supplemented with glucose 50g, Tryptone 20g, yeast extract FM808 20g, NH4NO315g, KH2PO410g, water, 1500g.. pH7.0—7.2.
The optimal fermentation mediumⅡ (mainly used in industry): glucose 40g/L, yeast peptone FP101 10g/L, yeast extract FM902 35g/L, KH2PO415g/L, CaCO3 25 g/L. pH7.0—7.2.
Part 3. Part 3. Applications Applications ofof
organic nitrogen sources for fermentationorganic nitrogen sources for fermentation
Drying process
Fermented broth
centrifuged
paste Powder mixed with
CaCO3 as feed
Spray drying
Fermented broth+dextrin
Spray drying Powder as feed
Fermented broth
centrifugedpaste +Bran+CaCO3 Powder
stoving
Part 3. Part 3. Applications Applications ofof
organic nitrogen sources for fermentationorganic nitrogen sources for fermentation
E.2. E.2. Vinegar fermentationfermentationE.2. E.2. Vinegar fermentationfermentation
(1) Raw material: rice vinegar, bran vinegar, fruit vinegar, wine vinegar, substitute material vinegar etc.
(2) vinegar fermentation process: Solid-state ; liquid fermentation ;solid-liquid fermentation
(3) color and luster of the product: Smoked vinegar; Pale vinegar; White vinegar
CategoryCategory
Part 3. Part 3. Applications Applications ofof
organic nitrogen sources for fermentationorganic nitrogen sources for fermentation
E.2. E.2. Vinegar fermentationfermentationE.2. E.2. Vinegar fermentationfermentation
Part 3. Part 3. Applications Applications ofof
organic nitrogen sources for fermentationorganic nitrogen sources for fermentation
(1) Traditional (1) Traditional Vinegar fermentationfermentation(1) Traditional (1) Traditional Vinegar fermentationfermentation
Part 3. Part 3. Applications Applications ofof
organic nitrogen sources for fermentationorganic nitrogen sources for fermentation
(2) Improved (2) Improved Vinegar fermentationfermentation(2) Improved (2) Improved Vinegar fermentationfermentation
Nutritive saltsNutritive saltsnutrientsnutrients
Part 3. Part 3. Applications Applications ofof
organic nitrogen sources for fermentationorganic nitrogen sources for fermentation
(3) Alcohol (3) Alcohol Vinegar fermentationfermentation(3) Alcohol (3) Alcohol Vinegar fermentationfermentation
Packing Packing
Cooling water tower Cooling water tower Cooling water inletCooling water inlet
Nutritive saltsedible alcohol
water
Nutritive saltsedible alcohol
water
charging stock tank charging stock tank FermentorFermentor Product mixProduct mix
FiltrationFiltration
discharge tank discharge tank
WarehouseWarehouse
Cooling water outletCooling water outlet YEWater
YEWater
Part 3. Part 3. Applications Applications ofof
organic nitrogen sources for fermentationorganic nitrogen sources for fermentation
Theory of producing Theory of producing Vinegar Theory of producing Theory of producing Vinegar
Part 3. Part 3. Applications Applications ofof
organic nitrogen sources for fermentationorganic nitrogen sources for fermentation
Theory of producing Theory of producing Vinegar Theory of producing Theory of producing Vinegar
Nutritive salts
in Vinegar fermentation
Part 3. Part 3. Applications Applications ofof
organic nitrogen sources for fermentationorganic nitrogen sources for fermentation
E.2. E.2. Vinegar fermentationfermentationE.2. E.2. Vinegar fermentationfermentation
The vinegar Nutritive salts contains essential salts and growth factor which could promote growth and metabolism for acetic acid bacteria and effectively shorten the fermentation period, increase production, improve the quality of acetic acid and reduce the cost of the production.
Acitic acid fermentation
ADYα-Amylase
GlucoamylaseNutritive salts YE
Part 3. Part 3. Applications Applications ofof
organic nitrogen sources for fermentationorganic nitrogen sources for fermentation
Provide product scheme to youProvide product scheme to you
ReferencesReferences
Part 3. Part 3. Applications Applications ofof
organic nitrogen sources for fermentationorganic nitrogen sources for fermentation
E.2. E.2. Vinegar fermentationfermentationE.2. E.2. Vinegar fermentationfermentation
1.Research on Optimization of Fermentation Medium for Alcohol Vinegar Production by Uniform Design( Key Laboratory of Industrial Biotechnology of Ministry of Education jiangnan University ,Wuxi 214122 , China)
ABSTRACT :The experimental data were analyzed by stepwise regression to obtain a linear regression model for optimized fermentation media: Glucose 5 g /L , yeast powder 3 g /L , NaH2PO4 0. 7 g /L , KH2PO4 0. 6 g /L , MgSO4 0. 4 g /L . Under these conditions , a batch fermentation conducted in 5 L tank for 25 h , which obtained 0.42 g /L of biomass , 5.76 g /100 mL acetic acid . After that , semi-continuous fermentation were acted , 95% conversion rate of ethanol . The productivity was 0. 2 g /( 100 mL·h) , which had achieved the international level of production of alcohol vinegar .
E.3. Lincomycin fermentation
Lincomycin, a member of the lincosamide group of antibiotics, is produced by Streptomyces lincolnensis, and it is used for the treatment of diseases caused by Gram-positive bacteria. At present, Lincomycin is produced by many factories at home and abroad, but the strain is easy to deteriorate, which would lead to the decline of production. Furhtermore, the biosynthesis route and regulation mechanism of lincomycin are still unclear, so it would be a complicated task to optimize and scale-up the fermentation process.
Part 3. Part 3. Applications Applications ofof
organic nitrogen sources for fermentationorganic nitrogen sources for fermentation
E.3. Lincomycin fermentation
Part 3. Part 3. Applications Applications ofof
organic nitrogen sources for fermentationorganic nitrogen sources for fermentation
E2. Lincomycin fermentation
Seed medium ( % ): Starch 1.4 , Glucose 2.8 , Soya bean powder
2.0 , FM802 2.6 , NaCl 0.07 , NaNO3 0.085 , CaCO3 0.7 , NH4NO3 0.2 , (NH4)2SO4 0.23 , KH2PO4 0.0045%, initial pH 7.3.
Fermentation medium ( % ) Starch 1.0 , Glucose 4.0 , Soya bean powder 2.78
, FM902 0.3 , Yeast power YP101 0.6, NaCl 0.52 , NaNO3 0.62 , KH2PO4 0.026 , CaCO3 0.74, NH4NO3 0.21 , (NH4)2SO4 0.21, initial pH 7.8.
Part 3. Part 3. Applications Applications ofof
organic nitrogen sources for fermentationorganic nitrogen sources for fermentation
E2. Lincomycin fermentation
Part 3. Part 3. Applications Applications ofof
organic nitrogen sources for fermentationorganic nitrogen sources for fermentation
E2. Lincomycin fermentation
Part 3. Part 3. Applications Applications ofof
organic nitrogen sources for fermentationorganic nitrogen sources for fermentation
in the early stage
Part 3. Part 3. Applications Applications ofof
organic nitrogen sources for fermentationorganic nitrogen sources for fermentation
vacuoles vacuoles
Hyphae were brokenHyphae were broken
Yeast extract FM902 was added to the broth, andμwas kept at about 0.02
In the mid-late and late stage In the mid-late and late stage
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