Download - Selected Case Studies
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Selected Case Studies
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MIT 2006: Engineering bacteria to smell good
BSMTwintergreen
C. breweri S. cerevisia
e
ATF1banana
Slides borrowed from the 2006 MIT Team
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Engineering the scent of bacteria
What is the desired output?
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Components of engineered cell
What is needed to create the desired output?
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Components of engineered cell
What is needed to create the desired output?
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Creating devices with BioBricks
Regulatory Part/Promoter
Protein Coding Part
TerminatorRibosome Binding Site
Protein
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Components of scent-producing devices
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Components of scent-producing devices
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Components of upstream devices
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How do you regulate when the scents are expressed?
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Goal: Sense the growth state of culture
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Regulating the timing of expression
osmY: active in stationary phase & under high osmotic pressure conditions
osmY
osmY + inverter
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Regulating the timing of expression
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Design principles
• What is the desired output? What is needed to create this output?
• What do you want to sense?
• Use of constitutive promoters, inducible promoters, and inverters
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Berkeley 2006Bacterial Networks
Need: To transfer DNA messages from one bacterial cell to another
Means: Bacterial Conjugation
Need: To specifically control who can read the DNA message
Means: Riboregulation
Some slides borrowed from the 2006 Berkeley Team
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Regulatory Part/Promoter
Terminator
Protein
Protein Coding PartRibosome Binding
Site
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Ribosome Binding Site
Protein
DNA
RNARibosome Binding
Site
DNA to RNA to Protein
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Protein synthesis from RNA
Protein
RNARibosome Binding
Site
Ribosome
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Locked structure prevents ribosome binding
Protein
RNARibosome Binding
Site
Ribosome
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RNA “Key” unlocks lock
Protein
RNARibosome Binding
Site
Ribosome
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Goal: Create network of cell-cell communication
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RNA as a regulatory mechanism
• Can activate some “riboswitches” with small molecules
• Advantage: fast response - transcript is already made
• Alternative method: block translation by adding antisense RNA to RBD