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HMG CoA synthase
Philip A. Miller
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Sequence
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Mevalonate Pathway
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Overall Structural Picture
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Kinetics- Difference in Rates with Mutation of Ala110Gly
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Function
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Electron density of the Catalytic loop
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Important Amino Acids and their roles
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Specific Amino Acid Integrations
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Disease/Infection
• Possibility of inhibitors for Mevalonate pathway would serve great as an antibacterial specific for pathogens
• Such as Entherococcus faecalis, Streptococcus pneumoniae, and Staphylococcus aureus
• Common infections from trauma or surgical procedures• Specifically targets pathogenic bacterial, significantly more
effective than commonly used broad-spectrum antibiotics
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Multiple sequence Alignment
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References:• Steussy, C., Robison, A.,Tetrick, A., Knight, J., Rodwell, V.,
Stauggacher, C., Sutherlin, A., (2006) A Structural Limitation on Enzyme Activity: The Case of HMG-CoA Synthase, Biochemistry
• Stuessy, C., Vartia, A., Burgner, J., Sutherlin, A., Rodwell, V., Stauffacher, C., (2005) Biochemistry
• Sutherlin, A., Hedl, M., Sanchez-Neri, B., Burgner, J., Stauffacher, C., and Rodwell, V. (2002) Journal of Bacteriology
• Branden, Carl. Introduction to Protein Structure Garland Publishing, 1991.
• Alberts, B., Bray, D., Lewis, J., Raff, M., Roberts, K., and watson J. D. (1989) Molecular Biology of the Cell, Garland Publishing, New York