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The Science and Magic of MushroomsMichael Schatte
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What we do• Fresh Mushrooms Available Weekly
• Oyster Mushroom Varieties
• Mini Mushroom Farm Kits
• Basic and Advanced Classes
• In-depth Mycology Courses coming soon!
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Gourmet Mushrooms
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Mycorrhizal Fungi
Wikipedia Commons Nefronus
Myco (fungus) + rrhizae (root)
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Isotope tracing Source: http://sitn.hms.harvard.edu/flash/2019/exploring-the-underground-network-of-trees-the-nervous-system-of-the-forest/
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Purpose of a Mushroom
• Reproduction - Produce and spread spores
• Food – Most have benefit of being
delicious and edible
• Communication – Complex processes to
create Psilocybin, but why?
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Fungi Lifecycle
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Mycelium• Main vegetative part of fungi
• Consists of massive network of microscopic hyphae threads
• Consumes substrate by producing enzymes
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Legal Disclaimer
Midnight Mushroom Co and myself are not advocating or endorsing the breaking of US federal laws by cultivating illegal Psilocybe mushrooms. The content of this presentation is solely for educational purposes. Only you are responsible for your own actions
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Preparation
• Gather Supplies
• Sterilization
Inoculation
• Introduce mycelium to substrate
Incubation
• Consumption of substrate
Fruiting
• Growth of mushrooms
Cultivation Cycle
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Substrate • Primary Decomposers – Wood
• Oyster mushrooms, Chanterelle, Chicken
of the Woods, Lions Mane, Psilocybe
• Secondary Decomposers – Compost
• White Button, Cremini, Portobello,
Shaggy Mane, Psilocybe
Preparation
InoculationIncubation
Fruiting
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SubstrateSupplemented Hardwood (50/50 Mix)
• 2.5 Cups - Hardwood Fuel Pellets• Oak is best, AVOID PINE!
• Available at Atwoods, Home Depot, Tractor Supply (only during winter)
• 2.5 Cups - Soybean Hull Pellets • Available at locally owned feed stores
• Handley Feed Store, Fort Worth
• 1.4 Liters - Tap Water
• Requires pressure cooker or atmospheric steam sterilizer
Preparation
InoculationIncubation
Fruiting
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SubstrateCoco Coir/Vermiculite
• 1 Brick Coco Coir• Available at Home Depot, Walmart, etc.
• 2 Qt Vermiculite• Available at Home Depot, Walmart, etc.
• 4 Qt Water
• 5 Qt Grain Spawn • Highly recommended over LC and multispore syringe
Preparation
InoculationIncubation
Fruiting
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Substrate Bagger
• Provides exact measurements automatically
• Easily create mushrooms bags quickly
• Commercial volume
• Home User: Use basic measuring cups
Preparation
InoculationIncubation
Fruiting
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Container
• Provides sterile environment inside
• Bags, Plastic Totes, etc.
• Retains moisture
• Breathable
Preparation
InoculationIncubation
Fruiting
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Sterility!
• Kill off and prevent any competing molds, bacteria and other fungi.
• 91% Alcohol FTW!!
• Substrate
• Work area
• Tools
• Yourself
Preparation
InoculationIncubation
Fruiting
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Contamination
• Trichoderma
• Cobweb Mold
• Bacteria
• Competition for nutrients
• Fungi isn’t contaminated, substrate is
Preparation
InoculationIncubation
Fruiting
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Spawn
• Multi-spore syringe• Individual spores, requires germination
• Liquid Culture• Living mycelium in nutrient liquid
• Grain Spawn • Mycelium attached to nutritious grains
Preparation
InoculationIncubation
Fruiting
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Preparation
InoculationIncubation
Fruiting
Sterilization
• Substrate must be sterilized (or super pasteurized)
• Atmospheric Steam Sterilizer
• Home users: Highly recommend pressure cooker
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Laminar Flow Hood
• Commercial or dedicated hobbyist
• Creates clean room environment in airflow
• HEPA filters out 99.997 of contaminants
• Used to inoculate bags/plastic containers
Preparation
InoculationIncubation
Fruiting
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Still Air Box• Great for Home Users
• Provides controlled sterile environment.
• Lab work at home
• Cheap!
Preparation
InoculationIncubation
Fruiting
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Inoculation
• Introduction of mycelium into sterile substrate
• Use SAB or Flow Hood for this!!
• 3 Methods• Multi spore syringe (slow)
• Liquid Culture
• Grain Spawn
Preparation
InoculationIncubation
Fruiting
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Inoculation
• Bag is sealed to avoid contamination
• Mixing of substrate with spawn
• Home Users: Can use
sterile tools or gloves to mix substrate
Preparation
InoculationIncubation
Fruiting
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Incubation• 2-3 weeks for most species
• Be patient!
• No need for warming, room temp is fine
• Don’t open it or you risk contamination
Preparation
InoculationIncubation
Fruiting
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Incubation
• Contamination is more susceptible during the early stage
• Mycelium produces its own defenses to contamination
Preparation
InoculationIncubation
Fruiting
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Fruiting
• Now we’re growing mushrooms!
• Main triggers for most species• Fresh Air
• Humidity
Preparation
InoculationIncubation
Fruiting
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Fruiting Chamber
• Fresh Air Exchange • Mushrooms make CO2 and need oxygen
• Exhaust fan or venting
• Humidity • Ultrasonic Disc Humidifier “Cool Mist”
• Perlite – rocky material that retains water
Preparation
InoculationIncubation
Fruiting
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Fruiting Chamber
• Shot Gun Fruiting Chamber
• 64 Qt Tote
• 3-4 in. moistened Perlite
• Simple, low maintenance
• Climate Controlled Mini Greenhouse
• Requires humidifier
• Exhaust Fan
• Must balance humidity with fresh air
Preparation
InoculationIncubation
Fruiting
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Great Resources
• Midnight Mushroom Growers (FB Group)
• Mushroom Growing (FB Group)
• Shroomery.org (Tons of Tek’s)
• r/Shrooms (Reddit)
• r/Mycology (Reddit)
• r/SporeTraders (Reddit)
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Michael Schatte
Facebook.com/MidnightMushroomCo
www.MidnightMushroomCo.com/Conference
Get these slides and other great
info!