Download - Syllabus of MSc (Micro) OU
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DEPARTMENT OF MICROBIOLOGY, OSMANIA UNIVERSITY
M.Sc. (Previous MICROBIOLOGY (Se!es"er S#s"e!
S#$$%&us, Sc'e!e o I)s"ruc"io) %)* E+%!i)%"io)
Se!es"er I(15 Weeks)
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Paper Sub.Code Subject/Paper Title Instruction Ea! "a
#o. $rs/Wk Ti!e $rs "arks
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T$E%&'
I " 11 *eneral "icrobiolo+, 1
II " 1 0irolo+, 1
III " 1 io!etr, and Co!puters 1
I0 " 1 ioce!istr, 1
P&2CTIC23S
I " 151 *eneral "icrobiolo+, a -
II " 15 0irolo+, b -
III " 15 io!etr, and Co!puters c -I0 " 15 ioce!istr, d -
" 144 Se!inar - -
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Total . 6
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Se!es"er II(15 Weeks)
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Paper Sub.Code Subject/Paper Title Instruction Ea! "a
#o. $rs/Wk Ti!e $rs "arks
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T$E%&'
I " 1 "icrobial P,siolo+, 1
II " I!!unolo+, 1
III " 2nti!icrobial 2+ents and Ce!oterap, 1
I0 " En7,!olo+, and ioce!ical Tecni8ues 1
P&2CTIC23SI " 51 "icrobial P,siolo+, 9(a) 1
II " 5 I!!unolo+, 9(b) 1
III " 5 2nti!icrobial 2+ents and Ce!oterap, 9(c) 1
I0 " 5 En7,!olo+, and ioce!ical Tecni8ues 9(d) 1
" 44 Se!inar - -
------------------------------------------- Total . 6
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#ote: (a) Te ea!ination includes bot " 151 and " 51 s,llabi
(b) Te ea!ination includes bot " 15 and " 5 s,llabi
(c) Te ea!ination includes bot " 15 and " 5 s,llabi
(d) Te ea!ination includes bot " 15 and " 5 s,llabi
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M.Sc. (Previous I Se!es"er
P%er I MB -- Ge)er%$ Micro&io$o/# (0 U)i"s + -1 2rs 3 4 2rs "e%c'i)/
U)i" I
$istor, o; "icrobiolo+,. "icroscop,. Structure o; !icrobial cells:Spontaneous +eneration and +er! teor, o; disease< Contributions o; 2nton, =an
3eeu>enock< 3ouis Pasteur< &obert ?oc< Ed>ard @enner< Wino+radsk,< eijerinkorkin+ o; bri+t ;ield !icroscope< ;luorescent !icroscope< pase contrast
!icroscope< electron !icroscope. 2pplication and i!portance o; abo=e !icroscopes.
"easure!ent o; !icroscopic objects. Prokar,otic cell< Eukar,otic cell< %r+ani7ationand ;unction o; cellular or+anells
Cell >all o; bacteria< cell !e!branes< ;la+ella< pili< capsule structure< ce!ical structure
o; peptido+l,can< protoplasts< speroplasts< !icroso!es and riboso!al s< #uclear!aterial/nucleus.
U)i" II
"etods o; sterili7ation: P,sical !etods Br, eat< !oist eat< radiation !etods o; !edical !icrobiolo+, b, @a>it7< !elnick and 2delber+
acterial and ",cotic in;ections o; !an. Ed. Bubos and $irst 3ipincott
Principles o; "icrobiolo+, and I!!unolo+, b, Ba=is< Bulbecco < Eison< *insber+ andWood.
"icrobiolo+, b, Pelc7ar ".@.< &ied< &B and Can< ECS.
Structure and &eproduction o; 2l+ae< 0ol. I F II b, Aritsc< A.E.
Introduction to 2l+ae b, "orris< I.Products and Properties o; 2l+ae b, Gi7ac.
Ares >ater al+ae o; te Hnited States b, S!it< *".
Introductor, ",colo+,< b, 2leopolus< [email protected] in Aun+i b, In+old< CT
"icrobial P,siolo+, b, "oater !osaic =irus
U)i" II
Structure and replication o; bacteriopa+es: 3,tic ds linear B#2 =iruses (T< T)
3,so+enic ds linear B#2 =irus (3a!bda) ss Circular B#2 =irus (J K 1) "alespeci;ic ;ila!entous ss =irus (A1 and "1)
U)i" III&eco!bination in pa+e< !ultiplicit, reacti=ation and penot,pic !iin+.
Structure and replication o; ani!al =iruses (2deno=irus (e+. T,pe )
U)i" IV
Structure and r eplication o; ",o=iruses (e+. In;luen7a) Po =irus (e+. 0accinia)
$epatitis =irus.
E;;ect o; ani!al =irus in;ection on ost cell 0iral inter;erence and inter;eron Tu!or
=iruses (B#2 and )
I Se!es"er P%er II MB -16 Viro$o/# (Pr%c"ic%$s
Isolation o; pa+e ;ro! soil.Culti=ation and preser=ation o; pa+es
Luantitation o; pa+es
*ro>t pa+es o; pa+e and burst si7e
Isolation o; pla8ue t,pe and ost ran+e !utantsPa+e induction
3,so+en,-Transduction
Propa+ation o; ani!al =iruses in e++ allantoic< a!niotic and C2"Propa+ation o; =iruses in tissue culture
Pla8ue titration and neutrali7ation
Luantitation o; =irus b, $2< Pock titration< pla8ue titrationIdenti;ication o; =irus $2I
Be!onstration o; c,topatolo+ical can+es
S,!pto!atic obser=ations o; plant =iral in;ections
Cloroplast a++lutination o; plant =iruses
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Reco!!e)*e* Boo5s
*eneral 0irolo+, b, 3uria and Barnel
0irolo+, and I!!unolo+, b, @okliTet book o; 0irolo+, b, &odes and 0an &o,en
Plant 0irolo+, b, S!it
*enetics o; bacteria and teir =iruses b, W. $a,es"olecular iolo+, o; te +ene b, Watson< &oberts< Stait7 and Weiner
2 laborat+or, +uide in =irolo+, b, Cjarles $. 3unnin+a!
asic lab procedures in dia+nostic =irolo+, b, "art, Cristensen
M.Sc. (Previous I Se!es"er
P%er III MB -7 Bio!e"r# %)* Co!u"ers
(0 u)i"s + -1 'rs 3 4 'rs "e%c'i)/
U)i" I
Introduction to statistics and bio!etr,
Population< sa!ples and sa!plin+ procedures< =ariables< =ariations and ;re8uenc,
distributions< !easures o; a=era+es and dispersion< ele!ent o; probabilit,< +ausian ornor!al distribution< bino!ial distribution< poisson distribution< MtD distribution< MAD
distribution and Ci-s8uare distribution< correlation and linear re+ression >it a special
re;erence to applications in biolo+,
U)i" II
Tests o; in;erence and concepts
S!all sa!ple tests and lar+e sa!ple tests: #or!al cur=e test< MtD test< MAD test< 2#%02at is $ard>are and so;t>are< brie;
introduction on input output de=ices (Bisks< Printers< CB-&%"S and oter stora+e
!edia etc.)Introduction to disk operatin+ s,ste!s (B%S): Sa!ple co!!ands< BI&-CB-&B-BE3-
C%P'-"%0E-&E#-T'PE-EBIT (Editor) CE-B2TE and TI"E.
Introduction to Windo>s 45: Icons< Besktop< Ailes and Aolders.Si!ple operations like creation< deletion< !o=in+< cop,in+ o; Ailes or ;olders usin+
>indo>s eplorer. Searcin+ ;iles and ;olders. Creatin+ sortcuts and oter si!ple
operations.
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U)i" IV
Word Processin+: %penin+< creatin+ and sa=in+ docu!ents T,pin+< na=i+atin+ andselectin+ editin+ and sortin+-ceckin+ spellin+ and +ra!!ar ;or!attin+ can+in+
appearance o; ,our pa+e i!portin+ +rapics >orkin+ >it tables >orkin+ >it land
docu!ents Printin+Electronic Spread Seet: Creatin+< openin+ and sa=in+ ;iles >orkin+ >it >orkseets
and >ork books enterin+ data and selectin+ cells editin+ >orkseets printin+orkin+ >it carts Su!!ari7e data in lists and tables 2nal,7in+data >it Pi=ot tables 2nal,7in+ sa!ple statistical data 0alidatin+ cells.
I Se!es"er P%er III MB -17 Bio!e"r# %)* Co!u"ers (Pr%c"ic%$s
Aor!ation o; ;re8uenc, distribution< +rapical presentation and calculation o; descripti=e
!easures
Aittin+ o; distribution ino!ial< Poisson and #or!al distributionCi-s8uare test< +oodness o; ;it< contin+enc, and etero+eneit,
S!all and lar+e sa!ple tests< sa!ple !eans and propertiesProble!s on correlation and re+ression
2#%02 %ne >a, and t>o >a, classi;ications
Creatin+ docu!ents usin+ >ord processorEercisin+ te ;eatures o; >ord processor
Creatin+ spread seets
Hsa+e o; spread seet to biolo+ical applications
Reco!!e)*e* Boo5s
Introduction to te teor, o; statistics b, 2leander< " "ood and Aranklin
Aunda!entsls o; io!etr, b, 3.#.ala!
Statistical !etods b, Snedecor and CocranIntroduction to co!puter and its application b, Cae C.Cien
asic Pro+ra!!in+ lan+ua+e b, ajara!an
iostatistics 2 !anual o; statistical !etods ;or use in $ealt< #utrition and
2ntropolo+, b, ?. 0is=es>ar &ao
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M.Sc. (Previous I Se!es"er
P%er IV MB -0 Bioc'e!is"r# (0 u)i"s + -1 'rs 3 4 'rs "e%c'i)/
U)i" I
p$ and its biolo+ical rele=ance
Beter!ination o; p$< preparation o; bu;;ersConcept o; entrop,< ;ree-ener+,< ;ree ener+, can+es< i+ ener+, co!pounds.
E8uilibriu! constants< &edo potentials< iolo+ical redo s,ste!s
U)i" II
iolo+ical oidation< iolo+ical redo carriers< biolo+ical !e!branes< electron
transport< oidati=e pospor,lation and !ecanis!. acterial potos,ntesist
!easure!ent.
U)i" IV
#ature and properties o; spores: acterial endospore structure< peno!enon o;
sporulation< bioce!istr, and +enetics o; sporulation. Induction o; sporulation
peno!enon. *er!ination o; sporesTe concept o; disease: In;ectious disease< pato+enic !icrobes< properties o;
pato+enic !icrobes< In;ection< pato+enesis and =irulence. 0irulence ;actore
Capsular !aterials< bacterial toins eotoins< tooids< endotoins< enterotoins.P,siolo+, o; toin production. Etracellular en7,!es o; pato+enic bacteria.
2pplication o; toins and toic en7,!es< laborator, testin+ !etods ;or bacterial
=irulence properties
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II Se!es"er P%er I MB 61- Micro&i%$ P'#sio$o/# (Pr%c"ic%$s
Preparation o; !icrobiolo+ical !edia. 2utotropic !edia< !ini!al !edia< basic !ediait7< !elnick and 2delber+
acterial and ",cotic in;ections o; !an. Ed. Bubos and $irst 3ipincott
Principles o; "icrobiolo+, and I!!unolo+, b, Ba=is< Bulbecco < Eison< *insber+ and
Wood.Tet book o; "icrobiolo+, b, 2nantanara,anan
"icrobiolo+, b, Pelc7ar ".@.< &ied< &B and Can< ECS.
"icrobial P,siolo+, b, "oatt b, "andelstu!< "c Luillon and Ba>es
acterial "etabolis! b, B>ellel,Potos,ntesis b, Be>lin and arker
3aborator, Eperi!ents in "icrobiolo+, b, *opal &edd, et al
"icrobes in 2ction b, Seole, $W and 0an-Be!ark< P@
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M.Sc. (Previous II Se!es"er
P%er II MB 66 I!!u)o$o/# (0 u)i"s + -1 'rs 3 4 'rs "e%c'i)/
U)i" I
rie; istor, o; i!!unolo+,< anti+ens (t,pes o; anti+ens anti+en speci;icit,a,s.
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II Se!es"er P%er II MB 616 I!!u)o$o/# (Pr%c"ic%$s
2++lutination reactions Widal< 0B&3< $2< lood +roup/t,pin+Precipitation test: &in+ interpase< sin+le radial di;;usion< %uecterlon,
Testin+ ;or dru+ sensiti=it,
Beter!ination o; "IC =alues (tube dilution !etod)Beter!ination o; penol coe;;icient (&eidel Walker coe;;icient< &WC)
Stud, ;or anti!icrobial spectru! o; anti!icrobials
Beter!ination o; cidal and static acti=it,Screenin+ ;or antibiotic producin+ !icrobes
Ce!ical assa, !etods ;or anti!icrobial dru+s
"icrobiolo+ical assa,s o; anti!icrobial dru+s
Reco!!e)*e* &oo5s
Bisin;ection< sterili7ation and preser=ation Ed. lock< SS.
"icrobial conta!aination control ;acilities Ed. &unkle< &S and Pilips< *Principles and !etods o; sterili7ation in ealt science b, Perkins @on< @.
Inibition and destruction o; te !icrobial cell Ed. $u+o
ioce!istr, o; anti!icrobial action b, Aranklin< T@ and Sno>< 3.Te "olecular basis o; antibiotic action b, *ale et al
2ntibiotics and ce!oterap, b, *errod et al
Par!aceutical !icrobiolo+o, b, $u+o &ussell
"icrobiolo+ical assa,s b, $e>itt2nti=iral dru+s b, S.?ar+er
ur+ersD !edicinal ce!istr, (all =olu!es) Ed. "an;ield E. World.
2ntibiotic interactions b, Willia!sTe control o; antibiotic resistant bacteria b, $arris and $arris
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M.Sc. (Previous II Se!es"er P%er IV MB 60 E)8#!o$o/# %)* Bioc'e!ic%$
Tec')i9ues (0 u)i"s + -1 'rs 3 4 'rs "e%c'i)/
U)i" I
En7,!es no!enclature< classi;ication !etods ;or deter!ination o; en7,!e acti=it,.
Isolation and puri;ication o; en7,!es. En7,!e kinetics: E;;ect o; p$< substrateconcentration < te!perature and inibitors. Isoen7,!es. Co!petiti=e and non-
co!petiti=e inibition
U)i" II
"ecanis! o; en7,!e action 2ction o; &ibonuclease< c,!otr,psin< and tr,psin.Coen7,!e catal,sis. "ecanis! o; P2KP% and tia!ine p,ropospate en7,!es
Control o; en7,!es. &e+ulation o; en7,!e acti=it,: allosteric en7,!es and ;eed back
!ecanis!s. "etabolic co!parta!entali7ation in relation to en7,!e< en7,!es and
secondar, !etabolites
U)i" III
%ptical !etods: Ele!entar, treat!ent o; bioce!ical !etods< colouri!etr, and
spectropoto!etr,< ;luro!etr,< optical rotation< ;luorescence
Seperation !etods: Cro!ato+rapic tecni8ues paper< tin la,er< ion ecan+e< +el;iltration and a;;init, cro!ato+rap,. Bi;;usion< dial,sis< cell disruption !etodsei+t o; B#2< resol=ed on a+arose +el electroporesis
Beter!ination o; !olecular >ei+t o; protein b, P2*E
Induction o; !utations b, p,sical/ce!ical !uta+ens< screenin+ and isolation o;!utants< &eplica platin+ tecni8ue
Trans;or!ation in bacteria
Conju+ation in bacteria
Co!ple!entation tests in bacteriaProtoplast preparation and re+eneration
Reco!!e)*e* &oo5s
"olecular biolo+, b, Ba=id and Arei;elder
"icrobial +enetics b, Ba=id and Arei;elder*enetics o; bacteria and teir =iruses b, Willia! $a,es
"olecular biolo+, o; tee +ene b, Watson et al
Te ioce!istr, o; nucleic acids b, Ba=idson @#"olecular biotecnolo+, b, Pri!erose
"olecular iotecnolo+, b, ernard &. *lick and @ack @ Pasternak
B#2 "icroarra,s Ed. ". Scena
M.Sc. (Fi)%$ III Se!es"er Micro&io$o/#
P%er II MB 76 I)*us"ri%$ Micro&io$o/# (0 u)i"s + -1 'rs 3 4 'rs "e%c'i)/
U)i" I
Introduction to industrial !icrobiolo+,. Be;inition< scope< istor,< !icroor+anis!s and data !inin+ o;
se8uence data bases. B#2 se8uencin+ !etodolo+, and so;t >are< se8uence
co!parison and ali+n!ent. Concept o; par!aco+eno!ics and its applicationIP& and patentin+ Concept and o=er=ies
P%er I MB 01- Mo$ecu$%r Bio"ec')o$o/# (Pr%c"ic%$s
&estriction !appin+PC& tecni8ue de!onstration
Plas!id detection and Isolation o; Plas!id B#2
Preparation o; co!petent cells and trans;or!ation
*ene clonin+ in bacteria de!onstration
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Soutern trans;er de!onstration
Be!onstration o; &A3P/2A3P
Induction o; en7,!es 3ac operonTransposon !uta+enesis
Bial,sis and separation o; proteins b, colu!n cro!ato+rap,
Reco!!e)*e* Boo5s
"olecular biolo+, b, Ba=id and Arei;elder
"icrobial +enetics b, Ba=id and Arei;elder
*enetics o; bacteria and teir =iruses b, Willia! $a,es
"olecular biolo+, o; tee +ene b, Watson et alTe ioce!istr, o; nucleic acids b, Ba=idson @#
"olecular biotecnolo+, b, Pri!erose
"olecular iotecnolo+, b, ernard &. *lick and @ack @ Pasternak
B#2 "icroarra,s Ed. ". Scena
M.Sc. (Fi)%$ IV Se!es"er Micro&io$o/#
P%er II MB 06 Micro&i%$ Bio"ec')o$o/# (0 u)i"s + -1 'rs 3 4 'rs "e%c'i)/
U)i" I
Aer!entati=e production o; insustrial alcool< uses< ra>!aterials< !icroor+anis!sort< ;er!entation and reco=er,
Aer!entati=e production o; beer "ediu! co!ponents< !alt< !alt a djuncts< opsater. Preparation o; >ort< !asin+< >ort boilin+< !icroor+anis!< inoculu! preparationine !akin+ Aruit selection< pickin+< crusin+< sulpite additionater - $epatitis ($20)< &!k< Polio !,elitis
Betailed stud, o; =iruses trans!itted b, Goonosis &abies< @apanese encepalitis
U)i" III
Betailed stud, o; contact and seuall, trans!itted =iral diseases S!all po< $erpes
($erpes si!ple =irus)< $epatitis =iruses and teir diseases
2c8uired i!!unode;icienc, s,ndro!e (2IBS)
U)i" IV
"alaria< 2!oebiasis< $el!itic in;ections (&ound >or!s< $ookd >or!s) o; !edical !icrobiolo+, b, @a>it7 et al
"edical laborator, "anual ;or tropical countries 0ol I F II b, "onica Ceesbrou+
Tet ook o; "icrobiolo+, b, 2nantanara,anan and @a,ara! Paniker0iral and &icketsial in;ections o; "an b, $ors;all and @a!
Tet book o; 0irolo+, b, &odes and 0an &o,an
0irolo+ical Procedures b, "itcal askin+
0irolo+o, b, Wilson and Tople,