Download - HPLC
![Page 1: HPLC](https://reader031.vdocuments.mx/reader031/viewer/2022020520/577cc7871a28aba711a13ae1/html5/thumbnails/1.jpg)
In this course you will introduced to ….
![Page 2: HPLC](https://reader031.vdocuments.mx/reader031/viewer/2022020520/577cc7871a28aba711a13ae1/html5/thumbnails/2.jpg)
![Page 3: HPLC](https://reader031.vdocuments.mx/reader031/viewer/2022020520/577cc7871a28aba711a13ae1/html5/thumbnails/3.jpg)
![Page 4: HPLC](https://reader031.vdocuments.mx/reader031/viewer/2022020520/577cc7871a28aba711a13ae1/html5/thumbnails/4.jpg)
![Page 5: HPLC](https://reader031.vdocuments.mx/reader031/viewer/2022020520/577cc7871a28aba711a13ae1/html5/thumbnails/5.jpg)
![Page 6: HPLC](https://reader031.vdocuments.mx/reader031/viewer/2022020520/577cc7871a28aba711a13ae1/html5/thumbnails/6.jpg)
Photodiode arrays (semiconductor devices) are used in the detection unit. A DAD detects the absorption in UV to VIS region. While a UV-VIS detector has only one sample-side light-receiving section, a DAD has multiple (1024 for L-2455/2455U) photodiode arrays to obtain information over a wide range of wavelengths at one time, which is a merit of the DAD.The idea is that spectra are measured at intervals of 1 second or less during separation by HPLC with continuous eluate delivery. If the measurement is performed at a fixed wavelength, components are identified from only their retention time; thus, a minor deviation in retention time can make identification of components difficult. In such a case, the DAD can be used to identify components by a comparison of the spectrum.
![Page 7: HPLC](https://reader031.vdocuments.mx/reader031/viewer/2022020520/577cc7871a28aba711a13ae1/html5/thumbnails/7.jpg)
![Page 8: HPLC](https://reader031.vdocuments.mx/reader031/viewer/2022020520/577cc7871a28aba711a13ae1/html5/thumbnails/8.jpg)
![Page 9: HPLC](https://reader031.vdocuments.mx/reader031/viewer/2022020520/577cc7871a28aba711a13ae1/html5/thumbnails/9.jpg)
Pure Compound For desired use
![Page 10: HPLC](https://reader031.vdocuments.mx/reader031/viewer/2022020520/577cc7871a28aba711a13ae1/html5/thumbnails/10.jpg)
![Page 11: HPLC](https://reader031.vdocuments.mx/reader031/viewer/2022020520/577cc7871a28aba711a13ae1/html5/thumbnails/11.jpg)
![Page 12: HPLC](https://reader031.vdocuments.mx/reader031/viewer/2022020520/577cc7871a28aba711a13ae1/html5/thumbnails/12.jpg)
![Page 13: HPLC](https://reader031.vdocuments.mx/reader031/viewer/2022020520/577cc7871a28aba711a13ae1/html5/thumbnails/13.jpg)
![Page 14: HPLC](https://reader031.vdocuments.mx/reader031/viewer/2022020520/577cc7871a28aba711a13ae1/html5/thumbnails/14.jpg)
![Page 15: HPLC](https://reader031.vdocuments.mx/reader031/viewer/2022020520/577cc7871a28aba711a13ae1/html5/thumbnails/15.jpg)
Normal Phase Reversed Phase
Stationary phase Polar (silica gel) Non-polar (C18)
Mobile phase Non-polar (organic solvents)
Polar (aqueous/organic)
Sample movement Non-polar fastest Polar fastest
Separation based on Different polarit ies (functionality)
Different hydrocarbon content