George TwaddleProgram Chair for BiotechnologyIvy Tech Community College of Indiana – South Bend
The Ultimate Inquiry
Lessons from the
Genetically Engineered Machine
Competition
Synthetic Biology• New area of biological
research
• Combines science and
engineering
• Design and construction of
artificial biological functions and systems
Genetic Code is UNIVERSAL
-Discovery of “restrction enzymes” allows us to cut and build recombinant DNA and to express new genetic combinations..
Began in January of 2003Independent Activities Period (IAP) at MIT (The students designed biological systems to make cells blink).
A BIT O’ iGEM HISTORY
5 teams in 2004 13 teams in 200532 teams in 2006 54 teams in 2007 84 teams in 2008 112 teams in 2009 130 teams in 2010
2011.igem.org
The International Genetically Engineered Machine competition (iGEM) is the premiere undergraduate Synthetic Biology competition. Student teams are given a kit of biological parts at the beginning of the summer from the Registry of Standard Biological Parts. Working at their own schools over the summer, they use these parts and new parts of their own design to build biological systems and operate them in living cells. This project design and competition format is an exceptionally motivating and effective teaching method.
What’s an “iGEM?”
Kit of biological parts
at the beginning of
the summer.
Build biological systems
out of the parts or new
parts that they create and
operate them in living cells
Come to a “Jamboree”
at the end to show-off
and see who is most innovative.
An undergraduate synthetic biology annual competition
Tom Knight“BioBrick” inventorGinko BioWorks
Randy RettbergiGEM Competition“Open WetWare” Drew Endy
BioBricks Foundation
iGEM MIT Paternity
THE “BIOBRICK”A standard for interchangeable parts.
For building complex biological systems in cells
Standards achieved through unique “prefix” and “suffix” restriction sites.
Elements of the Competition
Team wiki and Team Parts Pages
Poster presentation
Platform presentation
•Track Award Winners: Best Food or Energy Project: BCCS-Bristol•Best Environment Project: Peking•Best Health or Medicine Project: Washington & Freiburg Bioware (Tie)•Best Information Processing Project: ETHZ Basel & Tokyo Tech (Tie)•Best Manufacturing Project: MIT•Best New Application Area: Slovenia•Best Foundation Advance: Paris Liliane Bettencourt•Best Software Tool: USTC Software
•Special Prizes Winners: Best BioBrick Part, Natural: Minnesota (BBa_K311004)•Best New BioBrick Part or Device, Engineered: Slovenia (BBa_K323135)•Best Human Practices Advance: Imperial College London•Safety Commendation: SDU Denmark & VT-ENSIMAG Biosecurity (Tie) •Best Experimental Measurement: Duke•Best Model: Edinburgh•Best Wiki: Cambridge & Imperial College London (Tie)•Best Poster: KIT-Kyoto•Best Presentation: TUDelft•iGEMers Prize: Cambridge, Imperial, MIT, EPF Lausanne, & Slovenia
Medals and Awards
GoldSilverBronze
Inquiry-Based Learning“A student-centered / teacher-guided instructional approach that
Engages students in investigating
Real World Questions that they choose within a broad thematic framework.
Students acquire and analyze information, develop and support propositions, provide solutions, and design technology that demonstrate their thinking and make their learning visible.” Northeastern Illinois University Dept. of Eduction
What’s your Problem?Food?Energy?
Environment?Health and Medicine?
Industrial & Biomanufacturing?
Software?
Process Advance?
Team Project Description
Aberdeen AyeSwitch Genetic toggle
BCCS-Bristol agrEcoli Soil Fertility Sensor
Brown Light Pattern Control of Cell Circuits
Caltech Bioplastic printer from soybeans and E. coli
EFP-Lusanne Asaia GM bacteria antimalarial
ETHZ-Basel E. Lemming Remote contolled E. coli
Gronigen Hydrobiophilm Synthetic hydrophobic surface
Harvard iGarden Plant Engineering Toolkit
HokkaidoU_Japan Dr. E.coli Protein microinjector
IvyTech-SouthBend To Swim or Not to Swim
P. Aeruginosa biosensor
Lethbridge Bioremediation of tailing ponds
Mexico Very Cool E. coli
Produce antifreeze ,15C
Missouri_Miners Electric E. coli E.Coli Fuel Cell
Newcastle BacillaFilla Bacteria concrete crack filler
UT-Tokyo E. Coli Solves Sudoku
EducationCommunit
y Industry
Local Gov
State Gov.
Federal Gov.
Foundations
Media
BioFuture: Partnerships in Biotechnology
Partnerships in the Future
SKILLS AND KNOWLEDGE STANDARDS FORASSOCIATE DEGREE IN BIOTECHNOLOGY PROGRAMS
IN INDIANA
Created by:Alisa Wright, Baxter Pharmaceutical SolutionsGary Bormett, Dow AgroSciences LCCJose Hanquier, Robert MacKay, Roberta Talmage, Eli Lilly and CompanyWade Lange, Indiana Health Industry ForumAndy Cothrel, Roche Diagnostics Corp. June 30, 2003
Associate of Science orAssociate of Applied Science
In
BIOTECHNOLOGYBloomington Lafayette Lawrence South Bend Terra Haute
iGEM High School Division 2010
• Greenfield Central High School• Warren Central High School• “The DNA Architects of South Bend – Mishawaka (Marian, Penn and Riley High Schools
Illustration creditsLine of students: www.LowestPricedTrafficSchool.comGibson Assembly Song: www.cambrigeigem.orgSynthetic biology figures: “Biology” Campbell and Reece. Pearson, Benjamin-Cummings Publishers (2010) www.masteringbiology.comBacteria: Ministry of Public Works, Canada www.blog.poolcenter.comiGEM team photos, wiki screen shots, etc. 2011.igem.org