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Synthetic Biology
and iGEM
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International
Genetically Engineered
Machine (iGEM)
Competition
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iGEM Jamboree 2015
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Synthetic biology: what
is it?
Cover of Man
Modified, David
Fishlock
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“The Lego of Life”
http://souterre.com/biobricks/
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Synthetic biology:
how do we do it?
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Synthetic biology: why is
it important?
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Synthetic biology:
why is it important?
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Synthetic biology:
why is it important?
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Transcription and
Translation
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Our Project:
Paper-Based Cell-Free Thallium
Sensor
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Paper-Based Sensor
BBC, “Prototype paper test can detect Ebola strains”
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Cell-Free
picky
time
consuming
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Thallium
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Our Project:
Paper-Based Cell-Free Thallium
Sensor
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Genetic Circuits
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Genetic Circuit
Diagram
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Toehold Switch
Mechanism of the Toehold Switch, Harvard University,
2014
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Cell-Free, Paper-Based
Sensor
Paper-Based Synthetic Gene Networks, Pardee et
al., 2014
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Cloning
Step 1: Cut DNA
Step 2: Gel
Electrophoresis
Step 3: Ligate DNA
Step 4:
Transformation
vector
insert
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PCR• Polymerase Chain Reaction