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What should we do
about the Japanese
Beetle?
SymROSEium March 9, 2019
Whitney Cranshaw
Colorado State University
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Japanese beetlePopillia japonica
Coleoptera: Scarabaeidae
First U.S.
detection – 1916
near Riverton,
New Jersey
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Distribution of
Japanese
beetle in
Colorado
based on CDA
trapping
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4
This is a map
of the JB
situation in
the Denver
metro area
ten years ago
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Japanese beetle adults
chew on leaves and flowers
of many plants
Japanese beetle
damages plants in
two distinct ways
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Japanese beetle larvae
(grubs) – among the most
damaging turfgrass insects in
the US
Japanese beetle affects
yard/garden plants in two
distinct ways
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White grubs prune the roots,
producing drought stress symptoms
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Skunk digging damage
associated with white grub
infestations
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Managing Japanese
beetle in the larval
(white grub) stage
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Recommendations for Japanese Beetle
Larval Control
•Cultural Controls
•Biological Controls
•Chemical Controls
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One resource.
The Extension
Fact Sheet on
Japanese
Beetle
management
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Recommendations for Japanese Beetle
Larval (Grub) Control
• Cultural Controls
–Promotion of root growth to tolerate
larval feeding– Allowance of some soil drying during critical JB life
stages
– Provision of water to allow tolerance and recovery
from root loss
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White grubs damage
turfgrass by feeding
on roots
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Grasses with larger
root mass are better
able to tolerate effects
of root pruning insects
Mowing height greatly
affects root mass of
turfgrasses!
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Recommendations for Japanese Beetle
Larval Control
• Cultural Controls– Promotion of root growth to tolerate larval feeding
–Allowance of some soil drying
during critical life stages– Provision of water to allow tolerance and recovery
from root loss
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Generalized Life History Sequence of Japanese Beetle
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Adults burrow into the ground to lay eggs. Eggs
are only laid in soil that is suitably moist.
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Eggs and 1st stage larvae are very sensitive to drying
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Recommendations for Japanese Beetle
Larval Control
• Cultural Controls– Promotion of root growth to tolerate larval feeding
– Allowance of some soil drying during critical JB life stages
–Provision of water to allow tolerance of and
recovery from root loss
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White Grub Larval Treatments
• Insecticides
– Imidacloprid (Merit, Hi-Yield Insect & Grub Control, Hi-
Yield Grub Control II, others)
– Clothianidin (Arena)
– Chlorantraniliprole (Acelepryn, Scott’s GrubEx)
– Trichlorfon (Dylox, Proxol, Bayer Advanced 24 Hour
Grub Killer Plus Granules)
• Biological Controls
– Heterorhabditis spp. parasitic nematodes
– Bacillus thuringiensis var. galleriae (grubGONE!)
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White Grub Larval Treatments
• Insecticides
– Imidacloprid (Merit, Zenith, Mallet, Bonide Insect & Grub
Control, Hi-Yield Grub Control II, others)– Clothianidin (Arena)
– Chlorantraniliprole (Acelepryn, Scott’s GrubEx)– Trichlorfon (Dylox, Proxol, Bayer Advanced 24 Hour Grub Killer Plus Granules)
• Biological Controls
– Heterorhabditis spp. parasitic nematodes
– Bacillus thuringiensis var. galleriae (grubGONE!)
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Imidacloprid for White Grubs
• Trade Names(Retail): Hi-Yield Insect & Grub
Control, Hi-Yield Grub Control II, Bayer Advance
Season Long Grub Control
• Neonicotinoid insecticide
– Moves systemically in plants
– Can have hazard to bees exposed through nectar, pollen
• Provides control for month or two
– Fairly slow moving in soil and into plants
Optimal time for application: Early period of egg hatch
– typically late June through midJuly
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Imidacloprid for White Grubs
• Trade Names(Retail): Bonide Insect & Grub Control, Hi-Yield Grub
Control II, Bayer Advance Season-Long Grub Control
• Neonicotinoid insecticide
– Moves systemically in plants
– Can have hazard to bees exposed through nectar, pollen
• Provides control for month or two
– Fairly slow moving in soil and into plants
Optimal time for application: Early period of
egg hatch – typically late June through
midJuly
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Systemic insecticides and Pollinators – Should we
be concerned about their use on turfgrass?
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A key risk to pollinators when
using insecticides on turfgrass
Application to
flowering weeds
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Mowing before application >greatly<
decreases hazard to pollinators!
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Chlorantraniliprole for White Grubs
• Trade Names: Acelepryn, GrubEx
• Anthranilic diamide insecticide
– Limited systemic activity
–Very low hazard to bees
• Relatively slow acting
• Provides control for months
Optimal time for application: Early period of egg hatch
– typically mid-late June through midJuly
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Chlorantraniliprole is an insecticide in the
anthranilic diamide class
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Ryania speciosa
Powdered stems are the source
of the insecticide ryania.
Active ingredient: ryanodine
Botanical source that lead to
development of the anthranilic
diamides
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Anthranilic Diamides
• Based on chemistry of active compounds in ryania
(ryanodines)
– Effects ryanodine receptors associated with insect muscle
• Some systemic activity in plants• Extremely low toxicity to mammals (Category IV)
• Recently registered products:
– chlorantraniliprole (Acelepryn)
– cyantraniliprole (Ference – turfgrass label only)
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Anthranilic Diamides
• Based on chemistry of active compounds in ryania (ryanodines)
– Effects ryanodine receptors associated with insect muscle
• Some systemic activity in plants
• Very low toxicity to mammals (Category IV)
• Recently registered products:– chlorantraniliprole (Acelepryn)
– cyantraniliprole (Ference – turfgrass label only)
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Chlorantraniliprole for White Grubs
• Trade Names: Acelepryn, GrubEx
• Anthranilic diamide insecticide
– Limited systemic activity
–Very low hazard to bees
• Relatively slow acting
• Provides control for months
Optimal time for application: Early period of egg
hatch – typically mid-late June through midJuly
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Recommendations for Japanese Beetle
Larval Control
• Cultural Controls
• Chemical Controls
• Biological Controls– Insect parasitic nematodes (Heterorhabditis spp.)
–Bacillus thuringiensis var. galleriae
–Milky spore
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Photograph courtesy of Peggy Greb
Insect Parasitic Nematodes (“Beneficial
Nematodes”, “Predator Nematodes”)
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Nematodes enter insects
through natural openings
Heterorhabditis spp.
nematodes can penetrate
directly through the body
wall
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Insect parasitic nematodes enter a
host insect and introduce bacteria
(Xenorhabdus spp.)
The bacteria kill the insect
The nematodes eat the bacteria and
the decomposed insect
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Grubs turn a reddish color when killed by Heterorhabditis nematodes
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White Grub Larval Treatments
• Imidacloprid (Hi-Yield Insect & Grub Control, Hi-Yield Grub
Control II, Bayer Advance Season Long Grub Control, Merit,
Zenith, Mallet, etc.)
• Chlorantraniliprole (Acelepryn, Scott’s Grub-Ex)
• Heterorhabditis spp. parasitic nematodes
• Bacillus thuringiensis var. galleriae/Btg(grubGONE!)
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BTG - Bacillus
thuringiensis var.
galleriae
New biological control
for Japanese beetle -
and other grubs?
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Milky Spore for Japanese Beetle?
Used to permanently establish a biological control
organism – not useful for immediate control.
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Lawn Aerator
Sandals (a.k.a.,
“Spikes O’ Death”)
White grub blast
from the past
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Spikes O’ Death In Action
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Question: Does control of larvae in a yard
affect the number of adults in a yard?
Answer: Very likely, NO
????????
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Corn earworm (adults)
Grasshoppers
Japanese beetle (adults)
Crucifer flea beetles
Potato/tomato psyllid
Some Highly
Mobile Insects
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Adult beetles feed on both
flowers and leaves of many
ornamental plants as well as
garden vegetables and herbs
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Skeletonizing injuries produced by
Japanese beetle adults feeding on leaves
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Flowers are often a favored plant
part targeted by adult Japanese
beetles
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Overlap of adult feeding on
flowers – and use of those
flowers by pollinators
Issue of unusual concern
with Japanese beetle
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Uber-host Plants Favored by Japanese
Beetle Adults in CO• Roses**
• Linden*
• Virginia Creeper*
• Silver lace**
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Other Plants Commonly Grown in CO that are Highly Favored
by Japanese Beetle
Ornamentals
• Hollyhock*
• Gaura**
• Rose-of-Sharon**
• Crabapple
• Japanese maple
• Peking cotoneaster
Food Crops
• Beans (green, edamame)
• Basil
• Raspberry*
• Grape
• * JB populations overlap with flowering
• ** JB populations overlap >alot< with flowering
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Evaluations of roses at the War
Memorial Garden in Littleton, 2016-2017
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Japanese Beetle Damage Evaluations on
Roses – War Memorial Rose Garden
• Seven observations were made during season
• Damage by Japanese beetle ranked on a 0 to 3 scale
(no damage to heavy damage)
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Japanese Beetle Damage Evaluations on
Roses – War Memorial Rose Garden
• Seven observations were made during
season
• Damage by Japanese beetle ranked on
a 0 to 3 scale (no damage to heavy
damage)
• Observed flower visitation
by bees ranked on a 0 to 3
scale (no visitation to high
visitation)
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Roses that Were Not Observed Damaged by
Japanese Beetle
• Child’s Play
• Cupcake
• Gemini
• Old Glory
• Rainbow Sorbet
• Angel Face
• Class Act
• Electron
• Jean Kenneally
• Perfecta
• Shining Hour
• Carrot Top
• Colossus
• French Lace
• Joseph’s Coat
• Picotee
• Sun Sprinkles
• Merlot
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Cultivars that will be
difficult to maintain in
a post-JB world
Cultivars bees
do not visit –
insecticide
options are
much greater
Cultivars that JB doesn’t damage in the first place
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Roses that are Heavily Damaged by JB and
Visited alot by Pollinators
• Prima Donna
• Carefree Delight
• Carefree Spirit
• Climbing New Dawn
• Day Dream
• Easy Does It
• Elle
• Eureka
• First Edition
• Fourth of July
• Glowing Peace
• Honey Perfume
• Hot Coco
• Lady Elsie May
• Moon Dance
• Morden Sunrise
• Pescali
• Rainbow Knock Out
• Starry Night
• Touch of Class
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What should we do about
the Japanese Beetle?
We should try to systematically
categorize all rose cultivars for
susceptibility to Japanese beetle
– and use by pollinating bees
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The curious phenomenon of geranium toxicity
to Japanese beetle
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Geranium Toxicity to Japanese Beetle
• Beetles become paralyzed
within a couple of hours after
feeding on flowers of zonal
geraniums – Often recover in lab, rarely outdoors
• Toxin is present in flowers,
but not leaves
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Recommendations for Japanese
Beetle Adult Control
• Physical/Cultural Controls
–Traps
–Hand Picking
• Chemical Controls
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Japanese beetle controls
Hand Picking
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Primary benefit from handpicking
Reduction in volatile
attractants produced by plant
wounding
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Handpicking may be more
effective for infestations on
foliage versus flowers
Some flowers (e.g., rose) can
produce high levels of volatile
attractants - without wounding -
and may possess attractive
colors
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Can time of day when you hand pick
have effects for JB management?
Possibly yes. It may be more
effective to handpick in the
evening than at other times of
the day for reducing numbers of
beetles on the plants.
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Japanese beetle traps are
excellent for detecting presence
of the insect in an area
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Japanese beetle traps are
minimally useful - at best - for
control of existing Japanese
beetle infestations!
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If you insist on using a Japanese
beetle trap
• Do not place them anywhere near (at least
30 feet away from) any plant on which
Japanese beetles feed
• Avoid placing them in a site where they are
likely to draw beetles from long distances
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If you insist on using a
Japanese beetle trap
• Do not place them anywhere near (at least 30 feet away from)
any plant on which Japanese beetles feed
• Avoid placing them in a site where they are likely to draw
beetles from long distances
….and preferably give the trap to
your neighbor!
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Chemical Controls Most Effective for
Control of Japanese Beetle Adults
• Most pyrethroids (e.g., cyfluthrin,
permethrin, bifenthrin)
• Carbaryl
• Acetamiprid
• Imidacloprid
• ChlorantraniliproleDo not treat
plants with
flowers in
bloom!
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Overlap of adult feeding on flowers
– and use of those flowers by
pollinators
Never apply persistent
insecticides to plants that are
in flower and attractive to
pollinators!!
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Chemical Controls Most Effective for Control
of Japanese Beetle Adults
• Most pyrethroids (e.g., cyfluthrin,
permethrin, bifenthrin)
• Carbaryl
• Imidacloprid
• Acetamiprid
–Ortho Rose and Flower Killer
• Chlorantraniliprole– Acelepryn
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Labeled for use on turfgrass
and landscape ornamentals
Environmental hazards
statements do include
warnings for aquatic
organisms.
They do not have any
pollinator warning
statements.
This is very unusual.
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Btg - Bacillus thuringiensis var. galleriae
Sold as beetleGONE! in commercial/
ag markets
Sold as beetleJUS in gardener
market
Adult
control
Weevils Scarab Beetles
Btg-susceptible Insects
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Bacillus thuringiensis (Bt)
• Derived from a widely distributed
soil bacterium
• Active ingredient a toxic protein
crystal that destroys cells of the
midgut
• Used as a stomach poison
• Several different strains – each
effective against different insects
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Several Bt strains are present, each with
specific activity
Caterpillars – kurstaki,
aizawi strains
Leaf beetles –
tenebrionis strain
Gnat, mosquito larvae –
israelensis strain
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Btg - Bacillus thuringiensis var. galleriae
Sold as beetleGONE! in commercial/
ag markets
Sold as beetleJUS in gardener
market
Adult
control
Weevils Scarab Beetles
Btg-susceptible Insects
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beetleJUS treated
Water check
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beetleJUS for adult
Japanese beetle?
Provides good reduction in feeding injury by Japanese beetle
Provides fair mortality of Japanese beetles and mortality is slow
Persistence of effects probably a few days
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Bee hazard warnings
and use restrictions?
None. You can apply this
product to plants in bloom
when bees are visiting.
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After application:
Are they dead?
(probably not)
Are they still feeding?
(probably not)
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Japanese beetle traps are
excellent for detecting
presence of the insect in an
area
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91
This is a map
of the JB
situation in the
Denver metro
area ten years
ago
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What should we do about
the Japanese Beetle?
We should conduct a systematic
survey of Japanese beetle
throughout the Front Range
corridor – a snap shot of its
status in 2019.
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Japanese Beetle Trapping Program for
2019
• Establish a network of
Japanese beetle traps
throughout the Front
Range areas of eastern
Colorado
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Japanese Beetle Trapping Program for
2019• Establish a network of Japanese beetle traps throughout the Front Range areas of eastern
Colorado
• Traps will be maintained throughout the season
(late June-Labor Day) to determine a total
number of beetles at the site during 2019• Most cooperators will be identified and coordinated by County Extension offices (e.g., Master
Gardeners)
• Some traps can be provided directly
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Japanese Beetle Trapping Program for
2019• Establish a network of Japanese beetle traps throughout the Front Range areas of eastern
Colorado
• Traps will be maintained throughout the season (late June-Labor Day) to determine a total
number of beetles at the site during 2019
• Most cooperators will be identified and
coordinated by County Extension offices (e.g.,
Master Gardeners)
• Some traps can be provided directly– [email protected]
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Do you have >a lot< of Japanese beetles in your trap??
There are
about 836
Japanese
beetles per
cup
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Should we now consider trying to introduce – for
permanent establishment - natural enemies of Japanese
beetle into Colorado?
Yes!
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What should we do about
the Japanese Beetle?
We should attempt transfers, for
permanent establishment in
Colorado, of some Japanese
beetle natural enemies present in
states to the east.
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Natural Enemies of Japanese Beetle Exist
Elsewhere in the US
• Paenibacillus popilliae (Milky spore)
– Bacterium
• Istocheta aldrichi
– Tachinid fly
• Tiphia vernalis
– Parasitoid wasp
• Ovavesicula popilliae
– Fungus (microsporidium)
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Milky Spore for Japanese Beetle?
Used to permanently establish a biological control
organism – not useful for immediate control.
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Natural enemies of Japanese Beetle that were introduced once
and now established elsewhere in the United States
• Paenibacillus popilliae (Milky spore)
– Bacterium
• Ovavesicula popilliae*–Microsporidium (fungus)
• Istocheta aldrichi*–Tachinid fly
• Tiphia vernalis* –Parasitoid wasp
* Species involved in Colorado Japanese Beetle Biological Control Program
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Natural Enemies of Japanese Beetle for Potential
Introduction into Colorado?
Ovavesicula popilliae – a fungal disease of Japanese
beetle larvae
Ovavesicula infection of Malpighian
tubules of Japanese beetle larva
Target stage – larvae in soil
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Ovavesicula popilliae infects the Malpighian tubules of Japanese beetle larvae and adults
Normal
Infected Malpighian tubules packed with spores of Ovavesicula popilliae
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The Malpighian tubules of insects filter wastes from the blood, functioning somewhat like what the kidney does in humans
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Natural Enemies of Japanese Beetle for Potential
Introduction into Colorado?
Ovavesicula popilliae – a fungal disease that
infects Japanese beetle larvae
Main observed effects from infection –
reduced winter survival (larvae), shortened
life span, reduced fecundity (adults)
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Ovavesicula popilliae: % infection of adult Japanese beetles at ten golf courses in Michigan from 2000 to 2016
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Japanese beetle trap catches at ten golf courses in southern Michigan from 1999 to 2016
Source: David Smitley, Michigan State University
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Japanese beetles collected from Michigan that were infected with Ovavesicula
popilliae were shipped to us in late July 2015. The beetles were frozen, so no
live beetles were introduced, but spores of the pathogen are still viable.
Experimental releases of Ovavesicula
popilliae were first made in 2015
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The infected (but dead and frozen)
beetles were applied in several ways
Inserted whole beetles into soil
Broadcast whole beetles on surface
Blended and applied as slurry
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Dead, frozen
Op-infected
beetles
arrive
Beetles are
blended into a
slurry
Diluted with water the slurry is
poured over sites where high
numbers of JB grubs are
present, and immediately
watered in
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2015 releases of
Ovavesicula popilliae – It
took!
Positive infections confirmed in
2017 from both Flatirons Golf
Course (Boulder) and Pueblo Zoo
release sites!!!!
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Ovavesicula popilliae as a JB Biocontrol Agent
• Colorado release sites as of today
–Pueblo (2), Boulder (1), Littleton (2), Cherry Hills (1), Denver (3)
• Characteristics needed for future release sites
–Site must have high numbers of JB grubs–Site must be at least 1 mile from any previous release site
–Someone must commit to maintaining a JB trap at the site for
the first year of the release
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Istocheta aldrichi – the “winsome fly”
Diptera: Tachinidae
The adult flies glue their eggs onto adult Japanese beetles. The larva tunnels into and kills the beetle.
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Hatched egg
Pupa of the fly larva that
developed within and
killed the Japanese beetle
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Istocheta aldrichi as a JB Biocontrol Agent
• Colorado release sites as of today
–Pueblo (1), Littleton (1), Denver (1)
• Characteristics needed for future release sites
–Site must have high numbers of JB adults
–Site must have abundant amounts of suitable
flowering plants during July–Site must be at least 1 mile from any previous release site
–Someone must commit to maintaining a JB trap at the site for
the first year of the release
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Tiphia vernalis spring tiphia
Hymenoptera: Tiphiidae
The adult wasps dig into the soil and lay their eggs on late stage Japanese beetle grubs
Photographs courtesy of David Shetlar, the Ohio State University
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Tiphia vernalis as a JB Biocontrol Agent
• Colorado release sites as of today
–Littleton (1)
• Characteristics needed for future release sites
–Site must have high numbers of JB larvae
–Site must have abundant amounts of suitable
flowering plants during May–Site must be at least 1 mile from any previous release site
–Someone must commit to maintaining a JB trap at the site for
the first year of the release
![Page 118: What should we do about the Japanese Beetle?€¦ · –Provision of water to allow tolerance and recovery from root loss . White grubs damage turfgrass by feeding on roots. Grasses](https://reader035.vdocuments.mx/reader035/viewer/2022071008/5fc68d057bf014070e0e637a/html5/thumbnails/118.jpg)
What can we hope for with the Japanese Beetle
Biological Control Program?
• One or more of the organisms will establish and
reproduce at the release site
• The organism(s) will then spread on their own over
time to cover a wide area
• As the organisms establish and increase,
populations of Japanese beetle will decrease
–These reductions will be permanent
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These projects need Green Industry Support!
• Rocky Mountain Regional Turfgrass Association
• Colorado Agricultural Experiment Station
• Colorado Nursery and Greenhouse Association
(pending support from other sources)
Thank you!!!!!