Download - Preparing and imaging spiders in EtOH
![Page 1: Preparing and imaging spiders in EtOH](https://reader035.vdocuments.mx/reader035/viewer/2022081520/56816389550346895dd47923/html5/thumbnails/1.jpg)
Preparing and imaging spiders in EtOH
(like this one) ->
![Page 2: Preparing and imaging spiders in EtOH](https://reader035.vdocuments.mx/reader035/viewer/2022081520/56816389550346895dd47923/html5/thumbnails/2.jpg)
Preparing
Empty specimen(s) into petri dish
![Page 3: Preparing and imaging spiders in EtOH](https://reader035.vdocuments.mx/reader035/viewer/2022081520/56816389550346895dd47923/html5/thumbnails/3.jpg)
I prefer to pose spiders over sand. Fill the sand dish with 70% EtOH, enough to cover the specimen completely.
![Page 4: Preparing and imaging spiders in EtOH](https://reader035.vdocuments.mx/reader035/viewer/2022081520/56816389550346895dd47923/html5/thumbnails/4.jpg)
Gently transfer the specimen with forceps to the sand dish. You want the specimen completely submerged as to not get air bubbles in your final image.
(much unlike this photo)
![Page 5: Preparing and imaging spiders in EtOH](https://reader035.vdocuments.mx/reader035/viewer/2022081520/56816389550346895dd47923/html5/thumbnails/5.jpg)
Lens & Lighting
The 5D (using 65mm lens) along with the umbrellas positioned as shown in the next slide tend to work best.
So far all of my images have been taken with the 5D/65mm combo.
This is the 5D, with the 65mm lens at a 5.6 aperture.
![Page 6: Preparing and imaging spiders in EtOH](https://reader035.vdocuments.mx/reader035/viewer/2022081520/56816389550346895dd47923/html5/thumbnails/6.jpg)
Lens & Lighting continued
This is my lighting setup. So far it hasn’t failed me!
The Dynalite settings vary depending on the specimen & camera zoon.
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And now, the end result…. (big scary spider warning)
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Castianeira c.f. variata, dorsal
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Castianeira c.f. variata, ventral
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Drassylus mexicanus
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Habronattus icenoglei
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Hololena hola, dorsal
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Hololena hola, ventral
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Arctosa littoralis, dorsal
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Arctosa littoralis, ventral
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Synema sp. dorsal
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Synema sp. ventral
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Xysticus apachecus
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Xysticus gosiutus, dorsal
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Xysticus gosiutus, ventral