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John A.G. Briggs
Retrovirus structure and assembly
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The structural genomics of HIV
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Gag
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Immature virion
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Immature HIV
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Gag cleavage – can be inhibited
p2 p1p17 p24 p7
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maturation
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MMTV
HIV
2 pathways of Retrovirus formation.
Immature virion
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Immature virion
Internal scaffold domainCytoplasmic retention motif
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MMTV
HIV
Envelopment?
Immature virion
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cytosol
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cytosol
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maturation
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Finch/Sundquist lab
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Core formed from ~1200 capsid proteins
Arranged in ~96Å hexagonal lattice
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maturation
What is the structure of the lattice here?
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In-vitro assembledImmature HIV-1
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If it is made from 80A hexagons, with six proteins per hexagon then 3400 ± 200 gag proteins
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Mean mass: 165 ± 29 MDa
3800 ± 700 gag proteins
Scanning Transmission Electron Microscopy
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145nm immature virion formed from ~5000 Gag proteins
Arranged in ~80 Å hexagonal lattice
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Immature virion
Tighter hexagonal lattice with 8 nm unit cell.
~5000 gag proteins form a complete shell for a 145 nm virus
Mature virion
Looser hexagonal lattice with 9.6 nm unit cell.
~1200 CA proteins form the conical core, leaving a large number elsewhere
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VirologyUniversity of HeidelbergUniversity of OxfordCornell University
Hans-Georg KraüsslichBarbara MüllerMarko LampeBärbel GlassMarc JohnsonVolker VogtQuentin Sattentau
Electron MicroscopyUniversity of OxfordMax-Planck Institute for Biochemistry
Stephen FullerKay GrünewaldFriedrich FörsterThomas WilkGiulia Zanetti