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The 2007 UK FMD outbreak:The 2007 UK FMD outbreak:
field investigation perspectivefield investigation perspective
Nick Juleff, Institute for Animal Health
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UK 2007 FMD EpidemicUK 2007 FMD Epidemic� Virus escape from Pirbright site (IAH
and Merial vaccine plant)
� 8 infected premises, most consisting of multiple holdings
� Mainly extensive beef production
� Most farms in semi-urban areas
� Part-time/hobby farmers
� 1578 animals on IPs culled
� 278 animals infected
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IP1: 3 holdingsIP1: 3 holdings� 29th July: farmer notices an animal “off colour”
� 2nd August: several cattle lame & drooling. PVP tells farmer to contact Defra directly.
� 3rd August: Defra vet examines & takes samples; +ve for FMDV
� 4th August: cattle at all 3 holdings killed; virus identified as O1 BFS 1860 (used at Pirbright site)
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IP1: main holdingIP1: main holding•38 cattle, all infected
•Lesion ages: 3 to 10 days old
•Beef store cattle grazing on open pasture 4km from Pirbright site
•No handling facilities: Defra brought in gates & straw bales to make corral
•Shot with rifles then pithed
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IP1: two other holdingsIP1: two other holdings
� 4 cattle at home farm: no FMDV
� 22 cattle on open pasture: no FMD lesions; one animal PCR +ve (viraemic)
� Only link between premises: farmer
� First time preclinically viraemic animals detected using PCR in an outbreak
� Logistical problems again: straw bales, rifles
� Carcases transported by sealed lorry to incinerator 70 miles away
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IP2: three holdingsIP2: three holdings� 49 cattle 1km from IP1; 58 at home farm; 12 on third
holding
� 6th August: farmer notifies Defra, samples taken from holding with 49 cattle: +ve
� 25 killed that night, the rest on next day
� Cattle examined & bled post-mortem
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IP2IP2
� 49 cattle: 44 had lesions, 2-7 days old
- Logistical probs: no handling facilities, no water, rifles, journalists
� 58 cattle on 2nd farm: no FMD lesions BUT
- 15 of 58 viraemic by PCR/VI
� 12 cattle at 3rd holding: no FMD
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Phase 1: AugustPhase 1: August
� 3 Aug: IP1 (3 locations)
� 6 Aug: IP2 (3 locations)
� 3 contact herds culled
� 24 Aug: PZs lifted
� 8 Sep: SZ lifted
� Origin: contamination
from Pirbright site
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Likely times of lab escape of virus from Likely times of lab escape of virus from
Pirbright site in 2007Pirbright site in 2007
� 20th July: flooding
� 23rd July: best airborne spread day
� 22nd July: 1st centrifuge waste discharged
� 25th July: 2nd centrifuge waste discharged
� 20th July: 4 lorries via Westwood Lane
� 25th July: 2 lorries via Westwood Lane
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Effect of lesion age and
incubation period on time of infection
NB Could be further lag between escape of virus and exposure of cattle
Flo
odin
g +
lorr
y m
oves
Air
born
e sp
read
day
1st
Mer
ial
slurr
y r
elea
se
2nd M
eria
l sl
urr
y r
elea
se
And l
orr
y m
oves
July August
9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 1 2 3 4
-15 -14 -13 -12 -11 -10 -9 -8 -7 -6 -5 -4 -3 -2 -1 1 2 3 4 5 6 7 8 9 10 11 12
July August
11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 1 2 3 4
-15 -14 -13 -12 -11 -10 -9 -8 -7 -6 -5 -4 -3 -2 -1 1 2 3 4 5 6 7 8 9 10
July August
13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 1 2 3 4
-15 -14 -13 -12 -11 -10 -9 -8 -7 -6 -5 -4 -3 -2 -1 1 2 3 4 5 6 7 8
10 d
ay o
ld +
/-2 d
ays
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DifficultiesDifficulties
� Assuming lesion of 12 days and longish
incubation then likely release times too late
� Flooding period fits best as time of release most
consistent with lesions and incubation
� But flooding and first lorry movements precede
likely virus release through drains
� Second period of lorry movements more likely
associated with viral contamination, but too late
to have infected farm
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EGHAM
WINDSOR
WOKING
GUILDFORDIP1a
IP2a
IP2b
IP5
IP7
IP6
IP3c
IP4
IP3b
EGHAM
WINDSOR
WOKING
GUILDFORDIP1b
IP2b
IP2c
IP5
IP7
IP6
IP3c
IP4b
IP3b
PIRBRIGHT SITE
5 km
EGHAM
WINDSOR
WOKING
GUILDFORDIP1a
IP2a
IP2b
IP5
IP7
IP6b
IP3c
IP4
IP3b
EGHAM
WINDSOR
WOKING
GUILDFORDIP1b
IP2b
IP2c
IP5
IP7IP3c
IP4b
IP3b
PIRBRIGHT SITE
5 kmIP8
EGHAM
WINDSOR
WOKING
GUILDFORDIP1a
IP2a
IP2b
IP5
IP7
IP6
IP3c
IP4
IP3b
EGHAM
WINDSOR
WOKING
GUILDFORDIP1b
IP2b
IP2c
IP5
IP7
IP6
IP3c
IP4b
IP3b
PIRBRIGHT SITE
5 km
EGHAM
WINDSOR
WOKING
GUILDFORDIP1a
IP2a
IP2b
IP5
IP7
IP6b
IP3c
IP4
IP3b
EGHAM
WINDSOR
WOKING
GUILDFORDIP1b
IP2b
IP2c
IP5
IP7IP3c
IP4b
IP3b
PIRBRIGHT SITE
5 kmIP8
Phase 2: Phase 2:
SeptemberSeptember
IP3IP3
� Confirmed 12th Sept� 8 locations: 2 FMD+ve� 36/47 cattle had FMD lesions, 1-5 days old� 9/29 cattle had lesions at 2nd holding, 1-5
days old� Other holdings all FMD negative� Culling completed 16 Sep (4 days later)� Logistical probs on out-farms:No handling facilities, escapees, police
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IP4IP4
� Separated from IP3 by narrow stream
� Diagnosed Sept 13th (farmer report)
� 54/54 beef cattle had FMD lesions, 5-10 days
� 800 pigs kept 500m away: no FMD
� Farmer had been on holidays for 10 days – unclear who checked cattle
� Excellent handling facilities –fast cull
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IP5: the missing linkIP5: the missing link
� After IP3 & 4, 3km PZ implemented
� All sheep bled, cattle & pigs “clinically inspected” – often just observed over fence
� 17th Sep: IP5 sheep tested seropositive
� IP5: 16 sheep, 22 cattle, 2 pigs
� Farmer retired & unwell; animals were pets
� FMD lesions ~ 3 weeks old (large error margin)
� Virus detected in probangs – 10/16 sheep carriers
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Crown Estates
Cook
Berryman
Regan
Whitty
Salmon
Wallace
Bovington
IPA ongoing
IPA culled
Raynen
Robertson
Harvey
IPA ongoing
sheepWiggins
Intensive patrol areas:Intensive patrol areas:
••Animals examined every 2 days (in theory)Animals examined every 2 days (in theory)
••Blood samples: real time PCR to detect Blood samples: real time PCR to detect
viraemic preclinicals viraemic preclinicals
••Small no. of farmsSmall no. of farms
••Some premises: clinical examination very Some premises: clinical examination very
difficult (no facilities)difficult (no facilities)
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IP6� 21st Sept, Defra inspection� 34 cattle, 2 locations� 2/32 cattle with 3-4 day
lesions� Another 5 viraemic
IP7� 24th Sept, Defra inspection� 16 Dexter cattle, wild &
unhandled� Hobby farmer: retired
architect� 14 with 1-5 day lesions� All FMDV +ve� Use of LFD on farm� Logistical probs: xylazine
darts, rifles; urban area
IP8�29th Sept, Defra inspection�54 beef cattle at infected site �1 with lesions, 2 days; 15 viraemic�134 cattle, 16 sheep�4 locations, 134 cattle, 16 sheep�Other sites FMDV -ve
XX
ALDERSHOT
HEATHROWWINDSOR
XXXX
WOKING
XX
M4
XXGUILDFORD
EGHAM
XX
XXXX
M3
M25
GODALMING
1c
1b
2b
2c
1a
2a
Pirbright site
8
7
6b3b
3c
5
4b 3a
3d
3e
3f
3g
3h
XX
ALDERSHOT
HEATHROWWINDSOR
XXXX
WOKING
XX
M4
XXGUILDFORD
EGHAM
XX
XXXX
M3
M25
GODALMING
1c
1b
2b
2c
1a
2a
Pirbright site
8
7
6b3b
3c
5
4b 3a
3d
3e
3f
3g
3h
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Shared IAH / Merial FacilityShared IAH / Merial Facility
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Source of virus: Pirbright siteSource of virus: Pirbright site� Virus: O1 BFS1860 (1967 UK outbreak)
� 3 strains used on Pirbright site:
IAH1 (v. small quantities, Institute for Animal Health research)
IAH2 (v. small quantities, Institute for Animal Health research)
MAH (6,000 litre batches for vaccine production, Merial Animal Health)
� Molecular epidemiology reveals transmission pathways
(Cottam et al, 2008, PLoS Pathogens 4(4): e1000050 )
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Genealogy and infection profilesGenealogy and infection profiles
Cottam et al, 2008, PLoS Pathogens
Incubation period (<14 days)
Most likely date of infection (2-5 days before clinical disease)
Lesion age estimation
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� Merial vaccine waste inactivation method not validated for large volumes
� Permitted by Defra (UK Govt) – MAH did not breach their licence
� Dispute over responsibility for pipes
� Unresolved questions:
How did virus get from Pirbright to IP1?
How credible is the pipe/soil/lorry theory?
How did it get from IP1/2 to IP5?
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Lessons from the fieldLessons from the field� Epidemiological benefits of lesion ageing, extensive
sampling, sequencing virus isolates in real time
– 2nd phase of outbreaks (IP3 – IP8) shares all the unique changes common to 1st phase
– Therefore outbreaks are linked and not due to independent sources
– IP5 (farm with FMD serology positive cattle and sheep) bridges gap between two phases of the outbreak
� Diagnosis of preclinical viraemic animals using real-time PCR
� Hobby/part-time farmers with inadequate handling facilities: implications for neighbouring farmers?
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AcknowledgmentsAcknowledgments
David PatonDonald King Eoin Ryan
Bryan CharlestonColleagues at the IAH