ERNDIM ADVISORY DOCUMENT OF THE QUANTITATIVE ANALYSIS
OF PURINES AND PYRIMIDINES.
Jörgen Bierau, Ph.D., clinical biochemical geneticist
Laboratory of Biochemical Genetics
Department of Clinical Genetics
Maastricht University Medical Centre
Thanks to Brian Fowler and Jaap Bakker for their advice and help.
This document provides a guideline for the analysis of purines and pyrimidines in
body fluids for diagnostic purposes. First, defects in purine and pyrimidine
metabolism are reviewed along with critical metabolites, second a general method for
the quantitative analysis of purines and pyrimidines using reversed-phase HPLC with
UV-detection is described and third the ERNDIM quantitative purine and pyrimidine
scheme is presented.
1. Introduction to purines and pyrimidines.
Purines and pyrimidines are essential compounds that are encountered in several
forms. One can distinguish bases, nucleosides and nucleotides (figure 1). The former
two are present in body fluids, while the latter group of compounds is only present
intracellularly.
Purines and pyrimidines are the precursors of DNA and RNA, are pivotal for the
regulation of the cell cycle, store and transport energy, are precursors to numerous
cofactors (coenzymes) and are carriers of components of cell membranes and
carbohydrates.
nucleotidenucleoside
base
2’3’
1’4’
αßγ
9
8
7 5
6 1
2
34
Figure 1 Structure of a ribonucleotide
A ribonucleotide is made up of a ribose ring in which the carbon atoms are numbered
1' to 5' (figure 1). In case of a deoxyribonucleotide, the 2' hydroxyl group is reduced
to a hydrogen atom (figure 2). At the 1' position a nitrogenous cyclic base is
covalently bound. At the 5' position of the ribose, an inorganic mono-, di, or
triphosphate ester is bound. The phosphate ions are designated α, β and γ, the α
phosphate group being the one adjacent to the ribose. A nucleotide without the 5'
phosphate ester is called a nucleoside. There are two families of nitrogenous bases,
the purines and the pyrimidines. The most abundant purine bases are adenine and
guanine, their respective (deoxy)ribonucleosides being (deoxy)adenosine and
(deoxy)guanosine. Both DNA and RNA contain adenine and guanine. The pyrimidine
bases are cytosine, uracil and thymine. The corresponding ribonucleosides of cytosine
and uracil are cytidine and uridine, respectively. Both cytidine and uridine are
incorporated into RNA. The pyrimidine ribonucleotides incorporated into DNA are
deoxycytidine and thymidine, the corresponding deoxyribonucleoside of thymine.
N
N
NH
NN
N
OHO
OH
OHOH
OHO
OH
OH
N
N
NH
N
NH2
N
NH
NH
N
NH2
O
N
NH
NH2
O NH
NH
O
O
CH3
NH
NH
O
O
NOOH
OH
N
NN
NH2
NOOH
OH
N
NHN
NH2
O
NOOH
OH
N
NH2
O NOOH
OH
NH
O
O
CH3
purine pyrimidine ß-D-ribose ß-D-deoxyribose
1
2
34
56 7
8
9 1
3
2
4
5
6
adenine guanine
cytosine thymine uracil
deoxyadenosine deoxyguanosine deoxycytidine thymidine
components of natural occuring nucleosides
natural (canonical) occuring bases
natural (canonical) deoxynucleosides
1’ 1’2’ 2’3’ 3’
4’ 4’
5’ 5’
Figure 2 Chemical structures of purines, pyrimidines and their structural parts
Purine metabolism
The precursor of all nucleotides is phosphoribosylpyrophosphate (PRPP). PRPP is
synthesised from ribose-5-phosphate and ATP by PRPP synthetase. The central
purine nucleotide IMP is synthesised via a series of ten reactions (figure 3). IMP can
be phosphorylated further to ITP by nucleoside monophosphate kinase (NMPK) and
nucleoside diphosphate kinase (NDPK), respectively. ITP is, however, actively
hydrolysed back to IMP by ITPase. From the branch-point nucleotide IMP, the
adenine nucleotides are synthesised from the intermediate succinyl-AMP, resulting in
AMP. AMP is subsequently phosphorylated to ADP and ATP by AMP kinase and
NDPK, respectively. The other purine nucleotides, the guanine nucleotides, are
synthesised via xanthine monophosphate (XMP), formed from IMP by IMP
dehydrogenase (IMPDH). GMP is subsequently synthesised from XMP by GMP
synthetase. GDP and GTP are synthesised from GMP by subsequent phosphorylation
by GMP kinase and NDPK. IMPDH is the rate-limiting enzyme in the synthesis of the
guanosine nucleotides. The purine deoxyribonucleotides are synthesised by reduction
of ADP and GDP by ribonucleotide reductase.
The purine salvage pathway of the (deoxy)nucleosides is analogous to the pyrimidine
salvage pathway and both share nucleoside monophosphate kinase (NMPK) and
nucleoside diphosphate kinase NDPK. The nucleoside kinases, however, are different.
Adenosine is phophorylated by adenosine kinase (ADK) and dAdo is phophorylated
by ADK, dCK and deoxyguanosine kinase (dGK), which also catalyses the
phophorylation of dGuo. To date, no existence of a human guanosine, inosine or
xanthosine kinase has been reported. In addition to salvage of the (deoxy)nucleosides,
the purine bases adenine, hypoxanthine and guanosine are salvaged to their respective
nucleotides AMP, IMP and GMP by the phosphorybosyltransferases APRT and
HGPRT, respectively. This is in contrast to the pyrimidine bases, which can only be
converted to their corresponding nucleosides and, subsequently, to their nucleotides.
Purines are degraded by the deaminases adenosine deaminase (ADA) and guanosine
deaminase and purine nucleoside phosphorylase to hypoxanthine and xanthine. The
last two compounds are oxidised and yield the endpoint of purine metabolism in man,
uric acid. In many other species, the poorly soluble uric acid is further oxidised by
uricase to form allantoin, which has a much greater solubility.
PRPP
2 ×
Glu
tam
ine
Gly
cine
Aspa
rtate
N5 ,N
10-m
ethe
nyl-T
HF
N10
-form
yl-T
HF
4 ×
ATP
HC
O3-
IMP
Ino
Hyp
oxan
thin
e
Succ
inyl
-AM
P
AMP
Ado
ADP
ATP
dAD
P
dATP
dAM
P
dAdo
RR
XMP
GM
P
Guo
Gua
nine
Xant
hosi
ne
Xant
hine
Uric
aci
d
GD
P
GTP
dGTP
dGD
P
dGM
P
dGuo
RR
Aden
ine
IMPD
H
DN
AD
NA
RN
AR
NA
12
233
33
4
56
77
89
99
99
10 1810 15 18
1111
1111
12
13
14
14
15
16
17
Figu
re 3
: Pur
ine
met
abol
ism
Enzy
mes
IMP
DH
: IM
P d
ehyd
roge
nase
RR
: Rib
onuc
leot
ide
redu
ctas
e
1: G
MP
syn
thet
ase
2: (d
)GM
P k
inas
e3:
Nuc
leos
ide
diph
osph
ate
kina
se(N
DP
K)
4: G
MP
redu
ctas
e5:
Ade
nylo
succ
inat
esy
nthe
tase
6: A
deny
losu
ccin
ate
lyas
e7:
AM
P k
inas
e(m
yoki
nase
, NM
PK
ac
tivity
)8:
AM
P d
eam
inas
e9:
5’-N
ucle
otid
ase
10: d
Guo
kina
se11
: Pur
ine
nucl
eosi
de p
hosp
hory
lase
12: H
ypox
anth
ine-
guan
ine
phos
phor
ybos
yltra
nsfe
rase
(HG
PR
T)13
: Gua
nine
dea
min
ase
14: X
anth
ine
oxid
ase
15: A
do k
inas
e16
: Ado
dea
min
ase
17: A
deni
ne p
hosp
horib
osyl
trans
fera
se(A
PR
T)18
: dC
ydki
nase
12
9
NMPK
NMPK
Figure 3 Purine metabolism
Pyrimidine metabolism
The first three steps in the de novo synthesis of the pyrimidines are catalysed by CAD,
a trifunctional enzyme cluster that contains carbamylphosphate synthetase, aspartate
carbamyltransferase and dihydro-orotase activities (figure 4). Dihydro-orotate is
reduced to yield orotate by dihydro-orotate dehydrogenase. UMP is subsequently
synthesised from orotate by the bifunctional enzyme UMP synthetase, which contains
the orotate phosphoribosyl transferase and orotidine-5'-phosphate decarboxylase
activities. Subsequent phosphorylation of UMP by nucleoside monophosphate kinase
(NMPK) and nucleoside diphosphate kinase (NDPK), respectively, yields UTP. CTP
synthetase (CTPs) catalyses the conversion of UTP into CTP. CDP is a substrate for
ribonucleotide reductase (RR), which catalyses the reduction of all ribonucleoside
diphosphates to the corresponding deoxyribonucleoside diphosphates (dNDP's).
dNDP's are subsequently phosphorylated to dNTP's by NDPK and incorporated into
DNA, with the exception of dUTP. Although UDP is a substrate for RR, dUTP is only
erroneously incorporated into DNA and directly excised by uracil-DNA glycosylase.
Both dUDP and dUTP are a source of dUMP, from which TMP is synthesised by
thymidylate synthase (TS). TMP is the precursor of TTP, which is ultimately
incorporated into the DNA.
Alternatively, pyrimidine nucleotides are synthesised by salvage of uridine and
cytidine. In pyrimidine metabolism, salvage is the phosphorylation of a nucleoside by
a nucleoside kinase. Uridine and cytidine are phosphorylated by uridine/cytidine
kinase (UK), deoxycytidine is phosphorylated by deoxycytidine kinase (dCK) and
thymidine and deoxyuridine are phosphorylated by thymidine kinase (TK). The
reverse reaction is catalysed by enzymes with 5'-nucleotidase activity.
The pyrimidines are degraded via a common pathway. First, cytidine and
deoxycytidine are converted into uridine and deoxyuridine, respectively, by
(deoxy)cytidine deaminase. Thymidine phosphorylase catabolises (deoxy)uridine and
thymidine into uracil and thymine, respectively. Uracil and thymine are degraded in
three steps to ß-alanine and ß-aminoisobutyrate, respectively, by subsequently
dihydropyrimidine dehydrogenase (DPD), dihydropyrimidase (DHP) and ß-
ureidopropionase. The amino groups of the pyrimidine degradation end products are
removed by transamination to yield malonyl semialdehyde and methylmalonyl
semialdehyde, respectively, which are converted into malonyl CoA and
methylmalonyl CoA. Pyrimidine degradation enters the citric acid cycle (TCA cycle)
via conversion of malonyl CoA and methylmalonyl CoA into propionyl CoA and
succinyl CoA respectively.
Inborn errors of metabolism
As is evident from figures 3 and 4, purine and pyrimidine metabolism is an intricate
network of biochemical reactions catalysed by a myriad of enzymes. In theory, any
genetically determined aberration in the activity or function of any of the enzymes in
purine and pyrimidine metabolism can result in an inborn error of metabolism.
However, an inborn error does not necessarily cause overt disease. At present 39
disorders in purine and pyrimidine metabolism have been described. These include
inborn errors of metabolism, pharmacogenetic risk factors and oncological changes.
Inborn errors of purine and pyrimidine metabolism are associated with a great
diversity of clinical symptoms. Therefore, the diagnosis of these defects can be
challenging. As a guideline, the clinical signs and symptoms requiring analysis of
(urinary) purines and pyrimidines are listed in table 1. In general, a symptom from
this list in isolation does not necessarily imply the need for analysis of purines and
pyrimidines. Analysis of purines and pyrimidines is considered based on the whole
clinical presentation and differential diagnosis. The defects that are encountered in
the field of biochemical genetics are listed in tables 2 and 3. For all disorders listed in
tables 1 and 2 confirmatory testing is available by means of enzyme activity
measurements, molecular diagnostics or both.
UM
P
UD
P
UTP
CTP
CD
P
CM
P
dCD
P
dCM
P
dCTP
Urid
ine
Cyt
idin
edC
ytid
ine
dUrid
ine
dTM
P
dTD
PdU
DP
dUM
P
dUTP
dTTP
dUrid
ine
Thym
idin
e
DN
AR
NA
DN
AR
NA
CTP
s
RR
RR
dCK
, 11
Thym
ine
Dih
ydro
thym
ine
N-C
arba
myl
-ß-a
min
oiso
buty
rate
ß-Am
inoi
sobu
tyra
te
TCA-
cycl
e
112
22
22
3dU
MP
34
5
66
66
67
7
8
10, 1
110
, 11
12
1313
14 15
Dih
ydro
urac
il
N-c
arba
myl
-ß-a
lani
ne
ß-Al
anin
e
15
16
16
9
8
19
Ura
cil
OM
P
Oro
tate
Dih
ydro
-oro
tate
Car
bam
yl a
spar
tic a
cid
Car
bam
yl p
hosp
hate
Aspa
rtic
acid
Aspa
ragi
neG
luta
min
e +
HC
O3-
PRPP
17c
17d
17a
181914
11
ENZY
MES
CTP
s: C
TPsy
nthe
tase
RR
:Rib
onuc
leot
ide
redu
ctas
e
dCK
:deo
xycy
tidin
eki
nase
1: U
MP
/CM
P k
inas
e (N
MP
K)
2:N
ucle
osid
e di
phos
phat
eki
nase
(N
DP
K)
3: (d
)CM
Pde
amin
ase
4: T
MP
kin
ase
5:dU
TPas
e
6: 5
’-Nuc
leot
idas
e
7:U
rd/C
ydki
nase
8: (d
)Cyd
dea
min
ase
9:Th
ymid
ylat
e sy
ntha
se
10:d
Thd
kina
se (c
ytos
olic
TK-1
)
11:d
Thd
kina
se (m
itoch
ondr
ialT
K-2
)
12:U
rd p
hosp
hory
lase
13:d
Thd/
dUrd
pho
spho
ryla
se
14:D
ihyd
ropy
rimid
ine
dehy
drog
enas
e
15:D
ihyd
ropy
rimid
ase
16: ß
-Ure
idop
ropi
onas
e
17C
: CA
DC
arba
myl
phos
phat
e sy
nthe
tase
17A
: CA
DA
spar
tate
ca
rbam
yltra
nsfe
rase
17D
CA
DD
ihyd
roor
otas
e
18:D
ihyd
roor
otat
e de
hydr
ogen
ase
19: U
MP
synt
heta
se
Figure 4 Pyrimidine metabolism
Table 1: Symptoms that may point to the need of purine and pyrimidine analysis Anaemia (megaloblastic, haemolytic) Arthritis Autism Automutilation Cachexia, feeding difficulties Cerebral palsy Developmental delay Dysmorphic features Encephalopathy Epilepsy, seizures, fitting Exercise intolerance Gout Haematuria Hepatomegaly Hyperactivity, short attention span Hyperuricaemia Hypo-/hypertonia Immunodeficiency Impaired hearing, deafness Lactic acidosis Lens dislocation Lymphopaenia Microcephaly Mitochondrial DNA-depletion Muscle weakness Psychomotor retardation Nephropathy Nephro/urolithiasis Optic atrophy Renal failure (acute and chronic) Scoliosis Severe combined immunodeficiency Spastic diplegia Splenomegaly T-cell immunodeficiency Tetraparesis
Table 2: Inborn errors of purine and pyrimidine metabolism with urinary biomarkers Purines Index metabolites in urine Disorder OMIM Gene Increased excretion Decreased
excretion Phosphoribosyl pyrophosphate synthetase deficiency
301835311850
PRPS1 orotic acid uric acid
Phosphoribosyl pyrophosphate synthetase superactivity
300661311850
PRPS1 uric acid
Adenylosuccinate lyase deficiency
103050608222
ADSL SAdo, SAICAR
AICAR transformylase/IMP cyclohydrolase deficiency
608688 ATIC AICAR
Hypoxanthine guaninine phosphoribosyl transferase deficiency
30032230032330800
HPRT hypoxanthine, xanthine, uric acid
Adenine phosphoribosyl transferase deficieny
102600 APRT 2,8-dihydroxyadenine
Adenosine deaminase deficiency
102700608958
ADA (deoxy)adenosine
Purine nucleoside phosphorylase def.
613179164050
PNP (deoxy)inosine, (deoxy)guanosine
Xanthine dehydrogenase def. Isolated
278300607633
XDH hypoxanthine, xanthine
Uric acid
Xanthine dehydrogenase def. combined with aldehyde oxydase deficiency
602841 AOX1 hypoxanthine, xanthine
Uric acid
Xanthine dehydrogenase def. due to Molybdenum cofactor deficiency
252150603707603708603930
MOCS1MOCS2GEPH
hypoxanthine, xanthine S-sulfocysteine, sulfite, thiosulfate
Uric acid, cystine
Table 2 continued Pyrimidines Index metabolites in urine Disorder OMIM Gene Increased
excretion Decreased excretion
Orotate phosphoribosyl transferase deficiency (Uridine-5’-monophosphate synthase deficiency )
258900 UMPS orotic acid
Thymidine phosphorylase deficiency
603041131222
TYMP thymidine, deoxyuridine
Dihydropyirimidine dehydrogenase deficiency
274270612779
DPYD uracil, thymine
Dihydropyrimidinase deficieny 222748 DPYS dihydrouracil, dihydrothymime
Beta-ureidopropionase deficiency
613161606673
UPB1 N-carbamoyl-ß-alanine N-carbamoyl-ß-amino-isobutyric acid
ß-alanine-α-ketoglutarate aminotransferase deficiency
237400 ß-alanine
ß-aminoisobutyrate-pyruvate aminotransferase deficiency
210100 ß-aminoisobutyric acid
Uridine-5’-monophosphate hydrolase superactivity (pyrimidine specific 5’-nucleotidase superactivity)
266120606224
UMPH1 uric acid
Table 3: Inborn errors of purine and pyrimidine metabolism without specific urinary biomarkers Disorder OMIM Gene Biomarker Adenosine-5’-monophophate deaminase deficiency (Myoadenylate deaminase def.)
102770 AMPD1 Ischemic forearm test: decreased NH3, increased creatine kinase after exercise
Inosine-5’-triphosphate pyrophosphohydrolase deficiency
147520 ITPA Intracellular accumulation of inosine-5’-triphosphate (ITP)
Deoxyguanosine kinase deficiency 251880601456
DGUOK Hepatocerebral mtDNA depletion, increased plasma lactate
Thymidine kinase 2 deficiency 609560188250
TK2 Muscular mtDNA depletion, increased plasma lactate
Thiopurine S-methyltransferase deficiency
610460187680
TPMT Inability to methylate thiopurines
Uridine-5’-monophosphate hydrolase deficiency (pyrimidine specific 5’-nucleotidase deficiency)
266120606224
UMPH1 Accumulation of pyrimidine nucleotides in erythrocytes
Mitochondrial Ribonucelotide Reductase subunit 2 deficiency
604712 RRM2B Hepatocerebral mtDNA depletion, increased plasma lactate
2. Recommendations for the analysis of purines and pyrimidines in
biological fluids
The most commonly used method to analyse purines and pyrimidines in biological
fluids is reversed phase high performance (pressure) liquid chromatography (HPLC)
with UV-Visible detection. The preparation of the samples varies, some protocols
require only dilution of the sample while other protocols prescribe solid phase
extraction in order to concentrate the compounds of interest prior to analysis.
Alternatively, some compounds are also detected by GC-MS in the analysis of
organic acids.
At present liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS)
is becoming more and more the first-line analytical tool for the analysis of many
compounds and groups of compounds, including purines and pyrimidines. LC-
MS/MS allows automation and thus greater sample throughput and a broader
spectrum of analytes within one run, e.g. HPLC-MS/MS makes it possible to analyse
all purine and pyrimidine metabolites in one run. Contrary to UV-Vis detection, the
lack of UV-absorption of some compounds is irrelevant for MS/MS-detection.
Unfortunately, this technique requires substantial investments and is therefore not yet
available to all laboratories working in the field of biochemical genetics. Therefore, a
standard procedure using HPLC with UV-detection is described in this document. For
a method using LC-MS/MS, please refer to van Kuilenburg et al. in “Laboratory
guide to the methods in biochemical genetics”, Blau et al. eds. pp 725-737.
Analysis of purines and pyrimidines in biological fluids by HPLC with UV-
detection.
General aspects
Laboratory:
Fume hood should be available.
Method/instrumentation:
Automated reversed-phase liquid chromatography with UV-Vis detection.
High standard high performance (pressure) liquid chromatograph equipped with at
least dual wavelength detection (a Diode Array Detector is preferable) and
thermostated column holder and thermostated auto-sampler unit.
Column: Nucleosil C18 column (250 mm × 4.6 mm × 5 µm). Guard-column: standard
reversed-phase guard column. Mobile phase: solution A: 350 ultra-pure water: 335
acetonitrile: 400 methanol (v:v:v), solution B: 0.1 mol/l KH2PO4 pH 5.0.
Linearity Approximately 300 – 6000 µmol at λ = 254 nm, depending on the compound. Sensitivity The lower detection limit is approximately 1 – 10 µmol, depending on the compound. Sample volume (absolute minimum): Urine : 50 µl Plasma : 200 µl CSF : 200 µl Additional apparatus and disposable objects required: Plastic safe Lock vials Microcentrifuge Safe Lock Bio vials 1.5 ml Waterbath Autosampler vials Microcentrifuge tube filters Vortex-mixer Micro pipettes Disposable pipette tips
Sample collection and storage
Urine:
Traditionally 24-hour urine collection or overnight collection is preferred for
diagnostic purposes. In practice, many laboratories use urine portions for diagnostic
purposes. No preservatives are added to the sample.
The recommended procedure for 24-hour urine collection is a follows: during the
collection period the urine aliquots are kept refrigerated (4°C) and after completion
the urine is sent to the laboratory in a well-isolated package and stored in the
refrigerator for max. one week at 4°C until analysis or stored frozen at -20°C when
analysis is carried out after more than one week but within 2 months. For longer
period store at -80 °C.
Dipstick tests for nitrite and pH should be carried out directly after receipt of the
urine in order to check for bacterial contamination. In addition qualitative tests
for glucose, reducing substances, sulphite and ketone bodies should be performed.
Analysis should not be performed in severely bacterially contaminated samples (pH>7
and/or nitrite is positive).
Plasma
The analysis of purines and pyrimidine can be performed in plasma obtained from
blood heparin coagulated with heparin as well as EDTA. This can be adjusted
according to local protocols. In the case of capillary blood, clean and disinfect skin
thoroughly before taking the blood sample, to avoid contamination from the skin
surface.
Plasma samples should be stored at –20 º C or at –80 ºC, if stored for a prolonged
period. Plasma samples should be deproteinized before analysis (see below).
CSF
Please refer to your own hospital protocol for the lumbar punction procedure.
CSF samples should be deproteinized before analysis (see below). CSF samples
should be stored at –80 ºC
Sample preparation for analysis
Urine:
Thaw frozen urine sample in a water bath at 37 ºC, and leave at 37 ºC for 30 minutes.
Mix thoroughly. Dilute the urine sample five times in ultra pure water (thus 1+4) and
mix using a vortex-mixer. Usually 50 µl of the urine sample is used. Filter the sample
using a 0.2 µm nylon micro centrifuge filter. Transfer the filtrate to a clean injection
vial.
Plasma and CSF:
Quickly thaw the frozen sample in a water bath at 37 ºC. Place the sample vial on ice
immediately after thawing. From this point on the sample is kept on ice. Gently mix
the sample. To 200 µl of sample 200 µl of 2 mol/l perchloric acid (HClO4) in water is
added. Mix using a vortex-mixer. Leave to stand for 10 minutes on ice in order to
deproteinize the sample. Centrifuge the sample at 20.000 × g at 4 ºC for 10 minutes.
Transfer 300 µl of the supernatant to a clean vial. Add 10 µl of 0.5%(w/v) phenol red
in water and 100 µl of 4 mol/l KOH + 1mol/l KH2PO4 prepared in water. After
thorough mixing, the colour should be of a salmon pink quality. If necessary add more
of the 4 mol/l KOH + 1 mol/l KH2PO4 solution in 10 µl portions to obtain a solution
having the colour described. Leave to stand on ice for 15 minutes. Centrifuge the
sample at 20.000 × g at 4 ºC for 5 minutes. Filter the supernatant using a 0.2 µm
nylon micro centrifuge filter. Transfer the filtrate to a clean injection vial.
Control samples:
Pooled and for some compounds enriched samples are prepared using the same
procedures as for patient samples and included in each series for diagnostic analysis.
Control samples should only be deproteinized together with patient samples.
Note that some purines and pyrimidines have a very poor solubility, e.g. 2,8-
dihydroyadenine and xanthine, and require sonication and basic conditions to
dissolve. Place urine samples in a ultrasonic water bath for 10 minutes after the
thawing procedure described above to increase solubility.
Reagents and buffers:
General remark:Always visually inspect buffers and solutions. Discard if
contaminated.
2 mol/l Perchloric acid (HClO4). Store at 4 ºC for max. 1 year
0.5 % Phenol-red (w/v). Store at 4 ºC for max. 1 year
2.5 mol/l KOH in ultra pure water. Store at 4 ºC for max. 1 year
4 mol/l KOH, 1 mol/l KH2PO4 in ultra pure water. Store at 4 ºC for max. 1 year
Mobile phase solution A: 350 ultra-pure water: 335 acetonitrile: 400 methanol
(v:v:v). Store at ambient temperature for max. 1 year.
Mobile phase solution B: 0.1 mol/l KH2PO4 pH 5.0. Store at ambient temperature for
max. 1 month.
Standards
Internal standards: no internal standards are used in this method.
Calibrators: Aqueous solutions of purines and pyrimidines are used to calibrated
each series of analyses. Typical concentrations of the components in the calibrator
solution are 150-200 µmol/l.
Analysis
Preparation
Mobile phase solutions are used at ambient temperature. The column is thermostated
at 20 ºC. The sample tray of autosampler is kept at 4 ºC. Samples, control samples and
calibrators should be ready for analysis.
Analysis
The elution gradient for diagnostics analysis of purines and pyrimidines should
achieve separation of the compounds to such a degree that they can be distinguished
qualitatively and quantitatively. Since numerous configurations of the equipment
mentioned above exist only an example can be given. This example can serve as
starting point for optimisation of the method using your own equipment.
In the analytical validation the following is essential:
• Visual inspection of the chromatogram (base line, peak areas, elution pattern)
• Verify calibrators with respect to retention times and peak areas.
• Identification of every single analyte should be made with great care
Interpretation and quantification:
Each purine and pyrimidine base has its own characteristic UV absorbance spectrum
which can be used for identification. A library of spectra obtained from pure standards
should be obtained. Peaks are identified based on their retention time and by
comparison of the recorded absorption spectrum to library of spectra. Single-
wavelength UV detection and quantification is usually performed at λ = 254nm. At
this wavelength many substances have an absorbance maximum. Purines also have
an absorption maximum at λ = 280 nm. For practical purposes quantification is often
done at one wavelength (λ = 254nm). 2,8-Dihydroxyadenine is best quantified at λ =
350nm
Concentrations are calculated by an external-standard method. First, a response factor
(Rf, nmol-1) is calculated by dividing the peak area of the standard (As) by the
absolute amount in nmol of the analyte injected on the column (ns).
Rf = As
ns
The concentration of the component in the analytical sample (Ci) is calculated by
dividing the area of the component of interest in the sample by the response factor,
corrected for the injected sample volume.
Ci = f * Ai
Rf
Representitive system configuration and settings
Shimadzu® High Performance Liquid Chromtograph configuration: LC-10 Adv
pump, SIL-10 Adv autosampler, SPD-M10 Avp Diode Array Detector, SL-10Avp
System controller. Additional components: Spark Mistral column oven, Alltech
(mobile phase ) degasser.
Integration software package: Shimadzu Class-VP
Settings chromatograph and gradient: Flow rate: 1 ml/min Gradient mode: linear
Time (min) % Mobile phase solution A % Mobile phase solution B 0 0 100 8 1 99 25 5 95 30 12 88 35 20 80 40 20 80 50 37 63 60 50 50 65 0 100 90 0 100
Diode Array Detector Settings Start wavelength: 190 nm End wavelength: 370 nm Absorbance: 0-60 mAU Wave step: 1 nm Lamp type: deuterium Sampling frequency: 1.5625 s-1 Run time: 60 min Acquisition delay: 0 min Time constant: 0.64 s
Auto sampler Settings: Injection volume: urine: 25 µl plasma: 50 µl CSF: 100 µl Rinsing volume: 200 µl Needle stroke: 35 mm Rinsing speed 35 µl/s Sampling speed: 15 µl/s Purge time: 5 min Rinse mode: After aspiration Rinse dip time : 2 s
Quality control
Recoveries, detection limits and linearity of all purines and pyrimidines have to be
established by analysing urine, plasma and CSF samples before and after enrichment
(standard addition method) with reference compounds to define these parameters in
the relevant biological matrix. Exact retention times and response factors for all
components have to be determined at the wavelength used for quantification. Control
urine or plasma samples are analysed in each series of urine, plasma or CSF analyses
to control the inter-assay reproducibility
All values obtained for samples are registered and compared with the calculated
respectively expected values and in case of deviations of > 2 s.d. the series is
repeated.
Instrument performance is checked by observing the baseline and noise level,
and the signal to noise ratio.
In general:
Analytical detection depends on:
1. Quality and condition of the analytical column.
2. Response factor in the linear area (is different for each component);
3. Performance of the detector.
Any change or adjustment of the system may cause changes to occur in the linear part
of the calibration curve, e.g. new column, new detector lamp.
With every change in the procedure mentioned it is required to check if this influences
the analytical performance. Analysis of the pooled control samples and the calibrators
will make clear if any adjustments need to be made.
External Quality control
Participation in an external QA scheme like the ERNDIM scheme "Purines and
Pyrimidines in Urine" is strongly recommended.
The ERNDIM External Quality control scheme for Purines and Pyrimidines
The ERNDIM (European Research Network for evaluation and improvement of
screening, diagnosis and treatment of inherited Disorders of Metabolism) scheme
"Purines and Pyrimidines in Urine" is organised by SKZL (Stichting
Kwaliteitsbewaking Ziekenhuis Laboratoria) at Nijmegen (NL) on behalf of
ERNDIM. This is a purely quantitative scheme dealing with the technical analysis of
purines and pyrimidines only. The idea is that good technical performance is essential
for diagnostic proficiency. Diagostic proficiency is addressed by ERNDIM in de
DPT-schemes.
Aim and scope of the scheme
The aim of the ERNDIM qualitative Purine and Pyrimidine scheme is to monitor the
analytical quality of the quantitative assay of purines and pyrimidines in urine in
laboratories involved in the screening and diagnosis of patients with inherited
metabolic disorders. This allows the participants to test their analytical methods and
compare their results with their peers. The ultimate objective of an external quality
control scheme is harmonization of the analytical skills of the participants, so that the
same results are obtained in one and the same sample, regardless of the laboratory
performing the analysis or the method used.
Every year, the scheme organizer and the scientific-advisor provide the participants
with information concerning the performance of the participants as a group and each
participant individually. The information on the performance of the group as a whole
is provided by ERNDIM. In each annual report accuracy, recovery, precision,
linearity, interlab CV are discussed.
For each annual series, 8 spiked lyophilised human urine samples are distributed.
Each series consists of 4 randomly distributed pairs, allowing determination of the
precision of a lab. Linearity over the whole relevant analytical range is another
important parameter for analytical quality. This is also addressed in the schemes’
design. A second approach to describe accuracy is the percentage recovery of added
analyte. In this approach it is assumed that the amount of the weighed quantity is the
target value.
The purine and pyrimidine scheme is a purely quantitative scheme dealing with the
performance of the technical analysis of purines and pyrimidines only. Diagnostic
proficiency is addressed by ERNDIM in the diagnostic proficiency schemes.
However, diagnostic proficiency strongly benefits from good technical performance.
ERNDIM achieves to create a comprehensive scheme, bearing in mind that it is not
possible to cover all disorders of purine and pyrimidine metabolism. Some disorders
lead to aberrant intracellular nucleotide levels only and cannot be detected in body
fluids, while other disorders do not have a primary index metabolite. Moreover, not
all inborn errors of purine and pyrimidine metabolism that have biomarkers are
represented in the scheme. It is, of course, the ultimate goal to include all known
measurable relevant compounds in the scheme at a reasonable price. The compounds
included in the 2010 series are: Orotic acid, Orotidine, Uracil, Adenine, Thymine,
Uridine, Dihydrothymine, Dihydrouracil), Hypoxanthine, Xanthine, Adenosine,
Succinyladenosine, 5-amino-4-imidazolecarboxamide riboside (AICAR), 2,8-
dihydroxyadenine, Guanosine, Inosine, Deoxy-adenosine, Deoxy-guanosine, Deoxy-
inosine, Deoxy-uridine, 5-OH methyluracil, Creatinine, Uric acid and Thymidine. At
present succinylamino-imidazole carboxamine riboside (SAICAR) is lacking in the
scheme. This is something ERNDIM is working on, but this takes time and may prove
not be feasible on short term.
A word on dihydrouracil and dihydrothymine
Dihydrouracil (DHU) is formed from uracil by dihydropyimidine dehydrogenase
(DPYD), and is subsequently, degraded to N-carbamoyl-ß-alanine in a reaction
catalyzed by dihydropyrimidinase. Thymine is degraded to, subsequently,
dihydrothymine and N-carbamoyl-ß-amino-isobutyric acid by the same enzymes.
DHU was added to the purine and pyrimidine scheme in 2005 in order to improve the
diagnosis of dihydropyrimidinase deficiency, in which there is an accumulation of
dihydro-uracil and dihydro-thymine in the body fluids. In the reaction catalyzed by
DPYD uracil and thymine lose their conjugated electron configuration and thus their
UV-activity. Therefore, the quantification of the dihydropyrimidines is more
complicated than the compounds that have UV-absorption and requires different
analytical techniques. The dispersion in the values measured by the participants is
extremely large, especially considering the 2005 and 2006 series. Initially, virtually
all participants used GC-MS for the quantification of DHU. In the 2007 series, results
obtained with LC-MS/MS made their way into the scheme. Still, the overall results
were far from perfect. While GC-MS is often off target, the results obtained by liquid
chromatography coupled with tandem mass spectrometry are close to the target values
and the dispersion is less extreme. It is the scientific advisor’s opinion that LC-
MS/MS is the superior technique for the quantification of DHU.