Download - Nicholas lab: C. elegans research
Nicholas lab: C. elegans research
Working with wormsPostgraduate induction 2013
Yee Lian Chew
C. elegans – the roundworm
• Small – 1 mm long in adults• Anatomy has been fully mapped • Highly stereotypical• Reproduce sexually by mating with
males and also by self-fertilisation (maintained as a haermaphrodite population)
• Short-lived • Transparent
What we do
Matthew
Mallory
Priya
Irene
Anna
DuyguHannah Sashi
Hong
Yee Lian
Aaron
Estelle
Slavica
C. elegans – techniques we use
• Generation of transgenic lines – biolistic transformation and microinjection
• Molecular biology techniques including DNA/RNA work, qPCR, RNAi
• Staining, western blot, mass spec (iTRAQ)• Microscopy – EM, confocal, fluorescence,
dissecting scope• Dissections – germline staining• And of course, lots of fun with worms
Worms are transparent – and yes, they have brains
They have 302 neurons
Co-localisation studies using transgenic C. elegans
pdpy-7::HIS-24::mCherry
pctbp-1::ctbp-1::gfp
Merge
Matthew Hoe