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Foundations in Microbiology
Chapter 4
Fifth Edition
Talaro
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Prokaryotic Profiles: the Bacteria and the Archaea
Chapter 4
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Fig. 4.1
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flagella • 3 parts
– filament – long, thin, helical structure composed of proteins
– hook curved sheath – basal body – stack of rings firmly anchored in cell wall
• rotates 360 o
• 12 or many distributed over entire cell • functions in motility
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Flagellar arrangements
1. monotrichous – single flagellum at one end 2. lophotrichous – small bunches arising from
one end of cell 3. amphitrichous – flagella at both ends of cell 4. peritrichous – flagella dispersed over surface
of cell, slowest
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axial filaments
• periplasmic, internal flagella, enclosed between cell wall and cell membrane of spirochetes
• motility
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8 Fig 4.7a b
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fimbrae
• fine hairlike bristles from the cell surface • function in adhesion to other cells and surfaces
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pili
• rigid tubular structure made of pilin protein • found only in Gram negative cells
• Functions – joins bacterial cells for DNA transfer (conjugation) – adhesion
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Conjugation
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glycocalyx
• Coating of molecules external to the cell wall, made of sugars and/or proteins
• 2 types 1. capsule highly organized, tightly attached 2. slime layer loosely organized and attached
• functions – attachment – inhibits killing by white blood cells – receptor
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13 Fig 4.10
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2 Types of Glycocalyx
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Biofilms
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Peptidoglycan
• unique macromolecule composed of a repeating framework of long glycan chains crosslinked by short peptide fragments
• provides strong, flexible support to keep bacteria from bursting or collapsing because of changes in osmotic pressure
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4 groups based on cell wall composition
1. Gram positive cells 2. Gram negative cells 3. Bacteria without cell walls 4. Bacteria with chemically unique cell walls
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Gram positive Gram negative
Fig 4.16
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Gram positive cell wall
• Consists of – a thick, homogenous sheath of peptidoglycan 2080 nm thick
– tightly bound acidic polysaccharides, including teichoic acid and lipoteichoic acid
– cell membrane • Retain crystal violet and stain purple
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Gram positive wall
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Gram negative cell wall
• Consists of – an outer membrane containing lipopolysaccharide (LPS)
– thin shell of peptidoglycan – periplasmic space – inner membrane
• Lose crystal violet and stain red from safranin counterstain
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Gram negative cell wall
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Cytoplasm
• dense gelatinous solution of sugars, amino acids, & salts
• 7080% water • serves as solvent for materials used in all cell functions
Cytoplasmic Membrane Video
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27 Figure 3.10
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Chromosome
• single, circular, doublestranded DNA molecule that contains all the genetic information required by a cell
• DNA is tightly coiled around a protein, aggregated in a dense area called the nucleoid
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plasmids
• small circular, doublestranded DNA • free or integrated into the chromosome • duplicated and passed on to offspring • not essential to bacterial growth & metabolism • may encode antibiotic resistance, tolerance to toxic metals, enzymes & toxins
• used in genetic engineering readily manipulated & transferred from cell to cell
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ribosomes • made of 60% ribosomal RNA & 40% protein
• consist of 2 subunits: large & small • procaryotic differ from eucaryotic ribosomes in size & number of proteins
• site of protein synthesis • All cells have ribosomes.
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ribosomes
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Inclusions, granules
• intracellular storage bodies • vary in size, number & content • bacterial cell can use them when environmental sources are depleted
• Examples: glycogen, polyβhydroxybutyrate, gas vesicles for floating, sulfur and polyphosphate granules
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Inclusions
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34 Figure 3.10
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endospores • Resting, dormant cells • produced by some G+ genera: Clostridium, Bacillus & Sporosarcina
• Have a 2phase life cycle – vegetative cell & an endospore
• sporulation formation of endospores • germination return to vegetative growth • hardiest of all life forms • withstand extremes in heat, drying, freezing, radiation & chemicals not a means of reproduction
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endospores
• resistance linked to high levels of calcium & dipicolinic acid
• dehydrated, metabolically inactive • thick coat • longevity verges on immortality 25, 250 million years.
• pressurized steam at 120 o C for 2030 minutes will destroy.
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endospores
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3 shapes of bacteria
• cocci spherical • bacilli rod • spiral helical, comma, twisted rod, spirochete
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Archaea: the other procaryotes • constitute third Domain Archaea • seem more closely related to Domain Eukarya than to bacteria
• contain unique genetic sequences in their rRNA • have unique membrane lipids & cell wall construction • live in the most extreme habitats in nature, extremophiles
• adapted to heat salt acid pH, pressure & atmosphere • includes: methane producers, hyperthermophiles, extreme halophiles, and sulfur reducers
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