Download - Practical Microbiology
![Page 1: Practical Microbiology](https://reader035.vdocuments.mx/reader035/viewer/2022081416/56815638550346895dc3df22/html5/thumbnails/1.jpg)
Practical Microbiology
Ibrahim Mahmoud Sayed Ibrahim
![Page 2: Practical Microbiology](https://reader035.vdocuments.mx/reader035/viewer/2022081416/56815638550346895dc3df22/html5/thumbnails/2.jpg)
Instrument
Instruments
1-Seitz filter
2—MacKnotoch 3-Gas Pack system
![Page 3: Practical Microbiology](https://reader035.vdocuments.mx/reader035/viewer/2022081416/56815638550346895dc3df22/html5/thumbnails/3.jpg)
2 -IMVC test
•IMVC of E.coli •
![Page 4: Practical Microbiology](https://reader035.vdocuments.mx/reader035/viewer/2022081416/56815638550346895dc3df22/html5/thumbnails/4.jpg)
IMVC test
Citrobacter Klebsellia
![Page 5: Practical Microbiology](https://reader035.vdocuments.mx/reader035/viewer/2022081416/56815638550346895dc3df22/html5/thumbnails/5.jpg)
TSI (triple sugar iron)•LF (Ecoli) Pseudomonas Proteus
![Page 6: Practical Microbiology](https://reader035.vdocuments.mx/reader035/viewer/2022081416/56815638550346895dc3df22/html5/thumbnails/6.jpg)
Christen urea media Simmon Citrate
•Pink +ve proteus Green –ve Ecoli
•Yellow –ve Blue +ve Klebellia
![Page 7: Practical Microbiology](https://reader035.vdocuments.mx/reader035/viewer/2022081416/56815638550346895dc3df22/html5/thumbnails/7.jpg)
Sugar fermentation of Salmonella
•S.paratyphi B
•S.paratyphi A S.typhi
![Page 8: Practical Microbiology](https://reader035.vdocuments.mx/reader035/viewer/2022081416/56815638550346895dc3df22/html5/thumbnails/8.jpg)
Sugar fermentation of Shigella
•Sh.dysentriae Sh.flexenerii Sh.Sonneii
![Page 9: Practical Microbiology](https://reader035.vdocuments.mx/reader035/viewer/2022081416/56815638550346895dc3df22/html5/thumbnails/9.jpg)
Sugar Fermentation of Neisseria
•N.gonorrhea N.meningitidis
![Page 10: Practical Microbiology](https://reader035.vdocuments.mx/reader035/viewer/2022081416/56815638550346895dc3df22/html5/thumbnails/10.jpg)
Non-inoculated Media
•Loffler’s serum Dorset Egg Lowstein Jensen media
![Page 11: Practical Microbiology](https://reader035.vdocuments.mx/reader035/viewer/2022081416/56815638550346895dc3df22/html5/thumbnails/11.jpg)
Non inoculated media
TCBS Anaerobic media Thioglycolate –Robertson cooked meat-Deep agar
![Page 12: Practical Microbiology](https://reader035.vdocuments.mx/reader035/viewer/2022081416/56815638550346895dc3df22/html5/thumbnails/12.jpg)
II inoculated mediaNutrient Agar
Staph (endopigment) Pseudomonas (Exopigment)
Proteus (swarming) Sabroud dextrose (yeast)
![Page 13: Practical Microbiology](https://reader035.vdocuments.mx/reader035/viewer/2022081416/56815638550346895dc3df22/html5/thumbnails/13.jpg)
II-Blood agar•Blood agar (B-hemolysis) as Staph aureus, Strept Pyogens
•Blood tellurite as Corynebacterium diphtheriae
![Page 14: Practical Microbiology](https://reader035.vdocuments.mx/reader035/viewer/2022081416/56815638550346895dc3df22/html5/thumbnails/14.jpg)
III Chocholate agar•Chocholate agar Optochin sensitivity
• Pneumococci +ve zone : Pneumococci•- ve zone : S.viridans
![Page 15: Practical Microbiology](https://reader035.vdocuments.mx/reader035/viewer/2022081416/56815638550346895dc3df22/html5/thumbnails/15.jpg)
IV) Pink Plate •Mannitol salt agar MacConkey Agar
• Staph aureus Lf (Ecoli)
![Page 16: Practical Microbiology](https://reader035.vdocuments.mx/reader035/viewer/2022081416/56815638550346895dc3df22/html5/thumbnails/16.jpg)
V) EMB plate
•E.coli Klebsellia
![Page 17: Practical Microbiology](https://reader035.vdocuments.mx/reader035/viewer/2022081416/56815638550346895dc3df22/html5/thumbnails/17.jpg)
VI) Special Plate•MacConkey Agar Sabroud dextrose Agar
•NLF(Salmonella) Mould
![Page 18: Practical Microbiology](https://reader035.vdocuments.mx/reader035/viewer/2022081416/56815638550346895dc3df22/html5/thumbnails/18.jpg)
VI) Special Plate
•Milk Agar Bismuth Sulphite•Staph Salmonella