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Sterilization
2015 1
General Microbiology 140 MIC
King Saud University Dept. of Bot. & Microbiology
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It is the process which makes things free of microbes .
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Flasks Beakers Pipettes Petri
dishes
Tubes
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Petri dish
A petri dish is a flat dish made of plastic (disposable) or
glass (autoclavable) with a cover that is used to grow
Microorgansim.
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Apparatus Clothes Media Needles
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1- Heat sterilization
2- Chemical sterilization
3-Radiation sterilization
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There are 3 procedures depending on the
tolerance of the material used:
Advantages: It is the simplest, most effective
and inexpensive method.
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1- Heat sterilization
3- Steam or wet
sterilization (Autoclaving).
2-Dry heat sterilization
(hot air oven).
1- Incineration
(Direct heat).
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Bunsen burner produces gas flame which used for heating, sterilization (inoculating loop and needled) .
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Bunsen burners , flames
1- Incineration
(Direct heat):
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This kind of dry heat sterilization is recommended when it is undesirable that steam make contact with the material to be sterilized. This is true for glassware's – glass petri plates, Pipettes as well as for substances like oil, powder, etc. After sterilization wrap them in aluminum foil to avoid recontamination.
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2-Dry heat sterilization
(hot air oven) :
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It includes autoclaves. Used to sterilize culture media,
glassware etc. Usually it operates at 15 lb./sq. inch
steam pressure at 121.5⁰C for 15 min. The advantage of using an autoclave is
that it can reach temperatures higher than boiling water alone, so it can kill not only bacteria but also bacterial spores, which tend to be resistant.
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3- Steam sterilization
(Autoclaving) or wet :
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1- Heat sterilization
2- Chemical sterilization
3-Radiation sterilization
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2- Chemical sterilization
50% Dettol Ethylene oxide
and formalin
Benches /tables
For sterilizing disposable
materials (that can not tolerate
high temperatures).
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1- Heat sterilization
2- Chemical sterilization
3-Radiation sterilization
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Gamma rays,U.V Rays.
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3-Radiation sterilization
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Filtration
Boiling
Cold
temperature
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