Rocky Mountains Conference
EPR Workshop: DEER Analysis
programs testing
Sen I., Logan, T. and Fajer P., Biochemistry, 2007;46:11639-49.
http://fajerpc.magnet.fsu.edu/Programs/DEFit/defit.html
DEFit (for CW spectra:
CwdipFit)
• All spectra were provided by the anonymous symposium participants and analyzed blindly using DEFit (till today we don’t know what the spectra are)
• The spectrum naming (slide titles) follow the key provided by the panel moderator, Dr. Eric Hustedt.
• Hardware: Intel Duo Core2, Matlab 2007b
2a
•components A and B very well defined:narrow distributionsassymetric population
•component (c) badly defined component
C
A,B
3 Gaussians
2b
Noisy data => large error surfaceaverage distance better defined than the distribution
data0001
Excellent definition of three populations … complex assymetric population
data0002
one population, very well defined
data0003
•components A and B very well defined:narrow distributionsassymetric population
•component (c) badly defined component
last0001
last0002
Spikes are not always spikes! two components one very narrow
last0003
two components one very narrow
last0004
DATA1
low modulation depth, very long distance and very broad
DATA2
three components, chi2 impovement 6x from 2 components.one extremely sharp
