Hadar Less
Frontiers in Metabolomics
Integration ofIntegration of
Metabolomics Metabolomics & &
TranscriptomicsTranscriptomics
Information FlowInformation Flow
DNA mRNA Protein Reaction
Integration:Integration:
The whole is larger then The whole is larger then the sum of its parts.the sum of its parts.
The combining and coordinating of The combining and coordinating of separate parts or elements into a separate parts or elements into a unified whole.unified whole.
The Holy GrailThe Holy Grail
Identify function of unknown Identify function of unknown genegene
Identify unknown metaboliteIdentify unknown metabolite
Cluster genes & metabolites Cluster genes & metabolites (pathways)(pathways)
Biological DrawbacksBiological Drawbacks
Several genes encode the same Several genes encode the same enzymeenzyme
One enzyme work on different One enzyme work on different substratessubstrates
One substrate participate in One substrate participate in different reactionsdifferent reactions
Technical DrawbacksTechnical Drawbacks
Information gap between mRNA to Information gap between mRNA to reactionsreactions
Measuring snapshots instead of Measuring snapshots instead of dynamicsdynamics
Low variable to observation Low variable to observation ratioratio
Current publicationsCurrent publications
VisionVision
InfrastructureInfrastructure
What can we do?What can we do?
genes compressiongenes compression
G1
Gn
E1
What can we do?What can we do?
Branch point analysisBranch point analysis
M1
M2
M5
M3
M4 M6
M8M7
E12
E25
E68E67E45
E34 E36
Branch point Branch point enzymesenzymes
What can we do?What can we do?
Imitate dynamicsImitate dynamics
ratioratio
timetime
++++++++++
++++
++
What can we do?What can we do?
Construct public datasetsConstruct public datasets
Integration of Integration of Metabolomics Metabolomics
& & TranscriptomicsTranscriptomics
Hadar Less
Frontiers in Metabolomics