Download - Events at initiation of transcription
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Events at initiation of
transcription
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Abortive initiation assayLet R = RNA polymerase, P = promoter (closed), and Po= promoter (open)
R + P RP RPoATP + UTP*
ApUp*U
kf
kr
KB
Abortive transcripts
[ApUp*U]
time
lag
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Measure kf and KB from lag time vs. 1/[R]
Lag time
Lag time in abortive initiation assay is inversely proportional to [R].
Lag time = KB kf
1kf
1x +1[R]
1[R]Y-intercept =
kf
1
Slope = KB kf
1
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Effect of wild-type and pc mutant λ repressors on activity of PR & PRM
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Effect of Operator Mutations on Transcriptional Control of PR&PRM
OR1-OR2+OR3- OR1+OR2-OR3+
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Effect of λ-pc mutations on KB and k2
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Architecture of λOR
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Mutations in the Activating Region ofλ Repressor
Glu
Glu
Glu
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Mutations in the δ subunit of RNA polymerase that interfere w/λ repressor-mediated activation of PRM transcription
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Effect of mutations in the δ subunit of RNA polymerase on activator-dependent and independent transcription of the lac
promoter
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A model for interaction of the δ subunit of RNA polymerase with λ repressor
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The Awesome Power of Genetics