Production of Erythropoietin in Pichia
PastorisEporis – Team 15
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Team Eporis
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Abby Leistra Stephen Tubergen Zion Lee Nick Giles
ProblemBackgroundTeam Design NormsDesign Alternatives
Background: What is Erythropoietin?
•Erythropoietin (EPO) is a glycoprotein hormone•Promotes red blood cell production•Naturally made in kidneys•Treats anemia caused by• Chronic kidney disease
• Cancer chemotherapy
• HIV therapy
• $13 Billion Market
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glycam.org
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Problem
•An eight-week treatment can cost up to $11000
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• Expression: Chinese Hamster Ovary (CHO) Cells
http://nikon2.magnet.fsu.edu
• Well studied
• Expensive feed
• Slow growth rate
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Solution?
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• Expression: Pichia Pastoris cells
• Relatively new
• Cheap feed (methanol)
• Fast growth rate
2013.igem.org
ProblemBackgroundTeam Design NormsDesign Alternatives
Design Alternatives
•Pichia Pastoris Strain
• Turn on and off right genes
•Erythropoietin Structure
• Glycosylation and other modifications
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glycam.org
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Design Alternatives
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• Bioreactors
• Separation Units
Mianbin Wu, et. al. (2011). DOI: 10.5772/20246.
ProblemBackgroundTeam Design NormsDesign Alternatives
Design Norms
•Stewardship
•Transparency
•Trust
7/7ProblemBackgroundTeam Design NormsDesign Alternatives