Download - ÄKTA avant - ebiotrade.com avant customer...50 and 70 on ÄKTA avant 150 • User guidance in UNICORN
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Version2.0
Evolution of ÄKTA™
1996 -
2010
1996 -
ÄKTAexplorer™
1997 -
ÄKTApurifier™
1998 –
ÄKTAFPLC™
1999 -
ÄKTAprime™
2000 –
ÄKTA OligoPilot
2001 –
ÄKTA add on´s
2002 –
ÄKTA Crystal and ÄKTApilot™
2004 -
ÄKTAxpress™
2005 –
ÄKTAcrossflow™
2006 –
New ÄKTApurifier
2009 -
ÄKTAmicro™
2009/10 ÄKTA avant
1982 –
FPLC ™
and Mono Q/S
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Version 4.0
Introducing the next generation of ÄKTA™Within biopharmaceutical process development there are mounting pressures to constrain costs while overcoming increasingly tough competition, regulations, and guidelines. At the same time, your overriding goal is to achieve swift and secure handover of robust processes.
•
ÄKTA is the Swedish word for “true”
or “real”
•
ÄKTA systems are used by 100,000 researchers globally
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ÄKTA avant is the result of meticulous research and close dialogue with you
•
Setting the new standard for fast and secure process development
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Version 4.0
ÄKTA avant 150ÄKTA™
avant 25
•
Screening in small columns•
Method optimization•
Challenging purifications•
Small amounts of sample•
High res. prep. purification•
Optimized for column size i.d. 5-16 mm
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Scale-up process•
Robustness testing•
Trouble shooting (scale-down)
•
Fine-tuning of process•
Optimized for column size i.d. 10-50 mm
Two versions for maximized performance
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Version 4.0
A complete workflow for Process development
HTPD-
Media screening-
Condition screening
ÄKTA™
avant 25 ÄKTA avant 150 ÄKTApilot™
ÄKTA ready
ScreeningMethod optimizationColumn size i.d. 5-16 mm
Scale-up process Fine-tuningRobustness testingColumn size i.d. 10-50 mm
Scale-up optimized processSanitary designColumn sizes from i.d. 50 mm
PreDictor™
Predict Optimize Scale-up Produce
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Version 4.0
Inspired to be the icon of process development
Speed:
Achieving robust, well characterized processes consumes time. ÄKTA™
avant overcomes this, enabling quicker insight and allowing you to get more done in the same time frame.
Scalability:
ÄKTA avant enables you to achieve direct scalability while
systematically anticipating errors to prevent any negative impact on safety, consistency, or economy of the full-scale separation.
Security:
ÄKTA avant allows you to obtain reliable results while working in
a
compliant, error-free manner with complete sample security.
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Version 4.0
ÄKTA™
avantSetting the future industrial standard
Inspired
speed
Inspired
security
Inspired
scalability
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Version 4.0
SpeedÄKTA™
avant has the capacity to significantly shorten the process development time, allowing you to get your products to the market faster
Maximum information, minimum experiments
with DoE
Automation with BufferPro
Ease-of-use with Method editor
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Version 4.0
Design of ExperimentsDoE
is an organized, statistical approach that varies multiple factors simultaneously significantly reducing the number of experiments required. The model is validated, used
to predict the response to untested factor settings, and produces tools to facilitate decision making
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Version 4.0
DoE
reduces your costDesign of Experiments allows the maximum amount of information to be obtained from a minimum number of experiments. By improving efficiency, both time and money are saved
•
Design of Experiments (DoE) is integrated in UNICORN™
6
•
Reach the same result in 16-32 experiments as you would with 128 experiments using the traditional approach!
DoE
Structured approach
Traditional approach
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Version 4.0
BufferPro
–
Automatic buffer preparationÄKTA™
avant includes BufferPro, truly automated on-line buffer preparation -
saves 30 min per buffer and pH-value!
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Completely automated, advanced on-
line buffer preparation
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Buffers are made in analogy with manual preparation: corresponding acids or bases used for pH adjustment
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Actual mixing ratios and buffer recipes are provided
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Allows for easy and quick pH-scouting
pH 5.5pH 5.4pH 5.3
16 pH steps 0.1 units apart,
with no manual buffer titration at all!
HiScreen™
Capto™
SpH from 4,0 to 5,5 using
the CIEX mix buffer
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Version 4.0
Increased ease of use in UNICORN™UNICORN 6 includes a new Method Editor, replacing the method wizard. No new text programming is required, new components immediately ready -
save a day’s work!
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Contains all instructions used for controlling the chromatographic run
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New user interface for easy viewing and editing of run properties
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Use pre-defined methods / phases or program your own
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Easy and flexible method creation -
Drag and drop from the phase library
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Automatic calculation of volume, pressure and flow rate settings for columns
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Version 4.0
System & sample pumps set up to save timeÄKTA™
avant sample and system pumps have the same technical design and run independently, no re-plumbing is necessary, -
save a day’s work per week!
Save time on …
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Column cleaning
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Equilibration
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Regeneration
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Injection valve configuration
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pH measurement in/out of line
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Flow restrictor in/out of line
•
Software reprogramming
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Version 4.0
ÄKTA™
avantSetting the future industrial standard
Inspired
speed
Inspired
security
Inspired
scalability
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Version 4.0
ScalabilityÄKTA™
avant ensures smooth transition from product discovery to production through direct, reliable scalability using the same media
UNICORN™
6 -
A higher standard of intelligence.
Exploit the advantages of modern BioProcess™
media
Scalability from start to finish
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Version 4.0
Increase your productivity with high flow mediaÄKTA™
avant has flow rate and pressure specifications that support modern, high flow BioProcess™
media such as MabSelect™
and Capto™, allowing for smooth scale-up
•
Direct scalability to process scale, using the same media
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Increased productivity with high dynamic binding capacity at high flow
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Increased yield with rapid mass transfer
•
Reduced process time with high volume throughput
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Cost-effective processing
Full support of modern media…Screening, column id 5-16 mm: 20-25 ml/minScale-up, columns id 16-50 mm: 120-130 ml/min
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Version 4.0
0
0,5
1
1,5
2
2,5
3
0 20 40 60 80 100 120 140 160
Flow rate (ml/min)
Back
pre
ssur
e (M
Pa)
•
ÄKTATM
avant
150 offers higher flow rates and lower back pressure than ÄKTAexplorer
100
•
No need for re-plumbing when changing application
•
Perfect fit with modern BioProcessTM
media
Any application without re-plumbingÄKTA avant
allows higher flow rates and lower back pressure, supporting modern BioProcess
media
ÄKTAexplorerTM
100
ÄKTA avant 150
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Version 4.0
Save time with automatic conversion of methods between different
scales
•
Quick and easy rescaling between ÄKTA™
avant 25 and 150
•
Automatic adjustment of system specific settings
Seamless scalability in UNICORN™
6
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Version 4.0
Dedicated column formats for method screeningUse HiScreen™
pre-packed columns -
save a day’s work and make scale up easy!
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Save time on packing , preparation of gel, hardware and solutions, column testing and possible re-packing
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Easily connected in series
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Scalable to BioProcess™ columns packed with the same media using the same process fluid velocities
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19 different BioProcess
media available
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10 cm bed height -
ideal for method optimization and parameter screening
Reliable, reproducible and easy to use!
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Version 4.0
Pressure stable empty columnsPressure stable HiScale™
columns are compatible with modern BioProcess media
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Pressure stable up to 20 bar (2 MPa)
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Complements the XK column series –
equivalent wet parts
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Available with three different inner diameters (16, 26 and 50 mm) and two different lengths (20 and 40 cm)
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Double flow for column packing on ÄKTA™
avant 25 and 150 (50 and 300 ml/min)
Robust, intuitive design and easy to use!
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Version 4.0
Predictable scale up to production scaleReproducible column packing and convenient scale-up with ÄKTA™
avant 150 and AxiChrom™
•
The AxiChrom
column platform simplifies column handling at scales from process development to full-scale production
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Intelligent Packing of AxiChromTM
50 and 70 on ÄKTA avant 150
•
User guidance in UNICORN™
6 ensures optimally packed beds and decreases user dependence
•
Process chromatography is made easier, safer and more efficient!
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Version 4.0
ÄKTA™
avantSetting the future industrial standard
Inspired
speed
Inspired
security
Inspired
scalability
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Version 4.0
SecurityÄKTA™
avant incorporates cutting-edge functionality critical to achieving real security throughout the development process
Cooled fraction collector for true sample integrity
Reliable column protection with
differential pressure measurement
UniTag scanning for complete traceability
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Version 4.0
Fraction collector for optimal securityHassle-free overnight running, outstanding reproducibility and sample security!
•
Temperature control (6
to 20°C) allows samples to be cooled and protected
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Cassettes and rack trays available for micro titer plates up to 250 ml bottles
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Load up to six cassettes in any combination
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Automatic detection of tube rack type
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Spill-free fractionation
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Version 4.0
Precolumn
pressure and delta pressure (ΔP) For increased operational safety, the pressure difference over the packed media bed (ΔP) is continuously measured
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Pressure sensors integrated into the column valve
−
Before the column to protect column hardware
−
After the column, the pressure difference is calculated over the packed media bed (ΔP)
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Automatic pressure-flow control to prevent overpressure
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Pressure sensors are also connected to the system and sample pumps
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Version 4.0
Column handling optimized for sample securityRemove the risk of failed runs due to column performance, lifetime expiry &/or misuse, by keeping track of the columns with the Column Logbook and UniTag
labels
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Identification -
Column type and ID identified through matrix code
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Traceability -
View column data and run history
•
Security -
Set limits for different parameters e.g. allowed number of runs between CIP or Column Performance Test
Easy identification, automatic traceability and outstanding
security of operation!
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Version 4.0
ÄKTA™
avantSetting the future industrial standard
Inspired
technology
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Version 4.0
System orientation
Interactive instrumentdisplay delivers realtimeinformation.
Buffer tray for convenient,large capacity storage.
One wet side with modular set-up limits service down-time
Column valve with built-in by-pass and flow direction change.
High precision systempumps with integratedair sensors.
Swivel foot for easy accessto all parts of the instrument.
Cooled,built-in fractioncollector preventslocal heating ofsamples.
Automatic sample applicationwithout re-plumbing.
pH valve with built-in positions for pH calibration and storage.
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Version 4.0
Innovative setup and componentsMinimized down time during service with easily accessible front
mounted units
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One wet working area easily accessed by simply by rotating the unit
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Components have same geometry, the modules can be quickly exchanged
Cabinet with easy access
UV Monitor Mixer
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Mixer sizes 0.6, 1.4 and 5 ml
•
Integrated in-line filter
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No optical fibers accessible eliminating risk of damage -
increased safety
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Easy to change flow cells: 2 and 10 mm
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True optical path length identified automatically
Multi-functional units
Instrument Display
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5,7”
TFT touch screen display
•
Shows selected run data
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Has pause/continue functionality
Optional Components
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Up to 3 extra valves
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Extra air sensor (before sample pump or before column)
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Version 4.0
Sophisticated valve designCompletely redesigned valves -
versatile system setup for easy method development
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Increased productivity and yield is obtained with the column valve: it connects up to 5 different columns and has built-in reversed flow functionality
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Valve w. integrated pH-electrode enables easy calibration and automatic by-pass of electrode
Injection Valve
pH Valve (& Monitor)
Column Valves•
Multiple sample application techniques without re-plumbing
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System pump and Sample pump can be run simultaneously
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Up to five column positions
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Built-in bypass position
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Built-in flow direction change (reversed flow)
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Built-in pressure sensors before and after column
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Built-in bypass of pH flow cell
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Built-in positions for pH calibration and storage
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Built-in bypass of flow restrictor
Multi-functional units
Inlet Valves•
Built-in air sensor useable with all inlets Outlet Valve
•
Waste and Frac
positions
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Up to 10 outlet positions
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Version 4.0
ÄKTA™
avant
•
Shorten process time with modern resins
•
Save 1 day’s work with easy and flexible method creation and reduce learning time to less than half!
•
Apply DoE
to significantly reduce your development costs
•
Save 30 min per buffer and pH value with BufferPro
•
Save 1 day’s work with system & sample pump set up to run independently & with no need for re-plumbing
•
Save 1 day’s work with pre-packed columns
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Limited service down-time with easily accessible wet side & modular set up
•
Save time by running safely over night
•
…
Reducing weeks to days…
…
while giving you outstanding scalability and sample security
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Version 4.0
GE Healthcare Ltd, a General Electric Company. GE, imagination at work and GE Monogram are trademarks of General Electric Company.
ÄKTA, ÄKTAavant, ÄKTAexplorer, ÄKTApilot, BioProcess, MabSelect, MabSelect
SuRe, Capto, HiScreen, HiScale, AxiChromand UNICORN are trademarks of GE Healthcare companies.
“MabSelect
SuRe
Ligand
Restricted License”
and “Cys-rProtein
A Ligand
Restricted License”
are protected by the following patents and equivalent patents and patent applications in other countries: US 5,151,350, US 5,143,844, US 6,399,750, WO 03/00475and EP 1123389. A free, non-transferable limited license to use this product for internal analytical purposes only accompanies the purchase of the product from a GE Healthcare company and its
licensed distributors. Any other use will require a separatelicense from a GE Healthcare company.
All goods and services are sold subject to the terms and conditions of sale of the company within GE Healthcare which supplies them. General Electric Company reserves the right, subject to anyregulatory and contractual approval, if required, to make changes in specifications and features shown herein, or discontinue the product described at any time without notice or obligation.
© 2010 General Electric Company –
All rights reserved.
GE Healthcare Bio-Sciences AB, a General Electric Company.
GE Healthcare Bio-Sciences AB, Björkgatan
30, SE-751 84 Uppsala, Sweden.