{
Nanoparticle Mediated Tumor Vascular Disruption: A Novel
Strategy in Radiation TherapySijumon Kunjachan, Alexandre Detappe, Rajiv Kumar, Thomas Ireland,
Lisa Cameron, Douglas E Biancur, Vincent Motto-Ros, Lucie Sancey, Srinivas Sridhar, G. Mike Makrigiorgos, and Ross I Berbeco.
Presented by,Mr. Syed Baseeruddin AlviRollNo. BM16RESCH11005
Subject: BionanotechnologyDepaetment: BMEIndian Institute of Technology Hyderabad Date: 15-09-
2016
Journal club discussion
It is challenging to treat deeply seated non resectable tumors by radiation therapy.
Problem Statement
• Inefficiency in passive targeting (EPR) of fully developed tumors
• Hindrance posed by dense fibrous microenvironment of solid tumors
• Off target toxicity due to radiation
A strategy to intensify tumor damage and spare the healthy tissue is desirable.
A dual-targeting strategy using vessel-targeted-radiosensitizing gold nanoparticles and
conformal-image guided radiation therapy to specifically amplify damage in the tumor
neoendothelium
Innovation
Experiment & Resultsa b c
d
(a) Conjugated AuNP (b) DLS of naked AuNP & conjugated NP (c) Absorption & fluorescence spectra of the same (650/668nm) (d) TEM image of conjugated NP (e)
Confocal imaging with RGD:AuNP (red) and FITC-Dextran (green) shows a high degree of vascular damage in targeted NP (+IR)
fe
In v
ivo
anal
ysis
g
(g) SARRP images of panc-1 tumor xenograft (r-100%, y-60%, g-10%) (h) bio distribution by ICPMS
(I-L) laser induced breakdown spectroscopy of tumor tissue post
NP administration
h
Au (p
pm) p
er g
m o
f tis
ue
This dual-targeting strategy could help maximize the therapeutic index by Increasing tumor damage Reducing the amount of radiation dose needed and therefore
limiting collateral damage to healthy tissues.
Conclusion
In v
ivo
anal
ysis
Thank you