Next Generation
Lectins
Recombinant Lectins Ensure Stable Supplies
Ready-to-Use Lectin Solution
For Glycobiology & Cell Biology
Wako has been developing lectins for sugar chain research, aiming to ensure those stable supplies. The first launch is recombinant BC2LCN (AiLecS1) for research of human induced pluripotent stem (iPS) and embryonic stem (ES) cells. rBC2LCN (AiLecS1) is expected to be applicable to not only stem cell research but also to regenerative medicine since it is reportedly a useful marker for detection of undifferentiated cells.
rBC2LCN-FITC DAPI MERGE
Apr. 2016
Application - Detections of an undifferentiation marker of human iPS cells
Wako Pure Chemical Ind., Ltd. www.wako-chem.co.jp
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■ rBC2LCN (AiLecS1) from cDNA of Burkholderia cenocepacia
OCT3/4
TRA-1-81
rBC2LCN -Cy3
rBC2LCN -FITC
BSA-Cy3
BSA-FITC
DAPI MERGE
rBC2LCN 029-18061 (1mg), 025-18063 (1mg×5)
Glycan specificity Fucα1-2Galβ1-3GalNAc (H type 3) Fucα1-2Galβ1-3GlcNAc (H type 1)
Formulation PBS (pH 7.4)
cDNA source Synthetic cDNA based on Burkholderia cenocepacia
Protein concentration
1.2~1.8mg/ml (BCA Assay)
Source E. coli expressed Burkholderia cenocepacia lectin (BC2LCN/AiLecS1)
Molecular weight (theoretical value)
15,807
Purification Affinity purification by fucose agarose. Storage condition Keep at -20℃ before use. After thawing, keep at 2-10℃. Avoid the freeze-thaw cycle.
Product Outline
Detection of undifferentiated iPS cells using rBC2LCN
rBC2LCN References
rBC2LCN, a marker of undifferentiated human ES・iPS cells
Point in Check
rBC2LCN reportedly recognized an intramolecular sugar chain of Podocalyxin, exclusively, which exposes on the cell surface of undifferentiated human iPS cells. Please see the reference 1).
rBC2LCN Cy3
rBC2LCN FITC
DAPI MERGE BF
SSEA-4
TRA-1-81
<Detection condition> Fluorescent labeled rBC2LCN Final concentration : 0.1-0.5 μg/mL (1w/v% BSA, 1×PBS) Incubation time : 60 min. (RT, in the dark) Cell : Human iPS 201B7 strain Fixing condition: 4w/v% Paraformaldehyde for 10min. at RT or 4℃.
Detection of undifferentiated marker in human iPS cell colony naturally differentiated
Point in Check rBC2LCN (rLectin)
Anti TRA-1-81 Mab (Mouse IgM)
Anti SSEA-4 Mab (Mouse IgG3)
M. W. (kDa) 16 900 150
Flow Cytometry ○ ○ ○
Cytological staining ○ ○ ○
Live cell imaging ○ △ - Non-specific binding (Cytological staining)
Low High Low
1) Tateno, H., Matsushima, A., Hiemori, K., Onuma, Y., Ito, Y., Hasehira, K., Nishimura, K., Ohtaka, M., Takayasu, S., Nakanishi, M., Ikehara, Y., Nakanishi, M., Ohnuma, K., Chan, T., Toyoda, M., Akatsu, H., Umezawa, A., Asashima, M. and Hirabayashi, J. “Podocalyxin is a Glycoprotein ligand of the human pluripotent stem cell-specific probe rBC2LCN.”, Stem Cells Translational Medicine, 2(4), 265-73(2013). 2) Onuma, Y., Tateno, H., Hirabayashi, J., Ito, Y. and Asashima, M. “rBC2LCN, a new probe for live cell imaging of human pluripotent stem cells.”, Biochem. Biophys. Res. Commun., 431(3), 524-9(2013). 3) Tateno, H., Toyota, M., Saito, S., Onuma, Y., Ito, Y., Hiemori, K., Fukumura, M., Matsushima, A., Nakanishi, M., Ohnuma, K., Akutsu, H., Umezawa, A., Horimoto, K., Hirabayashi, J. and Asashima, M. “Glycome diagnosis of human induced pluripotent stem cells using lectin microarray.”, J. Biol. Chem., 286(23), 20345-53(2011). 4) Tateno, H., Onuma, Y., Ito, Y., Hiemori, K., Aiki, Y., Shimizu, M., Higuchi, K., Fukuda, M., Warashina, M., Honda, S., Asashima, M. and Hirabayashi, J. “A medium hyperglycosylated podocalyxin enables noninvasiva and quantitative detection of tumorigenic human pluripotent stem cells.”, Sci. Rep., 4, 4069(2014).
*1: Lectin group means one kind of classification of expediency based on the glycan specificity. It doesn’t warrant the recognition ability of all glycans.
*2: The information of the glycan specificity are the common information. These don’t limit the specificity of lectins.
Lectin Groups*1 Name Code No. Package
Size
cDNA Source
Glycan Specificity *2
Fucose-
Complex
BC2LCN
(AiLecS1)
029-18061
025-18063 1mg
1mg×5
cDNA Source : Burkholderia cenocepacia
Target Glycan : Fucα1-2Galβ1-3GalNAc (H type 3), Fucα1-2Galβ1-3GlcNAc (H type 1)
AAL 018-25201
014-25203 1mg
1mg×5
cDNA Source : Aleuria aurantia
Target Glycan : Fucose moieties
PA-IIL 160-26711
166-26713 1mg
1mg×5
cDNA Source : Pseudomonas aeruginosa
Target Glycan : Fucose, Fucose containing oligosaccharides, Mannose
RS-Fuc 183-03101
189-03103 1mg
1mg×5
cDNA Source : Ralstonia solanacearum
Target Glycan : Fucose
Glc/GlcNAc-
Complex
F17AG 062-06281
068-06283 1mg
1mg×5
cDNA Source : E. coli
Target Glycan : GlcNAc
Malectin 136-18221
132-18223 1mg
1mg×5
cDNA Source : Human
Target Glycan : Glc2-N-biose
Gal/GalNAc-
Complex
ABA 015-24851
011-24853 1mg
1mg×5
cDNA Source : Agaricus bisporus
Target Glycan : Galβ1-3GalNAc (T antigen), Galβ1-3GlcNAc (Lacto-N-biose)
CGL2 033-23771
039-23773 1mg
1mg×5
cDNA Source : Coprinopsis cinerea
Target Glycan : βGal, GalNAcα1-3Gal (Blood Group A), Galα1-3Gal (Blood Group B)
CNL 039-23631
035-23633 1mg
1mg×5
cDNA Source : Clitocybe nebularis
Target Glycan : α/βGalNAc, GalNAcβ1-4GlcNAc,
GalNAcα1-3[Fucα1-2]Galβ1-4GlcNAc (Blood Group A)
Discoidin I 045-33541
041-33543 0.5mg
0.5mg×5
cDNA Source : Dictyostelium discoideum
Target Glycan : αGalNAc (Tn antigen)
Discoidin II 042-33431 0.5mg cDNA Source : Dictyostelium discoideum
Target Glycan : Gal, LacNAc, Asialoglycans, Gal/GalNAcβ1-4GlcNAcβ1-6Gal/GalNAc
Gal1-S 072-06341
078-06343 1mg
1mg×5
cDNA Source : Human
Target Glycan : branched LacNAc
Gal1, Stable Form 070-06401 50μg cDNA Source : Human
Target Glycan : branched LacNAc, Gal
Gal2 073-06371 1mg cDNA Source : Human
Target Glycan : GalNAcα1-3Gal (Blood Group A), branched LacNAc
Gal3C-S 079-06351
075-06353 1mg
1mg×5
cDNA Source : Human
Target Glycan : poly LacNAc
Gal3(Full-length) 077-06411 50μg cDNA Source : Human
Target Glycan : poly LacNAc
Gal7-S 076-06361
072-06363 1mg
1mg×5
cDNA Source : Human
Target Glycan : Galβ1-3GlcNAc
Gal9, Stable Form 074-06421 50μg cDNA Source : Human
Target Glycan : poly LacNAc, GalNAcα1-3Gal (Blood Group A)
LSL-N 127-06361
123-06363 1mg
1mg×5
cDNA Source : Laetiporus sulphureus
Target Glycan : LacNAc, poly LacNAc
MOA 137-18011
133-18013 1mg
1mg×5
cDNA Source : Marasmius oreades
Target Glycan : Galα1-3[Fucα1-2]Galβ1-4GlcNAc (Blood Group B)
Galα1-3Galβ1-4GlcNAc, Galα1-3Gal
PA-IL 167-26721
163-26723 1mg
1mg×5
cDNA Source : Pseudomonas aeruginosa
Target Glycan : Galα1-3(4)Gal
PPL 168-26751 0.5mg cDNA Source : Pleurocybella porrigens
Target Glycan : α/βGalNAc
SRL 199-17271
195-17273 1mg
1mg×5
cDNA Source : Sclerotium rolfsii
Target Glycan : Galβ1-3GalNAc (T antigen), Galβ1-3GlcNAc (Lacto-N-biose)
XCL 241-00971 1mg cDNA Source : Xerocomus chrysenteron
Target Glycan : Galβ1-3GalNAc/GlcNAc
Sialic Acid
ACG 011-25431
017-25433 1mg
1mg×5
cDNA Source : Agrocybe cylindracea
Target Glycan : α2-3 Sialic Acid
PSL1a 161-26741
167-26743 0.5mg
0.5mg×5
cDNA Source : Polyporus squamosus
Target Glycan : α2-6 Sialic Acid
Mannose
-Complex
BanLec 025-18661 0.5mg
cDNA Source : Musa acuminate
Target Glycan : α-Mannan, α-Glucan (Recognize internal α1-3 linked
glucosyl residues. Not recognize α-1-4, 1-6 linkage)
BC2L-A 026-18691 1mg cDNA Source : Burkholderia cenocepacia
Target Glycan : High-mannose
Calsepa 031-23831
037-23833 1mg
1mg×5
cDNA Source : Calystegia sepium
Target Glycan : High-mannose
GRFT 070-06381
076-06383 1mg
1mg×5
cDNA Source : Griffithia sp.
Target Glycan : High-mannose
Heltuba 086-10331 1mg cDNA Source : Helianthus tuberous
Target Glycan : Mannose
PALa 164-26731
160-26733 1mg
1mg×5
cDNA Source : Phlebodium aureum
Target Glycan : High-mannose
Orysata 159-03281
155-03283 1mg
1mg×5
cDNA Source : Oryza sative
Target Glycan : High-mannose
【Product Outline】
○Formulation:Liquid
○Source:Each lectin cDNA (synthetic) expressed in E. coli.
○Storage Condition:-20℃ and/or -80℃ After thawing, keep at 2-10℃. Avoid the freeze-thaw cycle.
16400開110
Description Code No. Package Size Glycan Specificity
Concanavalin A [Con A]
037-08771
033-08773
031-08774
100mg
250mg
1g
Man
Datura Lectin, Lyophilized [DSA] 047-23231 5mg Glu, GlcNAc
Dolichos Biflorus Lectin, Peroxidase Labeled [DBA] 047-23731 1mg GalNAc
Maackia Amurensis Lectin, Lyophilized [MAM] 139-10891 2mg Gal, GalNAc
Peanut Lectin, Lyophilized [PNA] 165-15031 5mg Gal, GalNAc
Phytohemagglutinin-E4, Lyophilized [PHA-E4] 164-15241 5mg Glc, GlcNAc
Phytohemagglutinin-L4 [PHA-L4] 168-15261 5mg Glc, GlcNAc
Phytohemagglutinin-P [PHA-P] 161-15251 50mg Glc, GlcNAc
Sambucus Sieboldiana Lectin, Lyophilized [SSA] 197-10371 5mg Siaα2-6Gal, Siaα2-6GalNAc
Soybean Lectin, Lyophilized 198-09931 5mg Gal, GalNAc
Wheat Germ Lectin [WGA] 126-02811 10mg Glc, GlcNAc
Description Code No. Package Size Clone No. Host Species Isotype Reference
Anti Siaα2-3, Monoclonal Antibody
recognition region: GM3, GM4, α2-3nLc4Cer
011-25171 200μL HYB4 Mouse IgG3・κ 1) Hidari, K. et al., BBRC, 436, 394
(2013).
Anti Keratan Sulfate, Monoclonal Antibody
recognition region: Keratan sulfate (lacking
oversulfated structure)
011-25811
017-25813
200μL
1mL R-10G Mouse IgG1
1) Kawabe, K. et al., Glycobiology, 23(3),
322 (2013).
Anti Sialyl-Tn Antigen, Monoclonal Antibody
recognition region: A cluster composed of Siaα2-
6GalNAc-Ser/Thr (Sialyl Tn),
Mucin-type glycoprotein
010-25881
016-25883
100μL
500μL MLS132 Mouse IgG3
1) Fukui, S. et al., Jpn. J. Cancer Res.,
79, 1119 (1988).
2) Numata, Y. et al., BBRC, 170(3), 981
(1990).
3) Nakada, H. et al., BBRC, 187(1), 217
(1992).
4) Nakada, H. et al., Proc. Natl. Acad.
Sci. USA, 90, 2495 (1993).
5) Tanaka, H. et al., Eur. J. Biochem.,
263, 27 (1999).
6) Akita, K. et al., Int. J. Gynecol.
Cancer, 22, 531 (2012).
Anti Tn Antigen, Monoclonal Antibody
recognition region: A cluster composed of
GalNAc-Ser/Thr (Tn antigen)
017-25891
013-25893
100μL
500μL MLS128 Mouse IgG3
Anti Sialyl-Lea Antigen, Monoclonal Antibody
recognition region: Siaα2-3Galβ1-3[Fucα1-4]GlcNAc
(Sialyl Lea), Siaα2-3Galβ1-3GlcNAc
010-25901
016-25903
100μL
500μL MSW113 Mouse IgG3
1) Kitagawa, H. et al., J. Biochem., 104,
817 (1988).
2) Kitagawa, H. et al., J. Biol. Chem.,
268(35), 26541 (1993).
3) Akita, K. et al., Int. J. Gynecol.
Cancer, 22, 531 (2012).
Antibodies to Carbohydrate Antigen
Storage Condition
: 2~10℃
: -20℃
: -80℃
TRA-1-81
R-10G
DAPI Merge Phase
DAPI Merge Phase
Detection of undifferentiated iPS cells using R-10G antibody
<Detection condition> Primary Antibody : Upper Section : TRA-1-81 antibody as positive control Lower Section : Anti Keratan Sulfate antibody (clone No. R-10G) as test Secondary Antibody : FITC labeled secondary antibodies Cells: Human iPS cells 201B7 strain. Cultured on the feeder cells.
Bulk Supply is Available! Let’s contact with WAKO.