Planning rice breeding programs for impact
Pedigree Selection
in the IRRI Irrigated Breeding Program
Parminder S. Virk
PBGB Division
IRRI: Planning Breeding Programs for Impact
Can anyone define “pedigree”?
IRRI: Planning Breeding Programs for Impact
• Describe the pedigree method
• Describe naming of pedigree at IRRI
• Describe crosses at IRRI
• Describe types of crosses
• Demonstrate IRRI nurseries
Learning objectives
IRRI: Planning Breeding Programs for Impact
The pedigree method owes its name to the pedigree record of selected plants maintained to trace ancestral relationship among such plants
A detailed description of present day pedigree method was outlined by Love (1927)
Pedigree Method
IRRI: Planning Breeding Programs for Impact
In this method individual plants are selected starting with F2
and in the subsequent segregating generations their progenies are tested till the progenies become homozygous.
During entire operation a record of all parent-offspring relationship is kept: PEDIGREE RECORD
In this method each progeny in every generation can be traced back to the original F2 plant
Pedigree describes parents, grandparents,
great-grandparents,...
Pedigree Method
IRRI: Planning Breeding Programs for Impact
Based on cross number and serial numbers of selected plants
IR numbers are assigned consecutively to the crosses (now IR80389) made at IRRI just before the hybrid seeds are incubated for germination
When plant selections are made from F2 and
subsequent generations of an IR cross, numerical designations are given for each breeding line
How is the naming of pedigree at IRRI?
IRRI: Planning Breeding Programs for Impact
Selections from the F2 were grown in the pedigree nursery as F3 rows:
IR2061-1, ------IR2061-214 -----and so on up to IR2061-806
From selection 214, three plants were grown as F4 selections:
IR2061-214-1, IR2061-214-2 and IR2061-214-3
From selection IR2061-214-3, 10 plants were again selected as F5 rows
These selections were designated as IR2061-214-3-1 up to 2061-214-3-10
From selection IR2061-214-3-8, three plant selections were made and planted in the pedigree nursery as F6 rows IR2061-214-3-8-1, IR2061-
214-3-8-2 and IR2061-214-3-8-3
At maturity selection IR2061-214-3-8-2 appeared uniform and was bulk harvested and evaluated in replicated trials
Example: IR2061-214-3-8-2 (IR28)
IRRI: Planning Breeding Programs for Impact
How is the naming of pedigree in your country/institute?
IRRI: Planning Breeding Programs for Impact
Procedure
What are the breeding objectives? • High Yield Potential• Short Growth Duration• Superior Grain Quality• Multiple disease and insect resistance
Choice of Parents?
IRRI: Planning Breeding Programs for Impact
Staggered sown 3 times each season
at 2-weeks interval
IRRI Varieties are donors in various programs as
Irrigated
Rainfed
Flood prone and
Hybrid rice
Donors for various traits (Hybridization Block)
IRRI: Planning Breeding Programs for Impact
Hybridization Block
IRRI: Planning Breeding Programs for Impact
Hybridization Block
IRRI: Planning Breeding Programs for Impact
Field Book
IRRI: Planning Breeding Programs for Impact
Summary of HBsENTRYNO DESIGNATION BL B1 RTV BPH1 GLH GELCON GT AMY GRL GRS CLK
HB8401 IR 20 7 1 87 8 8 44 L 25 5 5 2HB8402 IR 29 6 1 60 4 4 94 L 2 3 1 0HB8403 IR 36 6 1 81 2 6 42 HI/I 25 3 1 3HB8404 IR 43 6 1 85 8 7 81 L 18 3 3 5HB8405 IR 58 5 1 58 2 4 34 I/L 26 5 5 1HB8406 IR 64 5 1 71 3 5 77 I 23 3 1 4HB8407 IR 72 7 1 75 2 4 78 I/L 26 3 1 2HB8408 IR 74 3 6 79 2 4 51 L 26 3 1 5HB8409 PSB RC 10 2 1 85 2 4 56 I/L 25 3 1 4
Electronic Field Book
IRRI: Planning Breeding Programs for Impact
IR 65610-24-3-6-3-2-3
IR 67966-44-2-3-2IR 68544-29-2-1-3-1-2
IR 68552-100-1-2-2IR 76910-20-6 (xa 5,xa 13,Xa 21 )IRBB 60-1
IR 69726-116-1-3IR 73885-1-4-3-2-1-10IR 73887-1-8-2-1IR 73888-1-2-7
BG 90-2IR 45912-9-1-2-2IR 65620-192-3-3-3-2IR 68440-36-2-2-3
Selected Donors
IRRI: Planning Breeding Programs for Impact
Planning Crosses!
IRRI: Planning Breeding Programs for Impact
For Yield, grain quality and incorporation of resistance to diseases and insects
Released varieties x Elite Indica Lines
Elite Indica Lines x Elite Indica Lines
Elite Indica Lines x Basmati Type Lines
Further improvement in Indica germplasm
IRRI: Planning Breeding Programs for Impact
Basmati x Elite Indica Lines
Basmati x Basmati Type Derivatives
Basmati Type Derivatives x Basmati Type Derivatives
Developing Basmati Type Germplasm
IRRI: Planning Breeding Programs for Impact
Tropical Japonica x Tropical Japonica crosses
Tropical Japonica x Korean Varieties
Improving Tropical Japonicas for temperate regions (Korea)
IRRI: Planning Breeding Programs for Impact
For Yield, grain quality and incorporation of resistance to diseases and insects
Improved NPTs x Improved NPTs
Improved NPTs x Released Indica Varieties
Improved NPTs x Elite Indica Varieties
Further Improvement in NPTs
IRRI: Planning Breeding Programs for Impact
What crosses do you currently focus on in
your institute?
IRRI: Planning Breeding Programs for Impact
Types of crosses:
Single crossesBackcrossesThree way/Top crosses
Around 900 crosses yearly (wet & dry season)
Cross designation (IR80369)
F1 Nursery
IRRI: Planning Breeding Programs for Impact
IRRI: Planning Breeding Programs for Impact
F1
IRRI: Planning Breeding Programs for Impact
Grow 2-6 rows (30 Plants) /cross
Rouge off-types
Identify accidental selfs
Field Operations
IRRI: Planning Breeding Programs for Impact
Protected:In general susceptible to diseases and insects
Unprotected:In general resistant to diseases and insects
First grow in blast nursery &Reject Susceptible populations
Space planted 35 x 25 cm.Population size from 2,500- 5,000 individuals
F2 Nursery
IRRI: Planning Breeding Programs for Impact
Blast Nursery
IRRI: Planning Breeding Programs for Impact
Blast Nursery
IRRI: Planning Breeding Programs for Impact
Blast Nursery
IRRI: Planning Breeding Programs for Impact
Protected F2
IRRI: Planning Breeding Programs for Impact
Unprotected F2
IRRI: Planning Breeding Programs for Impact
•Inoculate with BB Race1/Race2
•Rogue susceptible/undesirable plants
•Harvest desirable plants (100-500 plants)
Field Operations
IRRI: Planning Breeding Programs for Impact
Four nurseries/year: • January & July (Unprotected)• May & November (Protected)
Space planted 30 x 20 cm Every 20th row is a check variety (4 Checks) 16,000 to 20,000 rows (30 plants)
Pedigree nursery
What system do you have in your institute?
IRRI: Planning Breeding Programs for Impact
Pedigree Nursery in May
IRRI: Planning Breeding Programs for Impact
Pedigree Nursery in May
IRRI: Planning Breeding Programs for Impact
Pedigree Nursery in July
IRRI: Planning Breeding Programs for Impact
Pedigree Nursery in July
IRRI: Planning Breeding Programs for Impact
Screening requirementsMemo
DATE : 2002-04-23 PN MAY 2002 SCREENING REQUIREMENTS
Cross Parents Start End Sel. Gen.GL B
AR GEL
AM
GR
1 2 3 H L 1 2 O TEMP Y EIR 74713 IR 69807-13-2-1 80001 80003 3 F9
IR 69715-36-1-1IR 75261 IR 65600-38-1-2-1 80004 80006 3 F8
PSB RC 64IR 75263 IR 66158-38-3-2-1 80007 80012 6 F8
IR 68058-64-1-2IR 75266 IR 66158-38-3-2-1 80013 80015 3 F8
IR 68450-36-3-2-2-3
X
B P H B B
X X X
X X X X X
X
X
X X X X
X
X
XX X X X
IRRI: Planning Breeding Programs for Impact
Field Book
IRRI: Planning Breeding Programs for Impact
F3 single rows
F4 onwards 3/6 rows
Selection between rows/within rows- at least 3 plants.
IRRI: Planning Breeding Programs for Impact
17811 Progeny RowsComposition of 2004 NOV PN
Gen Cross type No. Gen Cross type No.
F3 I/I/I 4 F5 I/I 5
IND/IND 18 I/I/I 2
IND/WH 3 I/J/I 18
JAP/BAS/ARO 4 J/I 6
JAP/I/I 1 J/J 3
Jap/Korean 10 Micronutrient 21
NPT/IND 19 F6 I/I 17
NPT/JAP/IND 3 NPT/I 32
F4 I/I 11 NPT/NPT 17
I/I/I 1 J/I/I 18
I/J 31 Micronutrient 40
NPT/BAS 6 F7 I/J 2
NPT/I/I 5 Micronutrient 20
NPT/NPT 14
NPT/NPT/I 3
IRRI: Planning Breeding Programs for Impact
Cross Parents Start EndSel
.Gen
.Type
IR 79228 IR 72903-6-2-2-1 46001 46006 6 F7 I/J
IR 67966-44-2-3-2
IR 79232 IR 72905-49-3-2-2 46007 46009 3 F7 I/J
IR 68552-100-1-2-2
IR 79483 IR 71700-247-1-1-2 46010 46015 6 F6 I-NPT/NPT
IR 68544-29-2-1-3-1-2
IR 79484 IR 71700-247-1-1-2 46016 46018 3 F6 I-NPT/NPT
IR 72225-20-3-2-3
IR 79485 IR 71700-247-1-1-2 46019 46037 18 F6 I-NPT/NPT
IR 73707-45-3-2-3
IR 79487 IR 72158-11-5-2-3 46038 46050 12 F6 I-NPT/NPT
IR 72225-20-3-2-3
IR 79489 IR 72967-12-2-3 46051 46053 3 F6 I-NPT/NPT
IR 68544-29-2-1-3-1-2
IR 79493 IR 67961-15-1-1 46054 46072 18 F6 NPT/NPT
IR 68544-29-2-1-3-1-2
IR 79494 IR 67961-15-1-1 46073 46082 9 F6 NPT/NPT
IR 72225-29-1-2-1
2004 NOV PN
IRRI: Planning Breeding Programs for Impact
OYT
IRRI: Planning Breeding Programs for Impact
RYT
IRRI: Planning Breeding Programs for Impact
Any questions or comments?