Stable Isotope and Metabolomics Core Facility
AECOM Diabetes CenterThe Einstein Stable Isotope & Metabolomics Core is within the NIH funded Diabetes Research Center at Einstein and anchors the CMCR Metabolomics Core. The Core uses stable isotope flux and metabolite profiling to help formulate and test hypotheses about the metabolic consequences of various changes in gene expression and protein function, in order to guide further integrative systems biology analyses of the underlying mechanisms in diabetes, insulin resistance, obesity, and diabetic complications. The Core objectives includes assessments of plasma, urine and tissue metabolite profiles for diagnosis/characterization of physiological and pathophysiological states. These include key metabolites in the glycolytic, gluconeogenic, pentose phosphate, and tricarboxylic cycle pathways, fatty acid oxidation and complex lipids, such as ceramides, diacylgylcerols, triacylglycerols (TAGs), and phospholipids. In the CMCR framework, the Core will use these methodologies to explore the specific effects that radiation has on the metabolic network of each organ, and to explore the metabolic consequences of protein modification, by radiation, on the function of the metabolic network.
CMCR-AECOM
Stable Isotope & Metabolomics CoreLocation: Price 368
Director: Irwin Kurland,
MD PhDSandhya Narasimhan, PhD
Lab Members:
Albert Einstein College of MedicineOF YESHIVA UNIVERSITY
BhavapriyaVaitheesvaran, PhD
Kirsten Hartil, PhD
Hui-Min Zhang PhD
Metabolite Profiling and Flux Analyses
LC/MS Specialist
Seahorse Extracellular Flux Analyses
Mitochondrial PhysiologyAnimal Research Coordinator
XF Applications in Cellular Bioenergetic AssaysXF Applications in Cellular Bioenergetic Assays
MitochondrialFunction
In vitro ToxicityCancer
Fatty Acid Oxidation& Glycolysis
Cell quality
Neurodegenerativediseases
Translation to Clinical Applications
Cardiovascularresearch
Obesity/diabetes
XF96 ExtracellularXF96 Extracellular Flux AnalyzerFlux Analyzer
LC/MS AnalysisLC/MS Analysis
Simultaneous analysis of multiple metabolites
Waters AquityHigh resolution Chromatography
Identification of unknown species by structure elucidation
Multivariate Statistical Analyses
LTQ-Orbitrap
Waters Xevo Tandem MS
Synapt G2 Linear Hybrid quadrapole Time of Flight MS with MALDI
Synapt G2 itself Synapt G2 with MALDI front end
GC/MS Analysis of MetabolitesGC/MS Analysis of Metabolites
Lipid profiling: Fatty acids DAGs, TAGsCholesterol
Metabolites from theglycolytic pathway, TCA cycle, pentose cycle and amino acids
Stable isotope assessments of dynamic glucose disposal (SipGTT), lipogenesis ([2H]2O or U-13C]lactate), glucose production ([U-13C]glucose), glycerol production/lipolysis ([2-13C]glycerol), Cori cycle ([U-13C]lactate)
Waters Premier GC-Time of Flight-MS