Laser microdissection case study

MCB 7300

Laser-Capture Microdissection, a Tool for the Global Analysis of Gene

Expression in Specific Plant Cell Types:

Identification of Genes Expressed Differentially in Epidermal Cells or

Vascular Tissues of Maize

Mikio Nakazono, Fang Qiu, Lisa A. Borsuk and Patrick S. SchnableThe Plant Cell, Vol. 15, No. 3 (Mar., 2003), pp. 583-596

Overview

• RNA isolation from different plant cell types for microarray analysis

• Protocol changes: cryoprotectant; coagulating fixative (ethanol:acetic acid)

Yields