Maximize your HPLC
Efficiency
InfinityLab LC Series
Gregory Hunlen
Application Scientist
November 2016
1
The Agilent InfinityLab LC Series!
1260 Infinity II LC1290 Infinity II LC 1220 Infinity II LC
Sept. 2014 May 2016 Oct 2016
Agilent is completely renewing it’s analytical LC portfolio!
November 2016
2
InfinityLab LC Series – a Remarkable Pedigree
Evolution not Revolution – but with revolutionary technology
To be consistent with existing workflows is of utmost importance for most of our customers.
Agilent strictly focus to introduce innovative and revolutionary technologies always with backwards
compatibility in mind. Backwards compatible with existing modules and with methods!
November 2016
3
New Benchmarks in Efficiency
The Agilent 1290 Infinity II LC, the next generation in Ultra High
Performance Liquid Chromatography, raises the efficiency of your
business in three dimensions:
Maximize Analytical Efficiency
Unmatched separation and detection performance deliver analysis data of
the highest quality—for ultimate confidence in your results.
Maximize Instrument Efficiency
Highest sample capacity and fastest injection cycles combine with new
levels of usability—for highest throughput for any application.
Maximize Laboratory Efficiency
Seamless integration in current infrastructure and smooth method transfer
from legacy equipment—for non-disruptive transition to highest productivity
and lowest cost of ownership.
Agilent 1290 Infinity II LC
November 2016
4
New 1290 Infinity II VWD
New 1290 Infinity II Multisampler
New 1290 Infinity II High Speed Pump
New 1290 Infinity II Flexible Pump New Accessories New 1290 Infinity II ELSD
New 1290 Infinity II Multicolumn Thermostat New 1290 Infinity II DAD
New 1290 Infinity II DAD-FS
The New Agilent 1290 Infinity II LC
November 2016
5
Flexibility for all applications
1290 Infinity II Flexible/High Speed Pump
• Wide power range
• Gradient options for higher speed or flexibility
1290 Infinity II Multisampler
• Dual-needle setup for scalable injection volumes
1290 Infinity II Multicolumn Thermostat
• Column temperature range from 4 to 110 °C
Intelligent System Emulation
Technology to run legacy
methods – even from other
vendor’s LCs
November 2016
6
Wide power range up to 1300 bar (2ml/min)/800 bar
(5ml/min) for optimum use of all analytical columns
independent of inner-diameter or particle technology
Flexibility for all applications
November 2016
7
1290 Infinity II Pumps Comparison
8
1290 Infinity II High Speed Pump
• 2 Channels
• Power Range: 2ml/min (1300bar), 5ml/min
(800bar)
• 45µl Standard Delay Volume
• For ultrafast gradients
• Best front-end for MS Users (2.1mm ID
col., low flow)
• Top-choice for binary pump lovers
• Includes ISET
1290 Infinity II Flexible Pump
• 4 Channels
• Power Range: 2ml/min (1300bar), 5ml
(800bar)
• <350µl Standard Delay Volume
• Flexibility in gradient formation
• Buffer blending
• Method development
• No ultrafast methods, where delay volume
is critical!
• Unmatched accuracy and application
range
November 2016
Flexibility for all applications
Highest retention time precision for reliable
peak identificationThe 1290 Infinity II Flexible Pump is the only low-
pressure mixing pump that combines the ultimate
performance of a high-pressure mixing UHPLC pump with
the flexibility of a quaternary pump. This pump delivers
superior gradient performance over the entire composition
range from 1 to 99 %.
November 2016
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Highest retention time precision for reliable
peak identification
Isocratic analysis
The 1290 Infinity II Flexible Pump is the only low-
pressure mixing pump that combines the ultimate
performance of a high-pressure mixing UHPLC pump with
the flexibility of a quaternary pump. This pump delivers
superior gradient performance over the entire composition
range from 1 to 99 %.
November 2016
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Flexibility for all applications
The dual-needle setup of the 1290 Infinity II Multisampler
enhances flexibility by providing two injectors in a single
instrument. You can optimize one path for large volume
injections and the other for low delay volumes.
20µl
500µl
November 2016
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Lowest carryover for highest data quality
Wash inner needle
Flow through principle washes inner needle
thoroughly and constant with eluent.
Wash outer needle
Choice of 3 external solvents to program
washing steps for the outer needle.
Needle-seat back flush
Choice of 3 external solvents to back
flush the seat.
Using the new Multiwash feature you can flush the inside
and outside surfaces of the injection needle with up to three
different solvents, as well as backflush the needle seat.
November 2016
12
Lowest carryover for highest data quality
The 1290 Infinity II Multisampler with multiwash reduces
carryover to less than 9 ppm!
November 2016
13
How is Sample Management facilitated?
Agilent’s new Sample Entry Component uses intuitive graphical user interaction
similar to graphic/paint programs which allow sample list and sequence setups
based on sample container layouts. The component is autosampler and sample
container independent and can adapt to any sampler or container and geometry
via usage of the build-in and published proprietary description interfaces. For any sampler or container type
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Simple and effectively to use Graphical tool for sample entry
July 1, 2016
The NEW Agilent InfinityLab LC Series - India
Laboratory Efficiency – Invest into new LCs and software to finally save money!
The new Agilent 1260 Infinity II LC!
November 2016
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The new Agilent 1260 Infinity II LC!
New Electronics (future proof, RoHS compatible)
Leveraging 1290 Infinity II LC technologies into the core platform, for your benefit!
New Housing (better ergonomics, higher safety
standard)
New Autosamplers (significantly better performance)
New Column compartments (highest usability or
price optimized)
New Detectors (improved specifications)
New Pumps (new functions and features)
New Accessories (better usability, better performance)
With full backwards method compatibility!
November 2016
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November 2016
1260 Infinity II LC: 18 New Modules
17
* Available for ordering 01.Aug.2016
** Available for ordering Q1 2017
1260 Infinity II VWD
1260 Infinity II
Multisampler
1260 Infinity II
Binary Pump*
1260 Infinity II
Quarternary Pump
1260 Infinity II
Multicolumn Thermostat
1260 Infinity II DAD-HS
1260 Infinity II DAD-WR1260 Infinity II
Vialsampler
1260 Infinity II Bio-
inert Multisampler
1260 Infinity II Bio-inert
Quarternary Pump
1260 Infinity II FLD
1260 Infinity II RID
1260 Infinity II
Isocratic Pump
1260 Infinity II MWD
1260 Infinity II
SFC Multisampler**
1260 Infinity II
SFC Binary Pump**
1260 Infinity II FLD Spectra
1260 Infinity II
ELSD*
Infinity II Vialsampler
• drawer-like vial-trays with convenient access
• 2 individual trays for loading during analysis
• 2 x 66 vials = 132 vials
(cartesian arrangement A1-F11)
• Optional „classic vial tray 100“
(numbering 1-50 and 51-100)
Vial trays (default = 2x 66 vials 2 mL)
Needle-wash Station
• Outer Needle Wash for carryover reduction
• Carryover <40 ppm
18
November 2016
Infinity II Vialsampler: integration
Sample Cooler option
• 5°C below ambient down to 4°C
• Accuracy +/- 2°C
• Slide in cooler from backside
• Customer installable
• No heating capability
Integrated Column Compartment (ICC) option
• Optional upgrade, user exchangable
• Column heating (+5°C above ambient to 80°C)
• up to 2 Columns (max 7 mm ID, max 30 cm lenght)
• 2 Versions: ICC with 3 µl or 6 µL heater volume
• Hinged door for convenient column installation
19
November 2016
Infinity II Vialsampler: easy access
Walk-Up option
• External tray Option
• up to 5 vials
• Automated loading and diposal
• Supported by OpenLAB and MassHunter
20
November 2016
Laboratory Efficiency – Invest into new LCs and software to finally save money!
The new Agilent 1220 Infinity II LC!
November 2016
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The Agilent 1220 Infinity II LC600 bar for UHPLC Capabilities!
Isocratic or Gradient Pump:• 600 bar
• flow range: 0.2 - 10 mL/min
• low pressure mixing
• integrated degasser
• flow precision 0.07 % RSD
• AIV available NEW
Manual Injector or Autosampler:• injection range: 0.1-100 µL, extension up to
1500 µL
• 100 x 2 mL vials or 30 x 6 mL vials
• injection cycle time 50 s
• carryover: < 0.05%
• SIM sampler cooler 5-40°C coming soon
Column Oven:• up to 80°C
• one column, max. 250 mm
VWD or DAD:• 80 Hz data rate
• wavelength range: 190 - 600 nm (VWD)
up to 950 nm (DAD)
• 2.5 µAU (7 µAU for DAD) noise at 230 nm
November 2016
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1220 Infinity II LC Mobile SolutionThe only transportation-optimized UHPLC system in the world !!!
The 1220 Infinity II LC in combination with a Mobile
Upgrade Kit for shock & vibration resistance.
It can be placed in a van for on-site LC analysis.
November 2016
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100% Method TransferabilitySwitching from 1100 Series to 1220/1260 Infinity LC
100% HPLC compatibility: On one system you can run your established and
validated HPLC methods – unchanged – as well as push UHPLC to new levels of
performance.
November 2016
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The Story of the Infinity II Series
is the Story of Efficiencies
November 2016
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Flexibility for all applications
New 1290 Infinity II Multicolumn Thermostat with space
for up to eight short columns, four 30 cm columns or
any combinations plus column selection valves allows
simplest method-switching.
November 2016
26
Flexibility for all applications
Wide column temperature range of the 1290 Infinity II
Multicolumn Thermostat from 4 to 110 °C to tune
selectivity or speed.
November 2016
27
A new level of usability
1290 Infinity II Multicolumn Thermostat
• Agilent A-Line Quick Connect UHPLC column
fittings for dead-volume-free fluidic connections
• New multicolumn thermostat with simplified access
and space for up to 8 columns
• Easy-to-install Quick Change heat exchangers with
volumes of 1.0, 1.6 or 3.0 μL
• Quick-Change valves for straightforward
reconfiguration of your switching schemes
Graphical sample entry for easy
and fast sequence setup
November 2016
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A new level of usability
New Agilent A-Line Quick Connect UHPLC column fittings
for truly dead-volume-free fluidic connections
• Tool-free connection up to 1300 bar
• Removable and re-usable for all column types
• No overtighting of threads and damaging columns
November 2016
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Designed-In Supplies for 1290 Infinity II
The Agilent A-Line Quick Connect Fitting
November 2016
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• Finger tight up to 1300 Bar
• Spring loaded design
• Design with lever does not damage the capillary
• High efficiency for everybody
• Leak free and reliable connection
• Multiple connections
• Ease of use for non experts
• ZDV connection for all column types
• Removable and reusable
Features
Advantages
Benefits
LE
SS
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RE
SS
MO
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SU
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SA
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-Lin
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Designed-In Supplies for 1290 Infinity II
The Agilent A-Line Quick Turn Fitting
November 2016
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• Finger tight up to 600 Bar, with a wrench up to 1300 Bar
• Spring loaded design
• Design does not damage the capillary
• Leak free and reliable connection
• Multiple connections
• ZDV connection for all column types
• Removable and reusable
Features
Advantages
Benefits
Swagelok
compatible
A new level of usability
New 1290 Infinity II Multicolumn Thermostat with
simplified access and space for up to eight columns
November 2016
32
A new level of usability
New 1290 Infinity II Multicolumn Thermostat with
simplified access, easy and flexible to install Quick-
Change heat-exchangers and Quick-Change Valves. Plus,
unmatched ease of column installation – done in seconds!
November 2016
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A new level of usability
New easy-to-install Quick Change heat exchangers with
volumes of 1.0, 1.6 or 3.0 μL.
November 2016
34
A new level of usability
Increased Quick-Change valve portfolio for straightforward
reconfiguration of your flow path.
(1290 Infinity TCC shown)
November 2016
35
Designed-In Supplies for 1290 Infinity II
The Agilent A-Line Stay Safe Cap
November 2016
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• Tight system
• Free rotating cap
• Ergonomic design
• Time strip on venting valve
• Health lab environment
• Easy solvent refill
• Fast tube connection
• Efficient use of valve
• No solvent vapors in the lab
• No twisted solvent tubes
• Hand tight connection
• Information on valve usage
Features
Advantages
Benefits
A-Line Stay Safe Cap Starter Kit 5043-1222
Includes
• 3 caps with 1port
• 1 cap with 2 ports
• 4 venting valves
• 4 fittings
Available as an
instrument option
November 2016
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• Robust design
• Adjustable shelves
• Handle grip
• Power strip
• Container for waste bottle
• Stable system
• Easy LC stack customization
• Customer self-assembling
• No risk when moving
• High flexibility
• Easy handling
Features
Advantages
Benefits
Designed-In Supplies for 1290 Infinity IIThe Agilent A-Line Mobile Flex Bench
POIS
Opportunity
5043-1252 A-Line Mobile Flex Bench
79cm
165cm
79cm
CPL: November 01
More automation capabilities
1290 Infinity II Multisampler
• Injector programming for simple sample preparation
steps such as dilution, mixing or derivatization
1290 Infinity II Multicolumn Thermostat
• Quick-Change valves for column and solvent
selection, column regeneration, or sample clean-up
and enrichment
Method Scouting Wizard for easy
method & sequence generation for
column/solvent screening
BlendAssist software for
convenient, accurate and precise
additive or buffer blending
November 2016
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Detection capabilities
1290 Infinity II Diode Array Detector
• 60 mm Max-Light Flow Cell with Optofluidic
Waveguides
• 30 x wider linear range for samples with
widely different concentration levels with
HDR-DAD
1290 Infinity II ELSD
• 9 x wider linear range for samples with widely
different concentration levels
• Detection of compounds without chromophor
1290 Infinity II VWD
• 240 Hz data rate
• Dual wavelength capability
Unique detection capabilities reveal more
November 2016
39
Leading 2D-LC Solutions
Most versatile ISET technology and methoddevelopment solutions
Most robust Long Life pumphead technologyand Mixing
Maximum Sampling Capacity with Minimum Benchspace Requirements
High Dynamic Range DAD with High Sensitivity Maxlight Flow Cell Technology
The Agilent 1290 Infinity II LC - Leading in U(H)PLC
Technology
1290 Infinity II LC
November 2016
40
Innovation: 1290 Infinity II 2D-LC SolutionSquare your Separation
1290 Infinity II 2D-LC Solution
• Analytical Efficiency
Unmatched levels of separation
power to solve your most
complex separation problems
• Instrument Efficiency
Most easy-to-use software and
easiest start into 2D-LC with
Easy Starter Kit
• Laboratory Efficiency
Fully scalable system with
automatic switch between 1D-
LC, (Multiple) Heart-Cutting,
and Comprehensive 2D-LC
WHY 2D-LC?
From 1D-HPLC to 2D-LC
November 17, 2016
Confidentiality Label
42
General thoughts in separation science
43
„Resolving power is what it is all
about in analytical separation
science.“
November 17, 2016
Taxanes from taxus extractWhere 2D-LC can help
• Taxol® (Paclitaxel) excellent antitumor activity against
breast and lung cancer.
• Content in Taxus brevifolia very low, synthesis very difficult due
to complex structure.
• Monitoring of content in crop cultures highly desired, but
difficult due to complex herbal matrix
Application Note 5991-3576EN.pdf1D-Chromatograms of standard mix and plant extracts
44
November 17, 2016
Bringing in a 2nd Dimension - how?
45
Pump Autosampler Column Detector
„Just like 1D-LC with a chemically selective detector“
DAD = separates in the wavelength-domain
MSD = separates in the mass-domain
„CSD“ = separates in the chemical domain (again...)
Detector
November 17, 2016
Bringing in a 2nd Dimension - how?
46
Pump Autosampler Column Detector
Pump
Detector
Co
lum
n
Valve
(„Sampler“)
1D-LC System
2D-LC System
+
1D-LC System
„Just like 1D-LC with a chemically selective detector“
November 17, 2016
1D-Setup
Switchable 1D/2D SetupChange between UHPLC and 2D-LC without manual Interaction
2D-Setup
47
November 17, 2016
Technical Note 5991-4843EN
What is ISET?- Two components
Page 48
A. Best in class performance of the
1290 Infinity LCBroad power range, ultra-low delay volume,
superior sensitivity, unmatched flow and
composition accuracy
B. The revolutionary ISET emulation
algorithmFull characterization of target LC systems
Creation of emulation function
FA
Peak 3
Overlay of 10 runs
RSD = 0.005 %
November 2016
What is ISET?
With ISET – part of the 1290 Infinity II pumps – you can
execute any legacy HPLC or latest UHPLC method while
delivering the same chromatographic results.
ISET eliminates deviations in resolution and retention times
when your laboratory deploys methods developed originally
on other HPLC or UHPLC systems.
November 2016
49
Intelligent System emulation technology- How it works (1290 to 1200)
Page 50
Programed gradient
1290 gradient
1200 gradient
0 0.5 1 1.5 2 2.5 3 3.5
0
50
100
150
200
250
300
350
400
min
mAU
Injection
Same gradient conditions by moving
the gradient and by emulating the
gradient mixing behavior.
November 2016
Select pump
Select sampler
Page 51
Intelligent System Emulation Technology ISETImplementation
November 2016
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Intelligent System Emulation Technology ISET- How to emulate Waters Alliance?
Select vendor
Select system
Select sample
loop
November 2016
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ISET not only takes care for the various delay volumes in
different LC systems but also for different mixing behavior
– this is only available from Agilent and make instrument-
to-instrument method transfer easier than ever before.
Non-disruptive transition by ISET
November 2016
54
Agilent innovative ISET technology allows you to use legacy
methods of virtually any LC and achieve the same results on
latest 1290 Infinity II LCs. You can retire your old systems
without retiring your precious methods.
All 1290 Infinity II pumps have ISET included.
Non-disruptive transition by ISET
November 2016
55
Unique detection capabilities reveal more
The 60 mm path length of the Agilent Max-Light flow cell
enables the 1290 Infinity II DAD to achieve typical noise
levels of less than ± 0.6 μAU/cm for highest detection
sensitivity and lowest detection limits.
1290 Infinity II DAD with
High Sensitivity cell
Conventional detector
November 2016
56
Effects of path length increase
Peak Dispersion
60 mm pathlength
large geom. cell volume (78 µL)
lower light transmission
- Loss of resolution
- Loss of signal height
10 mm pathlength
small geom. cell volume (13 µL)
high light transmission
Conventional flow cells:
60 mm pathlength
4 µL σV dispersion volume
Max-Light High Sensitivity cell:
Optofluidic waveguides
(total internal reflection)
Unique detection capabilities reveal more
For your samples with widely different concentration levels,
the 1290 Infinity II High Dynamic Range DAD solution
offers a 30x wider linear range and up to 30x higher sensitivity,
allowing you to detect and quantify all components within a
single run.
November 2016
58
Unique detection capabilities reveal more
For your samples with widely different concentration levels,
the 1290 Infinity II High Dynamic Range DAD solution
offers a 30x wider linear range and up to 30x higher sensitivity,
allowing you to detect and quantify all components within a
single run.
November 2016
59
Introducing Agilent InfinityLab
Combined with Agilent OpenLAB Software and Agilent CrossLab Services & Support, Agilent
provides you with complete Liquid Chromatography solutions which make a real difference.
OpenLAB
Software solutions to capture,
analyze, and share analytical data.- Chromatographic Data Systems
- Enterprise Content Management
- Electronic Notebook
- MassHunter MS Solutions
InfinityLab
LC components designed
to perfectly fit together.- Instruments
- Columns
- Supplies
CrossLab
Serving more than 260000 labs
around the globe.- Instrument repair and maintenance
- Compliance Solutions
- Training and Consulting
- Laboratory Enterprise Solutions
November 2016
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Society Needs More Results for a Better Quality of Life
November 2016
61
Data Analysis and Interpretation Reporting SamplePreparation
Chromatography
Today’s workflow
Highlights
Agilent InfinityLab
LC Series enables
more with less!
Future workflow
Data Analysis and Interpretation Reporting SamplePreparation
Chromatography
The Story of the
InfinityLab LC Series
November 2016
62
Question?