John A.G. Briggs
Retrovirus structure and assembly
The structural genomics of HIV
Gag
Immature virion
Immature HIV
Gag cleavage – can be inhibited
p2 p1p17 p24 p7
maturation
MMTV
HIV
2 pathways of Retrovirus formation.
Immature virion
Immature virion
Internal scaffold domainCytoplasmic retention motif
MMTV
HIV
Envelopment?
Immature virion
cytosol
cytosol
maturation
Finch/Sundquist lab
Core formed from ~1200 capsid proteins
Arranged in ~96Å hexagonal lattice
maturation
What is the structure of the lattice here?
In-vitro assembledImmature HIV-1
If it is made from 80A hexagons, with six proteins per hexagon then 3400 ± 200 gag proteins
Mean mass: 165 ± 29 MDa
3800 ± 700 gag proteins
Scanning Transmission Electron Microscopy
145nm immature virion formed from ~5000 Gag proteins
Arranged in ~80 Å hexagonal lattice
Immature virion
Tighter hexagonal lattice with 8 nm unit cell.
~5000 gag proteins form a complete shell for a 145 nm virus
Mature virion
Looser hexagonal lattice with 9.6 nm unit cell.
~1200 CA proteins form the conical core, leaving a large number elsewhere
VirologyUniversity of HeidelbergUniversity of OxfordCornell University
Hans-Georg KraüsslichBarbara MüllerMarko LampeBärbel GlassMarc JohnsonVolker VogtQuentin Sattentau
Electron MicroscopyUniversity of OxfordMax-Planck Institute for Biochemistry
Stephen FullerKay GrünewaldFriedrich FörsterThomas WilkGiulia Zanetti